Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

L-threonine 3-dehydrogenase

Gene

tdh

Organism
Escherichia coli (strain K12 / MC4100 / BW2952)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NAD+-dependent oxidation of L-threonine to 2-amino-3-ketobutyrate.UniRule annotation

Catalytic activityi

L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Pathwayi: L-threonine degradation via oxydo-reductase pathway

This protein is involved in step 1 of the subpathway that synthesizes glycine from L-threonine.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. L-threonine 3-dehydrogenase (tdh)
  2. no protein annotated in this organism
This subpathway is part of the pathway L-threonine degradation via oxydo-reductase pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycine from L-threonine, the pathway L-threonine degradation via oxydo-reductase pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi38 – 381Zinc 1; catalyticUniRule annotation
Active sitei40 – 401Charge relay systemUniRule annotation
Active sitei43 – 431Charge relay systemUniRule annotation
Metal bindingi63 – 631Zinc 1; via tele nitrogen; catalyticUniRule annotation
Metal bindingi64 – 641Zinc 1; catalyticUniRule annotation
Metal bindingi93 – 931Zinc 2UniRule annotation
Metal bindingi96 – 961Zinc 2UniRule annotation
Metal bindingi99 – 991Zinc 2UniRule annotation
Metal bindingi107 – 1071Zinc 2UniRule annotation
Sitei148 – 1481Important for catalytic activity for the proton relay mechanism but does not participate directly in the coordination of zinc atomUniRule annotation
Binding sitei175 – 1751NAD; via amide nitrogenUniRule annotation
Binding sitei195 – 1951NADUniRule annotation
Binding sitei200 – 2001NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi262 – 2643NADUniRule annotation
Nucleotide bindingi286 – 2872NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciECOL595496:GI18-3436-MONOMER.
UniPathwayiUPA00046; UER00505.

Names & Taxonomyi

Protein namesi
Recommended name:
L-threonine 3-dehydrogenaseUniRule annotation (EC:1.1.1.103UniRule annotation)
Short name:
TDHUniRule annotation
Gene namesi
Name:tdhUniRule annotation
Ordered Locus Names:BWG_3307
OrganismiEscherichia coli (strain K12 / MC4100 / BW2952)
Taxonomic identifieri595496 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341L-threonine 3-dehydrogenasePRO_1000212311Add
BLAST

Proteomic databases

PRIDEiC4ZXK8.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliC4ZXK8.
SMRiC4ZXK8. Positions 1-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the zinc-containing alcohol dehydrogenase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000294686.
KOiK00060.
OMAiVHPSVTH.
OrthoDBiEOG60PH85.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
HAMAPiMF_00627. Thr_dehydrog.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR004627. L-Threonine_3-DHase.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00692. tdh. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C4ZXK8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKALSKLKAE EGIWMTDVPV PELGHNDLLI KIRKTAICGT DVHIYNWDEW
60 70 80 90 100
SQKTIPVPMV VGHEYVGEVV GIGQEVKGFK IGDRVSGEGH ITCGHCRNCR
110 120 130 140 150
GGRTHLCRNT IGVGVNRPGC FAEYLVIPAF NAFKIPDNIS DDLAAIFDPF
160 170 180 190 200
GNAVHTALSF DLVGEDVLVS GAGPIGIMAA AVAKHVGARN VVITDVNEYR
210 220 230 240 250
LELARKMGIT RAVNVAKENL NDVMAELGMT EGFDVGLEMS GAPPAFRTML
260 270 280 290 300
DTMNHGGRIA MLGIPPSDMS IDWTKVIFKG LFIKGIYGRE MFETWYKMAA
310 320 330 340
LIQSGLDLSP IITHRFSIDD FQKGFDAMRS GQSGKVILSW D
Length:341
Mass (Da):37,239
Last modified:July 28, 2009 - v1
Checksum:i039FBD6B1CE8C2B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001396 Genomic DNA. Translation: ACR65650.1.
RefSeqiWP_000646007.1. NC_012759.1.

Genome annotation databases

EnsemblBacteriaiACR65650; ACR65650; BWG_3307.
KEGGiebw:BWG_3307.
PATRICi18276256. VBIEscCol60876_3618.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001396 Genomic DNA. Translation: ACR65650.1.
RefSeqiWP_000646007.1. NC_012759.1.

3D structure databases

ProteinModelPortaliC4ZXK8.
SMRiC4ZXK8. Positions 1-338.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiC4ZXK8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACR65650; ACR65650; BWG_3307.
KEGGiebw:BWG_3307.
PATRICi18276256. VBIEscCol60876_3618.

Phylogenomic databases

HOGENOMiHOG000294686.
KOiK00060.
OMAiVHPSVTH.
OrthoDBiEOG60PH85.

Enzyme and pathway databases

UniPathwayiUPA00046; UER00505.
BioCyciECOL595496:GI18-3436-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
HAMAPiMF_00627. Thr_dehydrog.
InterProiIPR013149. ADH_C.
IPR013154. ADH_N.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR004627. L-Threonine_3-DHase.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00692. tdh. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genomic sequencing reveals regulatory mutations and recombinational events in the widely used MC4100 lineage of Escherichia coli K-12."
    Ferenci T., Zhou Z., Betteridge T., Ren Y., Liu Y., Feng L., Reeves P.R., Wang L.
    J. Bacteriol. 191:4025-4029(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MC4100 / BW2952.

Entry informationi

Entry nameiTDH_ECOBW
AccessioniPrimary (citable) accession number: C4ZXK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: May 11, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.