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C4ZSI8

- THIM_ECOBW

UniProt

C4ZSI8 - THIM_ECOBW

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Protein
Hydroxyethylthiazole kinase
Gene
thiM, BWG_1890
Organism
Escherichia coli (strain K12 / MC4100 / BW2952)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501Substrate; via amide nitrogen By similarity
Binding sitei125 – 1251ATP By similarity
Binding sitei171 – 1711ATP By similarity
Binding sitei198 – 1981Substrate; via amide nitrogen By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. hydroxyethylthiazole kinase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL595496:GI18-1957-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinase (EC:2.7.1.50)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name:
TH kinase
Short name:
Thz kinase
Gene namesi
Name:thiM
Ordered Locus Names:BWG_1890
OrganismiEscherichia coli (strain K12 / MC4100 / BW2952)
Taxonomic identifieri595496 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001478: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 262262Hydroxyethylthiazole kinaseUniRule annotation
PRO_1000204359Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi595496.BWG_1890.

Structurei

3D structure databases

ProteinModelPortaliC4ZSI8.
SMRiC4ZSI8. Positions 6-259.

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiQTFTANV.
OrthoDBiEOG628F8M.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

C4ZSI8-1 [UniParc]FASTAAdd to Basket

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MQVDLLGSAQ SAHALHLFHQ HSPLVHCMTN DVVQTFTANT LLALGASPAM    50
VIETEEASQF AAIASALLIN VGTLTQPRAQ AMRAAVEQAK SSQTPWTLDP 100
VAVGALDYRR HFCHELLSFK PAAIRGNASE IMALAGIANG GRGVDTTDAA 150
ANAIPAAQTL ARETGAIVVV TGEMDYVTDG HRIIGIHGGD PLMTKVVGTG 200
CALSAVVAAC CALPGDTLEN VASACHWMKQ AGERAVARSE GPGSFVPHFL 250
DALWQLTQEV QA 262
Length:262
Mass (Da):27,339
Last modified:July 28, 2009 - v1
Checksum:i720774031BA7AC4B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001396 Genomic DNA. Translation: ACR63443.1.
RefSeqiYP_002927086.1. NC_012759.1.

Genome annotation databases

EnsemblBacteriaiACR63443; ACR63443; BWG_1890.
GeneIDi7952570.
KEGGiebw:BWG_1890.
PATRICi18273093. VBIEscCol60876_2072.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001396 Genomic DNA. Translation: ACR63443.1 .
RefSeqi YP_002927086.1. NC_012759.1.

3D structure databases

ProteinModelPortali C4ZSI8.
SMRi C4ZSI8. Positions 6-259.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 595496.BWG_1890.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACR63443 ; ACR63443 ; BWG_1890 .
GeneIDi 7952570.
KEGGi ebw:BWG_1890.
PATRICi 18273093. VBIEscCol60876_2072.

Phylogenomic databases

eggNOGi COG2145.
HOGENOMi HOG000114352.
KOi K00878.
OMAi QTFTANV.
OrthoDBi EOG628F8M.

Enzyme and pathway databases

UniPathwayi UPA00060 ; UER00139 .
BioCyci ECOL595496:GI18-1957-MONOMER.

Family and domain databases

Gene3Di 3.40.1190.20. 1 hit.
HAMAPi MF_00228. Thz_kinase.
InterProi IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view ]
Pfami PF02110. HK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000513. Thz_kinase. 1 hit.
PRINTSi PR01099. HYETHTZKNASE.
SUPFAMi SSF53613. SSF53613. 1 hit.
TIGRFAMsi TIGR00694. thiM. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genomic sequencing reveals regulatory mutations and recombinational events in the widely used MC4100 lineage of Escherichia coli K-12."
    Ferenci T., Zhou Z., Betteridge T., Ren Y., Liu Y., Feng L., Reeves P.R., Wang L.
    J. Bacteriol. 191:4025-4029(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MC4100 / BW2952.

Entry informationi

Entry nameiTHIM_ECOBW
AccessioniPrimary (citable) accession number: C4ZSI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: June 11, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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