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C5A1I4

- C5A1I4_THEGJ

UniProt

C5A1I4 - C5A1I4_THEGJ

Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

    Catalytic activityi

    2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
    3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei207 – 2071Proton acceptorUniRule annotation
    Binding sitei209 – 2091SubstrateUniRule annotation
    Metal bindingi233 – 2331Magnesium; via carbamate groupUniRule annotation
    Metal bindingi235 – 2351MagnesiumUniRule annotation
    Metal bindingi236 – 2361MagnesiumUniRule annotation
    Active sitei325 – 3251Proton acceptorUniRule annotation
    Binding sitei326 – 3261SubstrateUniRule annotation
    Binding sitei358 – 3581SubstrateUniRule annotation
    Sitei366 – 3661Transition state stabilizerUniRule annotation

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. oxidoreductase activity Source: UniProtKB-KW
    3. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. AMP catabolic process Source: UniProtKB-HAMAP
    2. carbon fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    LyaseUniRule annotationImported, OxidoreductaseUniRule annotation

    Keywords - Biological processi

    Carbon dioxide fixationUniRule annotation

    Keywords - Ligandi

    MagnesiumUniRule annotation, Metal-bindingUniRule annotation

    Enzyme and pathway databases

    BioCyciTGAM593117:GHFT-1782-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
    Short name:
    RuBisCOUniRule annotation
    Gene namesi
    Name:rbcLUniRule annotationImported
    Ordered Locus Names:TGAM_1751Imported
    OrganismiThermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3)Imported
    Taxonomic identifieri593117 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
    ProteomesiUP000001488: Chromosome

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei233 – 2331N6-carboxylysineUniRule annotation

    Proteomic databases

    PaxDbiC5A1I4.

    Interactioni

    Subunit structurei

    Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

    Protein-protein interaction databases

    STRINGi593117.TGAM_1751.

    Structurei

    3D structure databases

    ProteinModelPortaliC5A1I4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni411 – 4133Substrate bindingUniRule annotation
    Regioni433 – 4364Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1850.
    HOGENOMiHOG000230831.
    KOiK01601.
    OMAiVIVTFRV.

    Family and domain databases

    Gene3Di3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    HAMAPiMF_01133. RuBisCO_L_type3.
    InterProiIPR017712. RuBisCO_III.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view]
    PfamiPF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsiTIGR03326. rubisco_III. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    C5A1I4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADPFSFLCP YLGRKFNKPL FPSFLPPARR LNSRRGENPL RWLRMVEKFD    50
    KIYDYYVDKD YEPNKKRDII AVFRVTPAEG YTIEQAAGAV AAESSTGTWT 100
    TLYPWYEQER WADLSAKAYD FIDMGDGSWI VKIAYPFHAF EEWNLPGLLA 150
    SIAGNVFGMK RVKGLRLEDL YFPEIVLRNF SGPAFGIEGV RKMLEIYDRP 200
    LYGVVPKPKV GYSPEEFEKL AYELLSNGAD YIKDDENLTS PWYNRFDERA 250
    EIVTRVIDKV ENETGEKKTW FANITADIRE MERRLEVLAD LGLKHAMVDV 300
    VITGWGALEY IRDLAADYGL AIHGHRAMHA AFTRNKYHGI SMFVLAKLYR 350
    IIGIDQLHVG TAGAGKLEGG KWDVIQNARI LREETYKPDE NDVFHLEQKF 400
    YGMKAAFPTS SGGLHPGNIE PVIEALGKDI VLQLGGGTLG HPDGPGAGAR 450
    AVRQAIDAIM QGIPLDEYAK THKELARALE KWGHVTPV 488
    Length:488
    Mass (Da):55,159
    Last modified:July 28, 2009 - v1
    Checksum:i60A0F453AC3CDFFC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001398 Genomic DNA. Translation: ACS34253.1.
    RefSeqiYP_002960117.1. NC_012804.1.

    Genome annotation databases

    EnsemblBacteriaiACS34253; ACS34253; TGAM_1751.
    GeneIDi7987470.
    KEGGitga:TGAM_1751.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001398 Genomic DNA. Translation: ACS34253.1 .
    RefSeqi YP_002960117.1. NC_012804.1.

    3D structure databases

    ProteinModelPortali C5A1I4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 593117.TGAM_1751.

    Proteomic databases

    PaxDbi C5A1I4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACS34253 ; ACS34253 ; TGAM_1751 .
    GeneIDi 7987470.
    KEGGi tga:TGAM_1751.

    Phylogenomic databases

    eggNOGi COG1850.
    HOGENOMi HOG000230831.
    KOi K01601.
    OMAi VIVTFRV.

    Enzyme and pathway databases

    BioCyci TGAM593117:GHFT-1782-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    HAMAPi MF_01133. RuBisCO_L_type3.
    InterProi IPR017712. RuBisCO_III.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view ]
    Pfami PF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsi TIGR03326. rubisco_III. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome analysis and genome-wide proteomics of Thermococcus gammatolerans, the most radioresistant organism known amongst the Archaea."
      Zivanovic Y., Armengaud J., Lagorce A., Leplat C., Guerin P., Dutertre M., Anthouard V., Forterre P., Wincker P., Confalonieri F.
      Genome Biol. 10:R70.1-R70.23(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 15229 / JCM 11827 / EJ3Imported.

    Entry informationi

    Entry nameiC5A1I4_THEGJ
    AccessioniPrimary (citable) accession number: C5A1I4
    Entry historyi
    Integrated into UniProtKB/TrEMBL: July 28, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Miscellaneous

    Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3