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C4Z4C1 (ASSY_EUBE2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:EUBELI_00592
OrganismEubacterium eligens (strain ATCC 27750 / VPI C15-48) [Complete proteome] [HAMAP]
Taxonomic identifier515620 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesEubacteriaceaeEubacterium

Protein attributes

Sequence length408 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00005

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 408408Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_1000201681

Regions

Nucleotide binding8 – 169ATP By similarity

Sites

Binding site351ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding site861Citrulline By similarity
Binding site911Citrulline By similarity
Binding site1161ATP; via amide nitrogen By similarity
Binding site1181Aspartate By similarity
Binding site1221Aspartate By similarity
Binding site1221Citrulline By similarity
Binding site1231Aspartate By similarity
Binding site1261Citrulline By similarity
Binding site1771Citrulline By similarity
Binding site1861Citrulline By similarity
Binding site2631Citrulline By similarity
Binding site2751Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
C4Z4C1 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: 991DDE21C7F2B43C

FASTA40846,072
        10         20         30         40         50         60 
MKEKVILAYS GGLDTTAIIP WLKETYNYDV VCVCADCGQE EELDGLEQRA LSCGAAKLYI 

        70         80         90        100        110        120 
EDITDEFCDN YIVPCVQAHA VYENKYLLGT SMARPLIAKR LVEIARKEGA VAICHGATGK 

       130        140        150        160        170        180 
GNDQIRFELT IKALAPDIKI IAPWRDSNWK LQSREDEIEY CRQHGIHLPF STDCSYSRDR 

       190        200        210        220        230        240 
NIWHISHEGL ELEDPANEPN YKHLLVLGCT PEEAPDEPEY VTMTFEKGVP KSVNGKAMKV 

       250        260        270        280        290        300 
SDIIRELNRL GAKHGIGIID IVENRVVGMK SRGVYETPGG TILYEAHQQL EELVLDRDTT 

       310        320        330        340        350        360 
TFKMDVGNKF AQVVYEGKWE TPLREALQAF VEKTQEYVTG EVKFRLYKGN IIKAGTTSPY 

       370        380        390        400 
SLYNESIASF KTGDMYDHHD ADGFINLFGL SLKVRAMKKL ENEKKNNK 

« Hide

References

[1]"Characterizing a model human gut microbiota composed of members of its two dominant bacterial phyla."
Mahowald M.A., Rey F.E., Seedorf H., Turnbaugh P.J., Fulton R.S., Wollam A., Shah N., Wang C., Magrini V., Wilson R.K., Cantarel B.L., Coutinho P.M., Henrissat B., Crock L.W., Russell A., Verberkmoes N.C., Hettich R.L., Gordon J.I.
Proc. Natl. Acad. Sci. U.S.A. 106:5859-5864(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27750 / VPI C15-48.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001104 Genomic DNA. Translation: ACR71605.1.
RefSeqYP_002930052.1. NC_012778.1.

3D structure databases

ProteinModelPortalC4Z4C1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING515620.EUBELI_00592.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACR71605; ACR71605; EUBELI_00592.
GeneID7958651.
KEGGeel:EUBELI_00592.
PATRIC21864362. VBIEubEli113401_0546.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230093.
KOK01940.
OMAAPPEEAY.
OrthoDBEOG6K9QCV.

Enzyme and pathway databases

BioCycEELI515620:GH1N-592-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_EUBE2
AccessionPrimary (citable) accession number: C4Z4C1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: May 14, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways