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Protein

Protein GrpE

Gene

grpE

Organism
Escherichia coli (strain K12 / MC4100 / BW2952)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Names & Taxonomyi

Protein namesi
Recommended name:
Protein GrpEUniRule annotation
Alternative name(s):
HSP-70 cofactorUniRule annotation
Gene namesi
Name:grpEUniRule annotation
Ordered Locus Names:BWG_2372
OrganismiEscherichia coli (strain K12 / MC4100 / BW2952)
Taxonomic identifieri595496 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002136661 – 197Protein GrpEAdd BLAST197

Proteomic databases

PRIDEiC4ZYN1.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
dmsDP698534EBI-4407105,EBI-4406374From a different organism.

Protein-protein interaction databases

IntActiC4ZYN1. 1 interactor.

Structurei

3D structure databases

ProteinModelPortaliC4ZYN1.
SMRiC4ZYN1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GrpE family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252084.
KOiK03687.
OMAiFANENIM.

Family and domain databases

CDDicd00446. GrpE. 1 hit.
Gene3Di2.30.22.10. 1 hit.
3.90.20.20. 1 hit.
HAMAPiMF_01151. GrpE. 1 hit.
InterProiIPR000740. GrpE.
IPR013805. GrpE_coiled_coil.
IPR009012. GrpE_head.
[Graphical view]
PANTHERiPTHR21237. PTHR21237. 1 hit.
PfamiPF01025. GrpE. 1 hit.
[Graphical view]
PRINTSiPR00773. GRPEPROTEIN.
SUPFAMiSSF51064. SSF51064. 1 hit.
PROSITEiPS01071. GRPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C4ZYN1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKVANLEAQ
60 70 80 90 100
LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID
110 120 130 140 150
SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD
160 170 180 190
PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA
Length:197
Mass (Da):21,798
Last modified:July 28, 2009 - v1
Checksum:iCDC4CD9D08AD4BEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001396 Genomic DNA. Translation: ACR63401.1.
RefSeqiWP_001393454.1. NC_012759.1.

Genome annotation databases

EnsemblBacteriaiACR63401; ACR63401; BWG_2372.
KEGGiebw:BWG_2372.
PATRICi18274158. VBIEscCol60876_2592.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001396 Genomic DNA. Translation: ACR63401.1.
RefSeqiWP_001393454.1. NC_012759.1.

3D structure databases

ProteinModelPortaliC4ZYN1.
SMRiC4ZYN1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiC4ZYN1. 1 interactor.

Proteomic databases

PRIDEiC4ZYN1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACR63401; ACR63401; BWG_2372.
KEGGiebw:BWG_2372.
PATRICi18274158. VBIEscCol60876_2592.

Phylogenomic databases

HOGENOMiHOG000252084.
KOiK03687.
OMAiFANENIM.

Family and domain databases

CDDicd00446. GrpE. 1 hit.
Gene3Di2.30.22.10. 1 hit.
3.90.20.20. 1 hit.
HAMAPiMF_01151. GrpE. 1 hit.
InterProiIPR000740. GrpE.
IPR013805. GrpE_coiled_coil.
IPR009012. GrpE_head.
[Graphical view]
PANTHERiPTHR21237. PTHR21237. 1 hit.
PfamiPF01025. GrpE. 1 hit.
[Graphical view]
PRINTSiPR00773. GRPEPROTEIN.
SUPFAMiSSF51064. SSF51064. 1 hit.
PROSITEiPS01071. GRPE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRPE_ECOBW
AccessioniPrimary (citable) accession number: C4ZYN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: November 2, 2016
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.