C4ZQ57 (PNCB_ECOBW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nicotinate phosphoribosyltransferase
Short name=NAPRTase
EC=2.4.2.11

Gene names

Name:	pncB
Ordered Locus Names:	BWG_0783

Organism

Escherichia coli (strain K12 / MC4100 / BW2952) [Complete proteome] [HAMAP]

Taxonomic identifier

595496 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli (strain K12)

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Beta-nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_00570
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1. HAMAP-Rule MF_00570
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NAPRTase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP nicotinate nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	nicotinate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP nicotinate-nucleotide diphosphorylase (carboxylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Nicotinate phosphoribosyltransferase HAMAP-Rule MF_00570		PRO_1000212083

Sequences

	Sequence		Length	Mass (Da)
	C4ZQ57 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: 1D5FFC61EBAAE1D9	FASTA	400	45,897
	10 20 30 40 50 60 MTQFASPVLH SLLDTDAYKL HMQQAVFHHY YDVHVAAEFR CRGDDLLGIY ADAIREQVQA 70 80 90 100 110 120 MQHLRLQDDE YQWLSALPFF KADYLNWLRE FRFNPEQVTV SNDNGKLDIR LSGPWREVIL 130 140 150 160 170 180 WEVPLLAVIS EMVHRYRSPQ ADVAQALDTL ESKLVDFSAL TAGLDMSRFH LMDFGTRRRF 190 200 210 220 230 240 SREVQETIVK RLQQESWFVG TSNYDLARRL SLTPMGTQAH EWFQAHQQIS PDLANSQRAA 250 260 270 280 290 300 LAAWLEEYPD QLGIALTDCI TMDAFLRDFG VEFASRYQGL RHDSGDPVEW GEKAIAHYEK 310 320 330 340 350 360 LGIDPQSKTL VFSDNLDLRK AVELYRHFSS RVQLSFGIGT RLTCDIPQVK PLNIVIKLVE 370 380 390 400 CNGKPVAKLS DSPGKTICHD KAFVRALRKA FDLPHIKKAS

References

[1]

"Genomic sequencing reveals regulatory mutations and recombinational events in the widely used MC4100 lineage of Escherichia coli K-12." Ferenci T., Zhou Z., Betteridge T., Ren Y., Liu Y., Feng L., Reeves P.R., Wang L. J. Bacteriol. 191:4025-4029(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MC4100 / BW2952.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001396 Genomic DNA. Translation: ACR63892.1.
RefSeq	YP_002925985.1. NC_012759.1.
3D structure databases
ProteinModelPortal	C4ZQ57.
SMR	C4ZQ57. Positions 3-394.
ModBase	Search...
Protein-protein interaction databases
STRING	595496.BWG_0783.
Proteomic databases
PRIDE	C4ZQ57.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACR63892; ACR63892; BWG_0783.
GeneID	7952509.
KEGG	ebw:BWG_0783.
PATRIC	18270649. VBIEscCol60876_0863.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1488.
HOGENOM	HOG000284928.
KO	K00763.
OMA	SFGWGTN.
ProtClustDB	PRK05321.
Enzyme and pathway databases
BioCyc	ECOL595496:GI18-2350-MONOMER.
UniPathway	UPA00253; UER00457.
Family and domain databases
HAMAP	MF_00570. NAPRTase.
InterPro	IPR006406. Nic_PRibTrfase. IPR015977. Nic_PRibTrfase-like. IPR007229. Nic_PRibTrfase-rel. IPR002638. Quinolinate_PRibosylTrfase_C. [Graphical view]
PANTHER	PTHR11098:SF1. PTHR11098:SF1. 1 hit.
Pfam	PF04095. NAPRTase. 1 hit. [Graphical view]
PIRSF	PIRSF000484. NAPRT. 1 hit.
SUPFAM	SSF51690. Q_phspho_trans. 1 hit.
TIGRFAMs	TIGR01514. NAPRTase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PNCB_ECOBW

Accession

Primary (citable) accession number: C4ZQ57

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 28, 2009
Last modified:	May 1, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program