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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

DUT1

Organism
Candida albicans (strain WO-1) (Yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.

Cofactori

Mg2+By similarity

Pathwayi: dUMP biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Deoxyuridine 5'-triphosphate nucleotidohydrolase (DUT1)
This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei103Substrate; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei148SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
dUTP pyrophosphatase
Gene namesi
Name:DUT1
ORF Names:CAWG_01243
OrganismiCandida albicans (strain WO-1) (Yeast)
Taxonomic identifieri294748 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
Proteomesi
  • UP000001429 Componenti: Chromosome 1, Supercontig 1.1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004130361 – 159Deoxyuridine 5'-triphosphate nucleotidohydrolaseAdd BLAST159

Interactioni

Subunit structurei

Homotrimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliC4YFC7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni78 – 80Substrate bindingBy similarity3
Regioni92 – 95Substrate bindingBy similarity4
Regioni153 – 154Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the dUTPase family.Curated

Phylogenomic databases

HOGENOMiHOG000028966.
OMAiKICYPEL.
OrthoDBiEOG092C5PPT.

Family and domain databases

CDDicd07557. trimeric_dUTPase. 1 hit.
Gene3Di2.70.40.10. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
IPR033704. dUTPase_trimeric.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

C4YFC7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSEDQSLKK QKLESTQSLK VYLRSPKGKV PTKGSALAAG YDLYSAEAAT
60 70 80 90 100
IPAHGQGLVS TDISIIVPIG TYGRVAPRSG LAVKHGISTG AGVIDADYRG
110 120 130 140 150
EVKVVLFNHS EKDFEIKEGD RIAQLVLEQI VNADIKEISL EELDNTERGE

GGFGSTGKN
Length:159
Mass (Da):16,945
Last modified:July 28, 2009 - v1
Checksum:i982C016F1D31013A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77925 Genomic DNA. Translation: CAA54897.1.
CH672346 Genomic DNA. Translation: EEQ43013.1.

Genome annotation databases

EnsemblFungiiEEQ43013; EEQ43013; CAWG_01243.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77925 Genomic DNA. Translation: CAA54897.1.
CH672346 Genomic DNA. Translation: EEQ43013.1.

3D structure databases

ProteinModelPortaliC4YFC7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEEQ43013; EEQ43013; CAWG_01243.

Phylogenomic databases

HOGENOMiHOG000028966.
OMAiKICYPEL.
OrthoDBiEOG092C5PPT.

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666.

Family and domain databases

CDDicd07557. trimeric_dUTPase. 1 hit.
Gene3Di2.70.40.10. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
IPR033704. dUTPase_trimeric.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDUT_CANAW
AccessioniPrimary (citable) accession number: C4YFC7
Secondary accession number(s): P43058, Q5A912
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: July 28, 2009
Last modified: October 5, 2016
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.