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Protein

Methionine--tRNA ligase

Gene

metG

Organism
Escherichia coli (strain K12 / MC4100 / BW2952)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.UniRule annotation

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi146 – 1461ZincUniRule annotation
Metal bindingi149 – 1491ZincUniRule annotation
Metal bindingi159 – 1591ZincUniRule annotation
Metal bindingi162 – 1621ZincUniRule annotation
Binding sitei336 – 3361ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW
  3. methionine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. methionyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Enzyme and pathway databases

BioCyciECOL595496:GI18-1967-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligaseUniRule annotation (EC:6.1.1.10UniRule annotation)
Alternative name(s):
Methionyl-tRNA synthetaseUniRule annotation
Short name:
MetRSUniRule annotation
Gene namesi
Name:metGUniRule annotation
Ordered Locus Names:BWG_1900
OrganismiEscherichia coli (strain K12 / MC4100 / BW2952)
Taxonomic identifieri595496 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001478 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 677677Methionine--tRNA ligasePRO_1000202756Add
BLAST

Proteomic databases

PRIDEiC4ZSJ8.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi595496.BWG_1900.

Structurei

3D structure databases

ProteinModelPortaliC4ZSJ8.
SMRiC4ZSJ8. Positions 5-548, 571-676.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini575 – 677103tRNA-bindingUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi15 – 2511"HIGH" regionAdd
BLAST
Motifi333 – 3375"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.UniRule annotation
Contains 1 tRNA-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0143.
HOGENOMiHOG000200400.
KOiK01874.
OMAiECLRYYY.
OrthoDBiEOG6CVV9B.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
2.20.28.20. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_bsu_C.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR029038. MetRS_Zn.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamiPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C4ZSJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQVAKKILV TCALPYANGS IHLGHMLEHI QADVWVRYQR MRGHEVNFIC
60 70 80 90 100
ADDAHGTPIM LKAQQLGITP EQMIGEMSQE HQTDFAGFNI SYDNYHSTHS
110 120 130 140 150
EENRQLSELI YSRLKENGFI KNRTISQLYD PEKGMFLPDR FVKGTCPKCK
160 170 180 190 200
SPDQYGDNCE VCGATYSPTE LIEPKSVVSG ATPVMRDSEH FFFDLPSFSE
210 220 230 240 250
MLQAWTRSGA LQEQVANKMQ EWFESGLQQW DISRDAPYFG FEIPNAPGKY
260 270 280 290 300
FYVWLDAPIG YMGSFKNLCD KRGDSVSFDE YWKKDSTAEL YHFIGKDIVY
310 320 330 340 350
FHSLFWPAML EGSNFRKPSN LFVHGYVTVN GAKMSKSRGT FIKASTWLNH
360 370 380 390 400
FDADSLRYYY TAKLSSRIDD IDLNLEDFVQ RVNADIVNKV VNLASRNAGF
410 420 430 440 450
INKRFDGVLA SELADPQLYK TFTDAAEVIG EAWESREFGK AVREIMALAD
460 470 480 490 500
LANRYVDEQA PWVVAKQEGR DADLQAICSM GINLFRVLMT YLKPVLPKLT
510 520 530 540 550
ERAEAFLNTE LTWDGIQQPL LGHKVNPFKA LYNRIDMRQV EALVEASKEE
560 570 580 590 600
VKAAAAPVTG PLADDPIQET ITFDDFAKVD LRVALIENAE FVEGSDKLLR
610 620 630 640 650
LTLDLGGEKR NVFSGIRSAY PDPQALIGRH TIMVANLAPR KMRFGISEGM
660 670
VMAAGPGGKD IFLLSPDAGA KPGHQVK
Length:677
Mass (Da):76,255
Last modified:July 28, 2009 - v1
Checksum:i363F3324AFD3202C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001396 Genomic DNA. Translation: ACR63756.1.
RefSeqiYP_002927096.1. NC_012759.1.

Genome annotation databases

EnsemblBacteriaiACR63756; ACR63756; BWG_1900.
KEGGiebw:BWG_1900.
PATRICi18273113. VBIEscCol60876_2082.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001396 Genomic DNA. Translation: ACR63756.1.
RefSeqiYP_002927096.1. NC_012759.1.

3D structure databases

ProteinModelPortaliC4ZSJ8.
SMRiC4ZSJ8. Positions 5-548, 571-676.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi595496.BWG_1900.

Chemistry

BindingDBiC4ZSJ8.

Proteomic databases

PRIDEiC4ZSJ8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACR63756; ACR63756; BWG_1900.
KEGGiebw:BWG_1900.
PATRICi18273113. VBIEscCol60876_2082.

Phylogenomic databases

eggNOGiCOG0143.
HOGENOMiHOG000200400.
KOiK01874.
OMAiECLRYYY.
OrthoDBiEOG6CVV9B.

Enzyme and pathway databases

BioCyciECOL595496:GI18-1967-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
2.20.28.20. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00098. Met_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_bsu_C.
IPR023458. Met-tRNA_ligase_1.
IPR014758. Met-tRNA_synth.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR029038. MetRS_Zn.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamiPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF57770. SSF57770. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genomic sequencing reveals regulatory mutations and recombinational events in the widely used MC4100 lineage of Escherichia coli K-12."
    Ferenci T., Zhou Z., Betteridge T., Ren Y., Liu Y., Feng L., Reeves P.R., Wang L.
    J. Bacteriol. 191:4025-4029(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MC4100 / BW2952.

Entry informationi

Entry nameiSYM_ECOBW
AccessioniPrimary (citable) accession number: C4ZSJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: April 1, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.