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Protein

NAD kinase

Gene

nadK

Organism
Eubacterium rectale (strain ATCC 33656 / VPI 0990)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661Proton acceptorUniRule annotation
Binding sitei71 – 711NADUniRule annotation
Binding sitei165 – 1651NADUniRule annotation
Binding sitei167 – 1671NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi66 – 672NADUniRule annotation
Nucleotide bindingi137 – 1382NADUniRule annotation
Nucleotide bindingi178 – 1836NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciEREC515619:GHMX-2198-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:EUBREC_2207
OrganismiEubacterium rectale (strain ATCC 33656 / VPI 0990)
Taxonomic identifieri515619 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesEubacteriaceaeEubacterium
ProteomesiUP000001477 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 283283NAD kinasePRO_1000205416Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi515619.EUBREC_2207.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227222.
KOiK00858.
OMAiLLWGESP.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

C4ZCY4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHFVVIANA YKDRDFALTN KIVAYIEQKG GTAKGLMSNV EAISDNEFEL
60 70 80 90 100
EDIPQDTQCI LVLGGDGTLI RAATRVETLE IPLMGVNLGT LGYLCEVEEA
110 120 130 140 150
TVFDAIDSLM ADKYMTEDRI MLIGHKRGSE TSRVALNDIV IHRKGNLQIL
160 170 180 190 200
SLNVYVNGEF LNNYHADGII VATPTGSTGY SMSAGGPIVD PKGDMILLTP
210 220 230 240 250
NNAHNLTSKS IVLSGDDEIE IEILSRREQN DELACVSYDG DTTAELAVGD
260 270 280
RFVISRAANH TKICKLHQRS FLEILRKKMG NYS
Length:283
Mass (Da):31,056
Last modified:July 28, 2009 - v1
Checksum:i41A340FFF08F45F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001107 Genomic DNA. Translation: ACR75947.1.
RefSeqiWP_012743042.1. NC_012781.1.
YP_002938081.1. NC_012781.1.

Genome annotation databases

EnsemblBacteriaiACR75947; ACR75947; EUBREC_2207.
KEGGiere:EUBREC_2207.
PATRICi21872549. VBIEubRec107985_1921.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001107 Genomic DNA. Translation: ACR75947.1.
RefSeqiWP_012743042.1. NC_012781.1.
YP_002938081.1. NC_012781.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi515619.EUBREC_2207.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACR75947; ACR75947; EUBREC_2207.
KEGGiere:EUBREC_2207.
PATRICi21872549. VBIEubRec107985_1921.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227222.
KOiK00858.
OMAiLLWGESP.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciEREC515619:GHMX-2198-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33656 / VPI 0990.

Entry informationi

Entry nameiNADK_EUBR3
AccessioniPrimary (citable) accession number: C4ZCY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: April 29, 2015
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.