C4Z478 (PUR5_EUBE2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	EUBELI_00549

Organism

Eubacterium eligens (strain ATCC 27750 / VPI C15-48) [Complete proteome] [HAMAP]

Taxonomic identifier

515620 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Eubacteriaceae › Eubacterium › Eubacterium eligens

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00741
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00741
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00741.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP-Rule MF_00741		PRO_1000212820

Sequences

	Sequence		Length	Mass (Da)
	C4Z478 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: 9C97457875213CF0	FASTA	341	36,215
	10 20 30 40 50 60 MDYKKSGVDI EAGYKSVELM KEAVKSTMRE EVLGGIGGFS GAFSLAKIKE MEEPVLLSGT 70 80 90 100 110 120 DGCGTKVKLA FIMDKHDTIG IDAVAMCVND VVCAGGEPLF FLDYIACGKN YPEKIATIVG 130 140 150 160 170 180 GVAEGCRQSE CALIGGETAE HPGLMPQDEY DLAGFAVGVV DKKDLITGEN IKPGDTLIGI 190 200 210 220 230 240 ASSGVHSNGF SLVRSVFTMT EESLNTYYDS LGKTLGEALL APTKIYVKTM KEIKKAGVKV 250 260 270 280 290 300 KGCSHITGGG FYENVPRMLP DGVKAVIKKD SYEVPPIFKM LAEDGNIEEH MMYNTFNMGI 310 320 330 340 GLVLAVDPAD VDTVMEAVKA AGETPYVIGS IEAGEKGVTL C

References

[1]

"Characterizing a model human gut microbiota composed of members of its two dominant bacterial phyla." Mahowald M.A., Rey F.E., Seedorf H., Turnbaugh P.J., Fulton R.S., Wollam A., Shah N., Wang C., Magrini V., Wilson R.K., Cantarel B.L., Coutinho P.M., Henrissat B., Crock L.W., Russell A., Verberkmoes N.C., Hettich R.L., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 106:5859-5864(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27750 / VPI C15-48.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001104 Genomic DNA. Translation: ACR71562.1.
RefSeq	YP_002930009.1. NC_012778.1.
3D structure databases
ProteinModelPortal	C4Z478.
ModBase	Search...
Protein-protein interaction databases
STRING	515620.EUBELI_00549.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACR71562; ACR71562; EUBELI_00549.
GeneID	7958608.
KEGG	eel:EUBELI_00549.
PATRIC	21864282. VBIEubEli113401_0506.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0150.
HOGENOM	HOG000229091.
KO	K01933.
OMA	IDMIAMN.
ProtClustDB	CLSK2506417.
Enzyme and pathway databases
BioCyc	EELI515620:GH1N-549-MONOMER.
UniPathway	UPA00074; UER00129.
Family and domain databases
Gene3D	3.90.650.10. 1 hit.
HAMAP	MF_00741_B. AIRS_B.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. purM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_EUBE2

Accession

Primary (citable) accession number: C4Z478

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 28, 2009
Last modified:	May 29, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program