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Protein

Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase

Gene

gppA

Organism
Escherichia coli (strain K12 / MC4100 / BW2952)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Conversion of pppGpp to ppGpp. Guanosine pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which together with ppGpp controls the "stringent response", an adaptive process that allows bacteria to respond to amino acid starvation, resulting in the coordinated regulation of numerous cellular activities.UniRule annotation

Catalytic activityi

Guanosine 5'-triphosphate,3'-diphosphate + H2O = guanosine 3',5'-bis(diphosphate) + phosphate.UniRule annotation

Pathway:ippGpp biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes ppGpp from GTP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA)
  2. no protein annotated in this organism
This subpathway is part of the pathway ppGpp biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ppGpp from GTP, the pathway ppGpp biosynthesis and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciECOL595496:GI18-3599-MONOMER.
UniPathwayiUPA00908; UER00884.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanosine-5'-triphosphate,3'-diphosphate pyrophosphataseUniRule annotation (EC:3.6.1.40UniRule annotation)
Alternative name(s):
Guanosine pentaphosphate phosphohydrolaseUniRule annotation
pppGpp-5'-phosphohydrolaseUniRule annotation
Gene namesi
Name:gppAUniRule annotation
Ordered Locus Names:BWG_3462
OrganismiEscherichia coli (strain K12 / MC4100 / BW2952)
Taxonomic identifieri595496 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatasePRO_1000215465Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliC4ZZ47.
SMRiC4ZZ47. Positions 8-493.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GppA/Ppx family. GppA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0248.
HOGENOMiHOG000258672.
KOiK01524.
OMAiFKRAPQH.
OrthoDBiEOG6M6JN6.

Family and domain databases

HAMAPiMF_01550. GppA.
InterProiIPR023709. Guo-5TP_3DP_PyrP.
IPR003695. Ppx_GppA.
IPR030673. PyroPPase_GppA_Ppx.
[Graphical view]
PfamiPF02541. Ppx-GppA. 1 hit.
[Graphical view]
PIRSFiPIRSF001267. Pyrophosphatase_GppA_Ppx. 1 hit.

Sequencei

Sequence statusi: Complete.

C4ZZ47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSTSSLYAA IDLGSNSFHM LVVREVAGSI QTLTRIKRKV RLAAGLNSEN
60 70 80 90 100
ALSNEAMERG WQCLRLFAER LQDIPPSQIR VVATATLRLA VNAGDFIAKA
110 120 130 140 150
QEILGCPVQV ISGEEEARLI YQGVAHTTGG ADQRLVVDIG GASTELVTGT
160 170 180 190 200
GAQTTSLFSL SMGCVTWLER YFADRNLGQE NFDAAEKAAR EVLRPVADEL
210 220 230 240 250
RYHGWKVCVG ASGTVQALQE IMMAQGMDER ITLEKLQQLK QRAIHCGRLE
260 270 280 290 300
ELEIDGLTLE RALVFPSGLA ILIAIFTELN IQCMTLAGGA LREGLVYGML
310 320 330 340 350
HLAVEQDIRS RTLRNIQRRF MIDIDQAQRV AKVAANFFDQ VENEWHLEAI
360 370 380 390 400
SRDLLISACQ LHEIGLSVDF KQAPQHAAYL VRNLDLPGFT PAQKKLLATL
410 420 430 440 450
LLNQTNPVDL SSLHQQNAVP PRVAEQLCRL LRLAIIFASR RRDDLVPEMT
460 470 480 490
LQANHELLTL TLPQGWLTQH PLGKEIIAQE SQWQSYVHWP LEVH
Length:494
Mass (Da):54,871
Last modified:July 28, 2009 - v1
Checksum:iC4DD8DC59432A5F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001396 Genomic DNA. Translation: ACR63395.1.
RefSeqiWP_001295254.1. NC_012759.1.

Genome annotation databases

EnsemblBacteriaiACR63395; ACR63395; BWG_3462.
KEGGiebw:BWG_3462.
PATRICi18276592. VBIEscCol60876_3779.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001396 Genomic DNA. Translation: ACR63395.1.
RefSeqiWP_001295254.1. NC_012759.1.

3D structure databases

ProteinModelPortaliC4ZZ47.
SMRiC4ZZ47. Positions 8-493.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACR63395; ACR63395; BWG_3462.
KEGGiebw:BWG_3462.
PATRICi18276592. VBIEscCol60876_3779.

Phylogenomic databases

eggNOGiCOG0248.
HOGENOMiHOG000258672.
KOiK01524.
OMAiFKRAPQH.
OrthoDBiEOG6M6JN6.

Enzyme and pathway databases

UniPathwayiUPA00908; UER00884.
BioCyciECOL595496:GI18-3599-MONOMER.

Family and domain databases

HAMAPiMF_01550. GppA.
InterProiIPR023709. Guo-5TP_3DP_PyrP.
IPR003695. Ppx_GppA.
IPR030673. PyroPPase_GppA_Ppx.
[Graphical view]
PfamiPF02541. Ppx-GppA. 1 hit.
[Graphical view]
PIRSFiPIRSF001267. Pyrophosphatase_GppA_Ppx. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genomic sequencing reveals regulatory mutations and recombinational events in the widely used MC4100 lineage of Escherichia coli K-12."
    Ferenci T., Zhou Z., Betteridge T., Ren Y., Liu Y., Feng L., Reeves P.R., Wang L.
    J. Bacteriol. 191:4025-4029(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MC4100 / BW2952.

Entry informationi

Entry nameiGPPA_ECOBW
AccessioniPrimary (citable) accession number: C4ZZ47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: July 22, 2015
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.