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Protein

Argininosuccinate synthase

Gene

argG

Organism
Eubacterium eligens (strain ATCC 27750 / VPI C15-48)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathway:iL-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei86 – 861CitrullineUniRule annotation
Binding sitei91 – 911CitrullineUniRule annotation
Binding sitei116 – 1161ATP; via amide nitrogenUniRule annotation
Binding sitei118 – 1181AspartateUniRule annotation
Binding sitei122 – 1221AspartateUniRule annotation
Binding sitei122 – 1221CitrullineUniRule annotation
Binding sitei123 – 1231AspartateUniRule annotation
Binding sitei126 – 1261CitrullineUniRule annotation
Binding sitei177 – 1771CitrullineUniRule annotation
Binding sitei186 – 1861CitrullineUniRule annotation
Binding sitei263 – 2631CitrullineUniRule annotation
Binding sitei275 – 2751CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 169ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEELI515620:GH1N-592-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:EUBELI_00592
OrganismiEubacterium eligens (strain ATCC 27750 / VPI C15-48)
Taxonomic identifieri515620 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesEubacteriaceaeEubacterium
ProteomesiUP000001476 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 408408Argininosuccinate synthasePRO_1000201681Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi515620.EUBELI_00592.

Structurei

3D structure databases

ProteinModelPortaliC4Z4C1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiIYNGYWW.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C4Z4C1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEKVILAYS GGLDTTAIIP WLKETYNYDV VCVCADCGQE EELDGLEQRA
60 70 80 90 100
LSCGAAKLYI EDITDEFCDN YIVPCVQAHA VYENKYLLGT SMARPLIAKR
110 120 130 140 150
LVEIARKEGA VAICHGATGK GNDQIRFELT IKALAPDIKI IAPWRDSNWK
160 170 180 190 200
LQSREDEIEY CRQHGIHLPF STDCSYSRDR NIWHISHEGL ELEDPANEPN
210 220 230 240 250
YKHLLVLGCT PEEAPDEPEY VTMTFEKGVP KSVNGKAMKV SDIIRELNRL
260 270 280 290 300
GAKHGIGIID IVENRVVGMK SRGVYETPGG TILYEAHQQL EELVLDRDTT
310 320 330 340 350
TFKMDVGNKF AQVVYEGKWE TPLREALQAF VEKTQEYVTG EVKFRLYKGN
360 370 380 390 400
IIKAGTTSPY SLYNESIASF KTGDMYDHHD ADGFINLFGL SLKVRAMKKL

ENEKKNNK
Length:408
Mass (Da):46,072
Last modified:July 28, 2009 - v1
Checksum:i991DDE21C7F2B43C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001104 Genomic DNA. Translation: ACR71605.1.
RefSeqiWP_012738841.1. NC_012778.1.

Genome annotation databases

EnsemblBacteriaiACR71605; ACR71605; EUBELI_00592.
KEGGieel:EUBELI_00592.
PATRICi21864362. VBIEubEli113401_0546.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001104 Genomic DNA. Translation: ACR71605.1.
RefSeqiWP_012738841.1. NC_012778.1.

3D structure databases

ProteinModelPortaliC4Z4C1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi515620.EUBELI_00592.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACR71605; ACR71605; EUBELI_00592.
KEGGieel:EUBELI_00592.
PATRICi21864362. VBIEubEli113401_0546.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiIYNGYWW.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciEELI515620:GH1N-592-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27750 / VPI C15-48.

Entry informationi

Entry nameiASSY_EUBE2
AccessioniPrimary (citable) accession number: C4Z4C1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: July 22, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.