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Protein

Octanoyltransferase

Gene

lipB

Organism
Escherichia coli (strain K12 / MC4100 / BW2952)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.UniRule annotation

Miscellaneous

In the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes.UniRule annotation

Catalytic activityi

Octanoyl-[acyl-carrier-protein] + protein = protein N6-(octanoyl)lysine + [acyl-carrier-protein].UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 1 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (lipB)
  2. Lipoyl synthase (lipA)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei135Lowers pKa of active site CysUniRule annotation1
Active sitei169Acyl-thioester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00538; UER00592

Names & Taxonomyi

Protein namesi
Recommended name:
OctanoyltransferaseUniRule annotation (EC:2.3.1.181UniRule annotation)
Alternative name(s):
Lipoate-protein ligase BUniRule annotation
Lipoyl/octanoyl transferaseUniRule annotation
Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferaseUniRule annotation
Gene namesi
Name:lipBUniRule annotation
Ordered Locus Names:BWG_0501
OrganismiEscherichia coli (strain K12 / MC4100 / BW2952)
Taxonomic identifieri595496 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002017951 – 213OctanoyltransferaseAdd BLAST213

Structurei

3D structure databases

ProteinModelPortaliC4ZWB7
SMRiC4ZWB7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 207BPL/LPL catalyticPROSITE-ProRule annotationAdd BLAST176

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni71 – 78Substrate bindingUniRule annotation8
Regioni138 – 140Substrate bindingUniRule annotation3
Regioni151 – 153Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the LipB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000194321
KOiK03801
OMAiMHGFAFN

Family and domain databases

CDDicd16444 LipB, 1 hit
HAMAPiMF_00013 LipB, 1 hit
InterProiView protein in InterPro
IPR004143 BPL_LPL_catalytic
IPR000544 Octanoyltransferase
IPR020605 Octanoyltransferase_CS
PfamiView protein in Pfam
PF03099 BPL_LplA_LipB, 1 hit
PIRSFiPIRSF016262 LPLase, 1 hit
TIGRFAMsiTIGR00214 lipB, 1 hit
PROSITEiView protein in PROSITE
PS51733 BPL_LPL_CATALYTIC, 1 hit
PS01313 LIPB, 1 hit

Sequencei

Sequence statusi: Complete.

C4ZWB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYQDKILVRQ LGLQPYEPIS QAMHEFTDTR DDSTLDEIWL VEHYPVFTQG
60 70 80 90 100
QAGKAEHILM PGDIPVIQSD RGGQVTYHGP GQQVMYVLLN LKRRKLGVRE
110 120 130 140 150
LVTLLEQTVV NTLAELGIEA HPRADAPGVY VGEKKICSLG LRIRRGCSFH
160 170 180 190 200
GLALNVNMDL SPFLRINPCG YAGMEMAKIS QWKPEATTNN IAPRLLENIL
210
ALLNNPDFEY ITA
Length:213
Mass (Da):23,883
Last modified:July 28, 2009 - v1
Checksum:i56EE2BB105F99EAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001396 Genomic DNA Translation: ACR63668.1
RefSeqiWP_000284027.1, NC_012759.1

Genome annotation databases

EnsemblBacteriaiACR63668; ACR63668; BWG_0501
KEGGiebw:BWG_0501

Similar proteinsi

Entry informationi

Entry nameiLIPB_ECOBW
AccessioniPrimary (citable) accession number: C4ZWB7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: February 28, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health