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C4ZZX9

- TYSY_ECOBW

UniProt

C4ZZX9 - TYSY_ECOBW

Protein

Thymidylate synthase

Gene

thyA

Organism
Escherichia coli (strain K12 / MC4100 / BW2952)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 38 (01 Oct 2014)
      Sequence version 1 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Provides the sole de novo source of dTMP for DNA biosynthesis.UniRule annotation

    Catalytic activityi

    5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei146 – 1461UniRule annotation

    GO - Molecular functioni

    1. thymidylate synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. dTMP biosynthetic process Source: UniProtKB-HAMAP
    2. dTTP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis

    Enzyme and pathway databases

    BioCyciECOL595496:GI18-2663-MONOMER.
    UniPathwayiUPA00575.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
    Short name:
    TSUniRule annotation
    Short name:
    TSaseUniRule annotation
    Gene namesi
    Name:thyAUniRule annotation
    Ordered Locus Names:BWG_2562
    OrganismiEscherichia coli (strain K12 / MC4100 / BW2952)
    Taxonomic identifieri595496 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000001478: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 264264Thymidylate synthasePRO_1000201717Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi595496.BWG_2562.

    Structurei

    3D structure databases

    ProteinModelPortaliC4ZZX9.
    SMRiC4ZZX9. Positions 2-264.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0207.
    HOGENOMiHOG000257900.
    KOiK00560.
    OMAiWRSWATA.
    OrthoDBiEOG6K6V53.

    Family and domain databases

    Gene3Di3.30.572.10. 1 hit.
    HAMAPiMF_00008. Thymidy_synth_bact.
    InterProiIPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view]
    PfamiPF00303. Thymidylat_synt. 1 hit.
    [Graphical view]
    PRINTSiPR00108. THYMDSNTHASE.
    SUPFAMiSSF55831. SSF55831. 1 hit.
    TIGRFAMsiTIGR03284. thym_sym. 2 hits.
    PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C4ZZX9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKQYLELMQK VLDEGTQKND RTGTGTLSIF GHQMRFNLQD GFPLVTTKRC    50
    HLRSIIHELL WFLQGDTNIA YLHENNVTIW DEWADENGDL GPVYGKQWRA 100
    WPTPDGRHID QITTVLNQLK NDPDSRRIIV SAWNVGELDK MALAPCHAFF 150
    QFYVADGKLS CQLYQRSCDV FLGLPFNIAS YALLVHMMAQ QCDLEVGDFV 200
    WTGGDTHLYS NHMDQTHLQL SREPRPLPKL IIKRKPESIF DYRFEDFEIE 250
    GYDPHPGIKA PVAI 264
    Length:264
    Mass (Da):30,480
    Last modified:July 28, 2009 - v1
    Checksum:i0E6D88ED98D24D22
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001396 Genomic DNA. Translation: ACR65659.1.
    RefSeqiYP_002927758.1. NC_012759.1.

    Genome annotation databases

    EnsemblBacteriaiACR65659; ACR65659; BWG_2562.
    GeneIDi7952860.
    KEGGiebw:BWG_2562.
    PATRICi18274610. VBIEscCol60876_2808.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001396 Genomic DNA. Translation: ACR65659.1 .
    RefSeqi YP_002927758.1. NC_012759.1.

    3D structure databases

    ProteinModelPortali C4ZZX9.
    SMRi C4ZZX9. Positions 2-264.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 595496.BWG_2562.

    Chemistry

    BindingDBi C4ZZX9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACR65659 ; ACR65659 ; BWG_2562 .
    GeneIDi 7952860.
    KEGGi ebw:BWG_2562.
    PATRICi 18274610. VBIEscCol60876_2808.

    Phylogenomic databases

    eggNOGi COG0207.
    HOGENOMi HOG000257900.
    KOi K00560.
    OMAi WRSWATA.
    OrthoDBi EOG6K6V53.

    Enzyme and pathway databases

    UniPathwayi UPA00575 .
    BioCyci ECOL595496:GI18-2663-MONOMER.

    Family and domain databases

    Gene3Di 3.30.572.10. 1 hit.
    HAMAPi MF_00008. Thymidy_synth_bact.
    InterProi IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view ]
    Pfami PF00303. Thymidylat_synt. 1 hit.
    [Graphical view ]
    PRINTSi PR00108. THYMDSNTHASE.
    SUPFAMi SSF55831. SSF55831. 1 hit.
    TIGRFAMsi TIGR03284. thym_sym. 2 hits.
    PROSITEi PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic sequencing reveals regulatory mutations and recombinational events in the widely used MC4100 lineage of Escherichia coli K-12."
      Ferenci T., Zhou Z., Betteridge T., Ren Y., Liu Y., Feng L., Reeves P.R., Wang L.
      J. Bacteriol. 191:4025-4029(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MC4100 / BW2952.

    Entry informationi

    Entry nameiTYSY_ECOBW
    AccessioniPrimary (citable) accession number: C4ZZX9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 38 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3