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Protein

LL-diaminopimelate aminotransferase

Gene

dapL

Organism
Agathobacter rectalis (strain ATCC 33656 / DSM 3377 / JCM 17463 / KCTC 5835 / VPI 0990) (Eubacterium rectale)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL-diaminopimelate.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate + 2-oxoglutarate = (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + L-glutamate + H2O.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (aminotransferase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. LL-diaminopimelate aminotransferase (dapL)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (aminotransferase route), the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15SubstrateUniRule annotation1
Binding sitei42Substrate; via amide nitrogenUniRule annotation1
Binding sitei72Pyridoxal phosphateUniRule annotation1
Binding sitei109SubstrateUniRule annotation1
Binding sitei132Pyridoxal phosphateUniRule annotation1
Binding sitei132SubstrateUniRule annotation1
Binding sitei188Pyridoxal phosphateUniRule annotation1
Binding sitei188SubstrateUniRule annotation1
Binding sitei219Pyridoxal phosphateUniRule annotation1
Binding sitei258Pyridoxal phosphateUniRule annotation1
Binding sitei288Pyridoxal phosphateUniRule annotation1
Binding sitei288SubstrateUniRule annotation1
Binding sitei384SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00034; UER00466.

Names & Taxonomyi

Protein namesi
Recommended name:
LL-diaminopimelate aminotransferaseUniRule annotation (EC:2.6.1.83UniRule annotation)
Short name:
DAP-ATUniRule annotation
Short name:
DAP-aminotransferaseUniRule annotation
Short name:
LL-DAP-aminotransferaseUniRule annotation
Gene namesi
Name:dapLUniRule annotation
Ordered Locus Names:EUBREC_2541
OrganismiAgathobacter rectalis (strain ATCC 33656 / DSM 3377 / JCM 17463 / KCTC 5835 / VPI 0990) (Eubacterium rectale)
Taxonomic identifieri515619 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesLachnospiraceae
Proteomesi
  • UP000001477 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002158311 – 404LL-diaminopimelate aminotransferaseAdd BLAST404

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei250N6-(pyridoxal phosphate)lysineUniRule annotation1

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliC4ZG66.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni108 – 109Pyridoxal phosphate bindingUniRule annotation2
Regioni247 – 249Pyridoxal phosphate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. LL-diaminopimelate aminotransferase subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000223061.
KOiK10206.
OMAiRCAFTVV.
OrthoDBiPOG091H0235.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01642. DapL_aminotrans_1. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR019942. DapL_aminotrans.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11751:SF22. PTHR11751:SF22. 1 hit.
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR03542. DAPAT_plant. 1 hit.

Sequencei

Sequence statusi: Complete.

C4ZG66-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFQINDNFQK LPGSYLFSTI AKKVAAYQEA NPDKEIIRLG IGDVTQPLAP
60 70 80 90 100
AIIDALHKAV DEMGNAATFH GYAPDLGYEF LRKAISDNDY KARGCDISAD
110 120 130 140 150
EIFVSDGAKS DSANIQELFS ANSRIAVTDP VYPVYVDSNV MAGRTGTYDA
160 170 180 190 200
QTETWSNVIY MPSTADNGFV PELPKEVPDM IYLCLPNNPT GTTLKKEQLQ
210 220 230 240 250
VWVDYANKNG SVIIFDAAYE AYISEADVPH SIYECNGAKT CAIELRSFSK
260 270 280 290 300
NAGFTGVRLG FTVVPKELKC GDVSLHAMWA RRHGTKFNGA PYIVQRAGEA
310 320 330 340 350
VYSDAGKAQL KDQVAYYMNN AKTIKTGLAE AGFTVYGGVN APYIWLKTPD
360 370 380 390 400
QMTSWEFFDY LLENANVVGT PGSGFGPSGE GYFRLTAFGN YENTVKALER

IKAL
Length:404
Mass (Da):44,267
Last modified:July 28, 2009 - v1
Checksum:i5D68F2871E0BB880
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001107 Genomic DNA. Translation: ACR76272.1.
RefSeqiWP_012743361.1. NC_012781.1.

Genome annotation databases

EnsemblBacteriaiACR76272; ACR76272; EUBREC_2541.
KEGGiere:EUBREC_2541.
PATRICi21873135. VBIEubRec107985_2207.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001107 Genomic DNA. Translation: ACR76272.1.
RefSeqiWP_012743361.1. NC_012781.1.

3D structure databases

ProteinModelPortaliC4ZG66.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACR76272; ACR76272; EUBREC_2541.
KEGGiere:EUBREC_2541.
PATRICi21873135. VBIEubRec107985_2207.

Phylogenomic databases

HOGENOMiHOG000223061.
KOiK10206.
OMAiRCAFTVV.
OrthoDBiPOG091H0235.

Enzyme and pathway databases

UniPathwayiUPA00034; UER00466.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01642. DapL_aminotrans_1. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR019942. DapL_aminotrans.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11751:SF22. PTHR11751:SF22. 1 hit.
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR03542. DAPAT_plant. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDAPAT_AGARV
AccessioniPrimary (citable) accession number: C4ZG66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: November 2, 2016
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.