Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphoadenosine phosphosulfate reductase

Gene

cysH

Organism
Escherichia coli (strain K12 / MC4100 / BW2952)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Reduction of activated sulfate into sulfite.UniRule annotation

Catalytic activityi

Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin.UniRule annotation

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes sulfite from sulfate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase subunit 2 (cysD), Sulfate adenylyltransferase subunit 1 (cysN)
  2. Adenylyl-sulfate kinase (cysC)
  3. Phosphoadenosine phosphosulfate reductase (cysH)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Enzyme and pathway databases

UniPathwayiUPA00140; UER00206.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoadenosine phosphosulfate reductaseUniRule annotation (EC:1.8.4.8UniRule annotation)
Alternative name(s):
3'-phosphoadenylylsulfate reductaseUniRule annotation
PAPS reductase, thioredoxin dependentUniRule annotation
PAPS sulfotransferaseUniRule annotation
PAdoPS reductaseUniRule annotation
Gene namesi
Name:cysHUniRule annotation
Ordered Locus Names:BWG_2498
OrganismiEscherichia coli (strain K12 / MC4100 / BW2952)
Taxonomic identifieri595496 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002023951 – 244Phosphoadenosine phosphosulfate reductaseAdd BLAST244

Proteomic databases

PRIDEiC4ZZR6.

Structurei

3D structure databases

ProteinModelPortaliC4ZZR6.
SMRiC4ZZR6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PAPS reductase family. CysH subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000249396.
KOiK00390.
OMAiPRNKLLD.

Family and domain databases

CDDicd01713. PAPS_reductase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00063. CysH. 1 hit.
InterProiIPR004511. PAPS/APS_Rdtase.
IPR002500. PAPS_reduct.
IPR011800. PAPS_reductase_CysH.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01507. PAPS_reduct. 1 hit.
[Graphical view]
PIRSFiPIRSF000857. PAPS_reductase. 1 hit.
TIGRFAMsiTIGR00434. cysH. 1 hit.
TIGR02057. PAPS_reductase. 1 hit.

Sequencei

Sequence statusi: Complete.

C4ZZR6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLDLNALN ELPKVDRILA LAETNAELEK LDAEGRVAWA LDNLPGEYVL
60 70 80 90 100
SSSFGIQAAV SLHLVNQIRP DIPVILTDTG YLFPETYRFI DELTDKLKLN
110 120 130 140 150
LKVYRATESA AWQEARYGKL WEQGVEGIEK YNDINKVEPM NRALKELNAQ
160 170 180 190 200
TWFAGLRREQ SGSRANLPVL AIQRGVFKVL PIIDWDNRTI YQYLQKHGLK
210 220 230 240
YHPLWDEGYL SVGDTHTTRK WEPGMAEEET RFFGLKRECG LHEG
Length:244
Mass (Da):27,976
Last modified:July 28, 2009 - v1
Checksum:i7EC494ED2437E469
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001396 Genomic DNA. Translation: ACR61833.1.
RefSeqiWP_000039850.1. NC_012759.1.

Genome annotation databases

EnsemblBacteriaiACR61833; ACR61833; BWG_2498.
KEGGiebw:BWG_2498.
PATRICi18274466. VBIEscCol60876_2739.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001396 Genomic DNA. Translation: ACR61833.1.
RefSeqiWP_000039850.1. NC_012759.1.

3D structure databases

ProteinModelPortaliC4ZZR6.
SMRiC4ZZR6.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiC4ZZR6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACR61833; ACR61833; BWG_2498.
KEGGiebw:BWG_2498.
PATRICi18274466. VBIEscCol60876_2739.

Phylogenomic databases

HOGENOMiHOG000249396.
KOiK00390.
OMAiPRNKLLD.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00206.

Family and domain databases

CDDicd01713. PAPS_reductase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00063. CysH. 1 hit.
InterProiIPR004511. PAPS/APS_Rdtase.
IPR002500. PAPS_reduct.
IPR011800. PAPS_reductase_CysH.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01507. PAPS_reduct. 1 hit.
[Graphical view]
PIRSFiPIRSF000857. PAPS_reductase. 1 hit.
TIGRFAMsiTIGR00434. cysH. 1 hit.
TIGR02057. PAPS_reductase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCYSH_ECOBW
AccessioniPrimary (citable) accession number: C4ZZR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: November 2, 2016
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.