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Protein

Trans-aconitate 2-methyltransferase

Gene

tam

Organism
Escherichia coli (strain K12 / MC4100 / BW2952)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + trans-aconitate = S-adenosyl-L-homocysteine + (E)-3-(methoxycarbonyl)pent-2-enedioate.UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciECOL595496:GI18-1383-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Trans-aconitate 2-methyltransferaseUniRule annotation (EC:2.1.1.144UniRule annotation)
Gene namesi
Name:tamUniRule annotation
Ordered Locus Names:BWG_1338
OrganismiEscherichia coli (strain K12 / MC4100 / BW2952)
Taxonomic identifieri595496 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 252252Trans-aconitate 2-methyltransferasePRO_1000212042Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliC4ZWT6.
SMRiC4ZWT6. Positions 9-248.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the methyltransferase superfamily. Tam family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000125295.
KOiK00598.
OMAiARVDVWR.
OrthoDBiEOG6716PV.

Family and domain databases

Gene3Di1.10.150.290. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00560. Tran_acon_Me_trans.
InterProiIPR029063. SAM-dependent_MTases.
IPR023506. Trans-aconitate_MeTrfase.
IPR023149. Trans_acon_MeTrfase_C.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

C4ZWT6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDWNPSLYL HFSAERSRPA VELLARVPLE NVEYVADLGC GPGNSTALLQ
60 70 80 90 100
QRWPAARITG IDSSPAMIAE ARSALPDCQF VEADIRNWQP VQALDLIFAN
110 120 130 140 150
ASLQWLPDHY ELFPHLVSLL NPQGVLAVQM PDNWLEPTHV LMREVAWEQN
160 170 180 190 200
YPDRGREPLA GVHAYYDILS EAGCEVDIWR TTYYHQMPSH QAIIDWVTAT
210 220 230 240 250
GLRPWLQDLT ESEQQLFLKR YHQMLEEQYP LQENGQILLA FPRLFIVARR

ME
Length:252
Mass (Da):29,006
Last modified:July 28, 2009 - v1
Checksum:i917BB2F7F1858B4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001396 Genomic DNA. Translation: ACR65671.1.
RefSeqiWP_001286597.1. NC_012759.1.

Genome annotation databases

EnsemblBacteriaiACR65671; ACR65671; BWG_1338.
KEGGiebw:BWG_1338.
PATRICi18271883. VBIEscCol60876_1476.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001396 Genomic DNA. Translation: ACR65671.1.
RefSeqiWP_001286597.1. NC_012759.1.

3D structure databases

ProteinModelPortaliC4ZWT6.
SMRiC4ZWT6. Positions 9-248.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACR65671; ACR65671; BWG_1338.
KEGGiebw:BWG_1338.
PATRICi18271883. VBIEscCol60876_1476.

Phylogenomic databases

HOGENOMiHOG000125295.
KOiK00598.
OMAiARVDVWR.
OrthoDBiEOG6716PV.

Enzyme and pathway databases

BioCyciECOL595496:GI18-1383-MONOMER.

Family and domain databases

Gene3Di1.10.150.290. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00560. Tran_acon_Me_trans.
InterProiIPR029063. SAM-dependent_MTases.
IPR023506. Trans-aconitate_MeTrfase.
IPR023149. Trans_acon_MeTrfase_C.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genomic sequencing reveals regulatory mutations and recombinational events in the widely used MC4100 lineage of Escherichia coli K-12."
    Ferenci T., Zhou Z., Betteridge T., Ren Y., Liu Y., Feng L., Reeves P.R., Wang L.
    J. Bacteriol. 191:4025-4029(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MC4100 / BW2952.

Entry informationi

Entry nameiTAM_ECOBW
AccessioniPrimary (citable) accession number: C4ZWT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: November 11, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.