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C4ZSJ8

- SYM_ECOBW

UniProt

C4ZSJ8 - SYM_ECOBW

Protein

Methionine--tRNA ligase

Gene

metG

Organism
Escherichia coli (strain K12 / MC4100 / BW2952)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.UniRule annotation

    Catalytic activityi

    ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi146 – 1461ZincUniRule annotation
    Metal bindingi149 – 1491ZincUniRule annotation
    Metal bindingi159 – 1591ZincUniRule annotation
    Metal bindingi162 – 1621ZincUniRule annotation
    Binding sitei336 – 3361ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW
    3. methionine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. methionyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

    Enzyme and pathway databases

    BioCyciECOL595496:GI18-1967-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methionine--tRNA ligaseUniRule annotation (EC:6.1.1.10UniRule annotation)
    Alternative name(s):
    Methionyl-tRNA synthetaseUniRule annotation
    Short name:
    MetRSUniRule annotation
    Gene namesi
    Name:metGUniRule annotation
    Ordered Locus Names:BWG_1900
    OrganismiEscherichia coli (strain K12 / MC4100 / BW2952)
    Taxonomic identifieri595496 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000001478: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 677677Methionine--tRNA ligasePRO_1000202756Add
    BLAST

    Proteomic databases

    PRIDEiC4ZSJ8.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi595496.BWG_1900.

    Structurei

    3D structure databases

    ProteinModelPortaliC4ZSJ8.
    SMRiC4ZSJ8. Positions 5-548, 571-676.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini575 – 677103tRNA-bindingUniRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi15 – 2511"HIGH" regionAdd
    BLAST
    Motifi333 – 3375"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.UniRule annotation
    Contains 1 tRNA-binding domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0143.
    HOGENOMiHOG000200400.
    KOiK01874.
    OMAiNQCIYVC.
    OrthoDBiEOG6CVV9B.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    2.20.28.20. 1 hit.
    2.40.50.140. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00098. Met_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR004495. Met-tRNA-synth_Ia_bsu_C.
    IPR023458. Met-tRNA_ligase_1.
    IPR014758. Met-tRNA_synth.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR029038. MetRS_Zn.
    IPR012340. NA-bd_OB-fold.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR002547. tRNA-bd_dom.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view]
    PfamiPF09334. tRNA-synt_1g. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view]
    PRINTSiPR01041. TRNASYNTHMET.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF57770. SSF57770. 1 hit.
    TIGRFAMsiTIGR00398. metG. 1 hit.
    TIGR00399. metG_C_term. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C4ZSJ8-1 [UniParc]FASTAAdd to Basket

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    MTQVAKKILV TCALPYANGS IHLGHMLEHI QADVWVRYQR MRGHEVNFIC    50
    ADDAHGTPIM LKAQQLGITP EQMIGEMSQE HQTDFAGFNI SYDNYHSTHS 100
    EENRQLSELI YSRLKENGFI KNRTISQLYD PEKGMFLPDR FVKGTCPKCK 150
    SPDQYGDNCE VCGATYSPTE LIEPKSVVSG ATPVMRDSEH FFFDLPSFSE 200
    MLQAWTRSGA LQEQVANKMQ EWFESGLQQW DISRDAPYFG FEIPNAPGKY 250
    FYVWLDAPIG YMGSFKNLCD KRGDSVSFDE YWKKDSTAEL YHFIGKDIVY 300
    FHSLFWPAML EGSNFRKPSN LFVHGYVTVN GAKMSKSRGT FIKASTWLNH 350
    FDADSLRYYY TAKLSSRIDD IDLNLEDFVQ RVNADIVNKV VNLASRNAGF 400
    INKRFDGVLA SELADPQLYK TFTDAAEVIG EAWESREFGK AVREIMALAD 450
    LANRYVDEQA PWVVAKQEGR DADLQAICSM GINLFRVLMT YLKPVLPKLT 500
    ERAEAFLNTE LTWDGIQQPL LGHKVNPFKA LYNRIDMRQV EALVEASKEE 550
    VKAAAAPVTG PLADDPIQET ITFDDFAKVD LRVALIENAE FVEGSDKLLR 600
    LTLDLGGEKR NVFSGIRSAY PDPQALIGRH TIMVANLAPR KMRFGISEGM 650
    VMAAGPGGKD IFLLSPDAGA KPGHQVK 677
    Length:677
    Mass (Da):76,255
    Last modified:July 28, 2009 - v1
    Checksum:i363F3324AFD3202C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001396 Genomic DNA. Translation: ACR63756.1.
    RefSeqiYP_002927096.1. NC_012759.1.

    Genome annotation databases

    EnsemblBacteriaiACR63756; ACR63756; BWG_1900.
    GeneIDi7952481.
    KEGGiebw:BWG_1900.
    PATRICi18273113. VBIEscCol60876_2082.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001396 Genomic DNA. Translation: ACR63756.1 .
    RefSeqi YP_002927096.1. NC_012759.1.

    3D structure databases

    ProteinModelPortali C4ZSJ8.
    SMRi C4ZSJ8. Positions 5-548, 571-676.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 595496.BWG_1900.

    Chemistry

    BindingDBi C4ZSJ8.

    Proteomic databases

    PRIDEi C4ZSJ8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACR63756 ; ACR63756 ; BWG_1900 .
    GeneIDi 7952481.
    KEGGi ebw:BWG_1900.
    PATRICi 18273113. VBIEscCol60876_2082.

    Phylogenomic databases

    eggNOGi COG0143.
    HOGENOMi HOG000200400.
    KOi K01874.
    OMAi NQCIYVC.
    OrthoDBi EOG6CVV9B.

    Enzyme and pathway databases

    BioCyci ECOL595496:GI18-1967-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    2.20.28.20. 1 hit.
    2.40.50.140. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00098. Met_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR004495. Met-tRNA-synth_Ia_bsu_C.
    IPR023458. Met-tRNA_ligase_1.
    IPR014758. Met-tRNA_synth.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR029038. MetRS_Zn.
    IPR012340. NA-bd_OB-fold.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR002547. tRNA-bd_dom.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view ]
    Pfami PF09334. tRNA-synt_1g. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view ]
    PRINTSi PR01041. TRNASYNTHMET.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF57770. SSF57770. 1 hit.
    TIGRFAMsi TIGR00398. metG. 1 hit.
    TIGR00399. metG_C_term. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic sequencing reveals regulatory mutations and recombinational events in the widely used MC4100 lineage of Escherichia coli K-12."
      Ferenci T., Zhou Z., Betteridge T., Ren Y., Liu Y., Feng L., Reeves P.R., Wang L.
      J. Bacteriol. 191:4025-4029(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MC4100 / BW2952.

    Entry informationi

    Entry nameiSYM_ECOBW
    AccessioniPrimary (citable) accession number: C4ZSJ8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3