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C5A255 (TRUB_THEGJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable tRNA pseudouridine synthase B
EC=5.4.99.25
Alternative name(s):
tRNA pseudouridine(55) synthase
Short name=Psi55 synthase
tRNA pseudouridylate synthase
tRNA-uridine isomerase
Gene names
Name:truB
Ordered Locus Names:TGAM_1972
OrganismThermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) [Complete proteome] [HAMAP]
Taxonomic identifier593117 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus ›

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs By similarity. HAMAP-Rule MF_01081

Catalytic activity

tRNA uridine55 = tRNA pseudouridine55. HAMAP-Rule MF_01081

Sequence similarities

Belongs to the pseudouridine synthase TruB family. Type 2 subfamily.

Contains 1 PUA domain.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA pseudouridine synthesis

Inferred from electronic annotation. Source: HAMAP

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

pseudouridine synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334Probable tRNA pseudouridine synthase B HAMAP-Rule MF_01081
PRO_1000213510

Regions

Domain250 – 32576PUA

Sites

Active site821Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
C5A255 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: 9D3DABBBB17B68A6

FASTA33437,873
        10         20         30         40         50         60 
MARDEVRRIL PADIKREVLI KDEKAETNPK WGFPPERRPM EMHMQFGIIN LDKPPGPTSH 

        70         80         90        100        110        120 
EVVAWIKKLF NLSKAGHGGT LDPKVSGVLP VALERATRVV QALLPAGKEY VALMHLHGDI 

       130        140        150        160        170        180 
PEDKILAVMK EFQGEIIQRP PLRSAVKRRL RTRKVYYIEV LEIEGRDVLF RVGVEAGTYI 

       190        200        210        220        230        240 
RSLIHHIGLA LGVGAHMAEL RRTRSGPFKE DETLVTLHDL VDYYHFWKED GIEEYFRKAI 

       250        260        270        280        290        300 
QPMEKAVEHL PKVWIRDSAV AAVTHGADLA VPGIVKLHKG IKKGDLVAIM TLKDELVALG 

       310        320        330 
KAMMTSGEML QRSKGIAVDV DKVFMPRDWY PRMW

« Hide

References

[1]"Genome analysis and genome-wide proteomics of Thermococcus gammatolerans, the most radioresistant organism known amongst the Archaea."
Zivanovic Y., Armengaud J., Lagorce A., Leplat C., Guerin P., Dutertre M., Anthouard V., Forterre P., Wincker P., Confalonieri F.
Genome Biol. 10:R70.1-R70.23(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 15229 / JCM 11827 / EJ3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001398 Genomic DNA. Translation: ACS34474.1.
RefSeqYP_002960338.1. NC_012804.1.

3D structure databases

ProteinModelPortalC5A255.
ModBaseSearch...

Protein-protein interaction databases

STRING593117.TGAM_1972.

Proteomic databases

PaxDbC5A255.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACS34474; ACS34474; TGAM_1972.
GeneID7987029.
KEGGtga:TGAM_1972.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0130.
HOGENOMHOG000231224.
KOK11131.
OMAVDAICHG.
ProtClustDBPRK04270.

Family and domain databases

HAMAPMF_01081. TruB_arch.
InterProIPR012960. Dyskerin-like.
IPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR004802. tRNA_PsdUridine_synth_B_fam.
IPR026326. TruB_arch.
IPR004521. Uncharacterised_CHP00451.
[Graphical view]
PANTHERPTHR23127. PTHR23127. 1 hit.
PfamPF08068. DKCLD. 1 hit.
PF01472. PUA. 1 hit.
PF01509. TruB_N. 2 hits.
[Graphical view]
SMARTSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMSSF55120. PsdUridine_synth_cat_dom. 1 hit.
SSF88697. PUA-like. 1 hit.
TIGRFAMsTIGR00425. CBF5. 1 hit.
TIGR00451. unchar_dom_2. 1 hit.
PROSITEPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRUB_THEGJ
AccessionPrimary (citable) accession number: C5A255
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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