C4ZYF4 (YDIB_ECOBW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Quinate/shikimate dehydrogenase
EC=1.1.1.282

Alternative name(s):
NAD-dependent shikimate 5-dehydrogenase 2

Gene names

Name:	ydiB
Ordered Locus Names:	BWG_1506

Organism

Escherichia coli (strain K12 / MC4100 / BW2952) [Complete proteome] [HAMAP]

Taxonomic identifier

595496 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli (strain K12)

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-quinate + NAD(P)+ = 3-dehydroquinate + NAD(P)H. HAMAP-Rule MF_01578 Shikimate + NAD(P)+ = 3-dehydroshikimate + NAD(P)H. HAMAP-Rule MF_01578
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP-Rule MF_01578
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW chorismate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	nucleotide binding Inferred from electronic annotation. Source: InterPro quinate 3-dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC quinate 3-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC shikimate 3-dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC shikimate 3-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Quinate/shikimate dehydrogenase HAMAP-Rule MF_01578		PRO_1000215614
Regions
	Nucleotide binding	131 – 135	5	NAD By similarity
	Nucleotide binding	155 – 158	4	NAD By similarity
	Nucleotide binding	255 – 259	5	NAD By similarity
Sites
	Active site	71	1	Proton acceptor Potential
	Binding site	107	1	Substrate By similarity
	Binding site	205	1	NAD; via amide nitrogen By similarity
	Binding site	235	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	C4ZYF4 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: C3D1415E03820A5A	FASTA	288	31,228
	10 20 30 40 50 60 MDVTAKYELI GLMAYPIRHS LSPEMQNKAL EKAGLPFTYM AFEVDNDSFP GAIEGLKALK 70 80 90 100 110 120 MRGTGVSMPN KQLACEYVDE LTPAAKLVGA INTIVNDDGY LRGYNTDGTG HIRAIKESGF 130 140 150 160 170 180 DIKGKTMVLL GAGGASTAIG AQGAIEGLKE IKLFNRRDEF FDKALAFAQR VNENTDCVVT 190 200 210 220 230 240 VTDLADQQAF AEALASADIL TNGTKVGMKP LENESLVNDI SLLHPGLLVT ECVYNPHMTK 250 260 270 280 LLQQAQQAGC KTIDGYGMLL WQGAEQFTLW TGKDFPLEYV KQVMGFGA

References

[1]

"Genomic sequencing reveals regulatory mutations and recombinational events in the widely used MC4100 lineage of Escherichia coli K-12." Ferenci T., Zhou Z., Betteridge T., Ren Y., Liu Y., Feng L., Reeves P.R., Wang L. J. Bacteriol. 191:4025-4029(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MC4100 / BW2952.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001396 Genomic DNA. Translation: ACR65393.1.
RefSeq	YP_002926703.1. NC_012759.1.
3D structure databases
ProteinModelPortal	C4ZYF4.
SMR	C4ZYF4. Positions 5-288.
ModBase	Search...
Protein-protein interaction databases
STRING	595496.BWG_1506.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACR65393; ACR65393; BWG_1506.
GeneID	7955690.
KEGG	ebw:BWG_1506.
PATRIC	18272239. VBIEscCol60876_1652.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0169.
HOGENOM	HOG000237875.
KO	K05887.
OMA	TAIEIQA.
ProtClustDB	PRK12749.
Enzyme and pathway databases
BioCyc	ECOL595496:GI18-3852-MONOMER.
UniPathway	UPA00053; UER00087.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
HAMAP	MF_01578. Shikimate_DH_YdiB.
InterPro	IPR016040. NAD(P)-bd_dom. IPR022872. Quinate/Shikimate_DH. IPR013708. Shikimate_DH-bd_N. IPR022893. Shikimate_quinate_DH. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YDIB_ECOBW

Accession

Primary (citable) accession number: C4ZYF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 28, 2009
Last modified:	May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program