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Protein

Sulfate adenylyltransferase subunit 2

Gene

cysD

Organism
Escherichia coli (strain K12 / MC4100 / BW2952)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + sulfate = diphosphate + adenylyl sulfate.UniRule annotation

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sulfite from sulfate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase subunit 2 (cysD), Sulfate adenylyltransferase subunit 1 (cysN)
  2. Adenylyl-sulfate kinase (cysC)
  3. Phosphoadenosine phosphosulfate reductase (cysH)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL595496:GI18-2584-MONOMER.
UniPathwayiUPA00140; UER00204.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfate adenylyltransferase subunit 2UniRule annotation (EC:2.7.7.4UniRule annotation)
Alternative name(s):
ATP-sulfurylase small subunitUniRule annotation
Sulfate adenylate transferaseUniRule annotation
Short name:
SATUniRule annotation
Gene namesi
Name:cysDUniRule annotation
Ordered Locus Names:BWG_2488
OrganismiEscherichia coli (strain K12 / MC4100 / BW2952)
Taxonomic identifieri595496 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302Sulfate adenylyltransferase subunit 2PRO_1000202400Add
BLAST

Interactioni

Subunit structurei

Heterodimer composed of CysD, the smaller subunit, and CysN.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliC4ZZQ6.
SMRiC4ZZQ6. Positions 5-212.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PAPS reductase family. CysD subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000263604.
KOiK00957.
OMAiMHVDTTW.
OrthoDBiEOG6W45TZ.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
HAMAPiMF_00064. Sulf_adenylyltr_sub2.
InterProiIPR002500. PAPS_reduct.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011784. SO4_adenylTrfase_ssu.
[Graphical view]
PfamiPF01507. PAPS_reduct. 1 hit.
[Graphical view]
PIRSFiPIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMsiTIGR02039. CysD. 1 hit.

Sequencei

Sequence statusi: Complete.

C4ZZQ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQIRLTHLR QLEAESIHII REVAAEFSNP VMLYSIGKDS SVMLHLARKA
60 70 80 90 100
FYPGTLPFPL LHVDTGWKFR EMYEFRDRTA KAYGCELLVH KNPEGVAMGI
110 120 130 140 150
NPFVHGSAKH TDIMKTEGLK QALNKYGFDA AFGGARRDEE KSRAKERIYS
160 170 180 190 200
FRDRFHRWDP KNQRPELWHN YNGQINKGES IRVFPLSNWT EQDIWQYIWL
210 220 230 240 250
ENIDIVPLYL AAERPVLERD GMLMMIDDNR IDLQPGEVIK KRMVRFRTLG
260 270 280 290 300
CWPLTGAVES NAQTLPEIIE EMLVSTTSER QGRVIDRDQA GSMELKKRQG

YF
Length:302
Mass (Da):35,188
Last modified:July 28, 2009 - v1
Checksum:iA48881C0E5E086A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001396 Genomic DNA. Translation: ACR65597.1.
RefSeqiWP_000372108.1. NC_012759.1.

Genome annotation databases

EnsemblBacteriaiACR65597; ACR65597; BWG_2488.
KEGGiebw:BWG_2488.
PATRICi18274444. VBIEscCol60876_2728.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001396 Genomic DNA. Translation: ACR65597.1.
RefSeqiWP_000372108.1. NC_012759.1.

3D structure databases

ProteinModelPortaliC4ZZQ6.
SMRiC4ZZQ6. Positions 5-212.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACR65597; ACR65597; BWG_2488.
KEGGiebw:BWG_2488.
PATRICi18274444. VBIEscCol60876_2728.

Phylogenomic databases

HOGENOMiHOG000263604.
KOiK00957.
OMAiMHVDTTW.
OrthoDBiEOG6W45TZ.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00204.
BioCyciECOL595496:GI18-2584-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
HAMAPiMF_00064. Sulf_adenylyltr_sub2.
InterProiIPR002500. PAPS_reduct.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011784. SO4_adenylTrfase_ssu.
[Graphical view]
PfamiPF01507. PAPS_reduct. 1 hit.
[Graphical view]
PIRSFiPIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMsiTIGR02039. CysD. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genomic sequencing reveals regulatory mutations and recombinational events in the widely used MC4100 lineage of Escherichia coli K-12."
    Ferenci T., Zhou Z., Betteridge T., Ren Y., Liu Y., Feng L., Reeves P.R., Wang L.
    J. Bacteriol. 191:4025-4029(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MC4100 / BW2952.

Entry informationi

Entry nameiCYSD_ECOBW
AccessioniPrimary (citable) accession number: C4ZZQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: November 11, 2015
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.