C4ZYW9 (DGOD_ECOBW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-galactonate dehydratase
Short name=GalD
EC=4.2.1.6

Gene names

Name:	dgoD
Ordered Locus Names:	BWG_3382

Organism

Escherichia coli (strain K12 / MC4100 / BW2952) [Complete proteome] [HAMAP]

Taxonomic identifier

595496 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli (strain K12)

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the dehydration of D-galactonate to 2-keto-3-deoxy-D-galactonate By similarity. HAMAP-Rule MF_01289
Catalytic activity	D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H2O. HAMAP-Rule MF_01289
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Carbohydrate acid metabolism; D-galactonate degradation; D-glyceraldehyde 3-phosphate and pyruvate from D-galactonate: step 1/3. HAMAP-Rule MF_01289
Miscellaneous	Reaction proceeds via an anti dehydration By similarity. HAMAP-Rule MF_01289
Sequence similarities	Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	D-galactonate catabolic process Inferred from electronic annotation. Source: HAMAP cellular amino acid catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	galactonate dehydratase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	D-galactonate dehydratase HAMAP-Rule MF_01289		PRO_1000214215
Sites
	Active site	185	1	Proton donor By similarity
	Active site	285	1	Proton acceptor By similarity
	Metal binding	183	1	Magnesium By similarity
	Metal binding	209	1	Magnesium By similarity
	Metal binding	235	1	Magnesium By similarity
	Site	258	1	Increases basicity of active site His By similarity
	Site	310	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	C4ZYW9 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: B38D4E0B95FD7041	FASTA	382	42,523
	10 20 30 40 50 60 MKITKITTYR LPPRWMFLKI ETDEGVVGWG EPVIEGRART VEAAVHELGD YLIGQDPSRI 70 80 90 100 110 120 NDLWQVMYRA GFYRGGPILM SAIAGIDQAL WDIKGKVLNA PVWQLMGGLV RDKIKAYSWV 130 140 150 160 170 180 GGDRPADVID GIKTLREIGF DTFKLNGCEE LGLIDNSRAV DAAVNTVAQI REAFGNQIEF 190 200 210 220 230 240 GLDFHGRVSA PMAKVLIKEL EPYRPLFIEE PVLAEQAEYY PKLAAQTHIP LAAGERMFSR 250 260 270 280 290 300 FDFKRVLEAG GISILQPDLS HAGGITECYK IAGMAEAYDV TLAPHCPLGP IALAACLHID 310 320 330 340 350 360 FVSYNAVLQE QSMGIHYNKG AELLDFVKNK EDFSMVGGFF KPLTKPGLGV EIDEAKVIEF 370 380 SKNAPDWRNP LWRHEDNSVA EW

References

[1]

"Genomic sequencing reveals regulatory mutations and recombinational events in the widely used MC4100 lineage of Escherichia coli K-12." Ferenci T., Zhou Z., Betteridge T., Ren Y., Liu Y., Feng L., Reeves P.R., Wang L. J. Bacteriol. 191:4025-4029(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MC4100 / BW2952.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001396 Genomic DNA. Translation: ACR63268.1.
RefSeq	YP_002928578.1. NC_012759.1.
3D structure databases
ProteinModelPortal	C4ZYW9.
SMR	C4ZYW9. Positions 1-382.
ModBase	Search...
Protein-protein interaction databases
STRING	595496.BWG_3382.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACR63268; ACR63268; BWG_3382.
GeneID	7953242.
KEGG	ebw:BWG_3382.
PATRIC	18276416. VBIEscCol60876_3697.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4948.
HOGENOM	HOG000113756.
KO	K01684.
OMA	RSAFGNT.
ProtClustDB	PRK14017.
Enzyme and pathway databases
BioCyc	ECOL595496:GI18-1726-MONOMER.
UniPathway	UPA00081; UER00518.
Family and domain databases
HAMAP	MF_01289. Galacton_dehydrat.
InterPro	IPR023592. Galactonate_deHydtase. IPR018110. Mandel_Rmase/mucon_lact_enz_CS. IPR013342. Mandelate_racemase_C. IPR013341. Mandelate_racemase_N. IPR001354. MR_MLE. [Graphical view]
PANTHER	PTHR13794. PTHR13794. 1 hit.
Pfam	PF02746. MR_MLE_N. 1 hit. [Graphical view]
SMART	SM00922. MR_MLE. 1 hit. [Graphical view]
PROSITE	PS00908. MR_MLE_1. 1 hit. PS00909. MR_MLE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DGOD_ECOBW

Accession

Primary (citable) accession number: C4ZYW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 28, 2009
Last modified:	May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program