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C4Z4D3 (C4Z4D3_EUBE2) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047
EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:EUBELI_00604 EMBL ACR71617.1
OrganismEubacterium eligens (strain ATCC 27750 / VPI C15-48) [Complete proteome] [HAMAP] EMBL ACR71617.1
Taxonomic identifier515620 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Eubacteriaceae › Eubacterium ›

Protein attributes

Sequence length382 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047 SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain184 – 375192ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding210 – 26152ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3291Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3421Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3421Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3441Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
C4Z4D3 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: A59303CBBE1F7778

FASTA38242,463
        10         20         30         40         50         60 
MDYINILQLS DNFTKNLQLT KNVNNYVDIV EKFRDKGEIM RIVVLAGGTS TERDVSLVTG 

        70         80         90        100        110        120 
YRVCESLRRN GHEANMLDIF LGVDDSEVKT FFTEKNDLGE LSDNLKKKTA DIPAEVKRRT 

       130        140        150        160        170        180 
EARESFFGRN VLELCKEADM VFMGLHGSNG EDGKVQATFD LLGIRYTGTD YLSSAISMSK 

       190        200        210        220        230        240 
ELAKKVLVPE GIPMPKGVTL HKGHKIEYVP FPCVVKPCCG GSSVGVSIAH NEKEFEDALD 

       250        260        270        280        290        300 
EAFSYEDTVL VEEFVDGREF SVAVLDGKAL PVIEIEPLEG FYDYKNKYKA GSTKETCPAN 

       310        320        330        340        350        360 
IPEDIASQMQ FWAEKCCQAA GVTTYARVDE LLDKNNNIFC LEINTLPGMT DTSLIPQEAA 

       370        380 
AVGISYDELT EKIIEISLAK YE

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References

[1]"Characterizing a model human gut microbiota composed of members of its two dominant bacterial phyla."
Mahowald M.A., Rey F.E., Seedorf H., Turnbaugh P.J., Fulton R.S., Wollam A., Shah N., Wang C., Magrini V., Wilson R.K., Cantarel B.L., Coutinho P.M., Henrissat B., Crock L.W., Russell A., Verberkmoes N.C., Hettich R.L., Gordon J.I.
Proc. Natl. Acad. Sci. U.S.A. 106:5859-5864(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27750 / VPI C15-48.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001104 Genomic DNA. Translation: ACR71617.1.
RefSeqYP_002930064.1. NC_012778.1.

3D structure databases

ProteinModelPortalC4Z4D3.
ModBaseSearch...

Protein-protein interaction databases

STRING515620.EUBELI_00604.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACR71617; ACR71617; EUBELI_00604.
GeneID7957478.
KEGGeel:EUBELI_00604.
PATRIC21864386. VBIEubEli113401_0558.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAVEDASCD.
ProtClustDBCLSK2505994.

Enzyme and pathway databases

BioCycEELI515620:GH1N-1397-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC4Z4D3_EUBE2
AccessionPrimary (citable) accession number: C4Z4D3
Entry history
Integrated into UniProtKB/TrEMBL: July 28, 2009
Last sequence update: July 28, 2009
Last modified: May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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