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C4YA37

- LIPA_CLAL4

UniProt

C4YA37 - LIPA_CLAL4

Protein

Lipoyl synthase, mitochondrial

Gene

CLUG_04975

Organism
Clavispora lusitaniae (strain ATCC 42720) (Yeast) (Candida lusitaniae)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 32 (01 Oct 2014)
      Sequence version 1 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi120 – 1201Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi125 – 1251Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi131 – 1311Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi150 – 1501Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi154 – 1541Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi157 – 1571Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    ORF Names:CLUG_04975
    OrganismiClavispora lusitaniae (strain ATCC 42720) (Yeast) (Candida lusitaniae)
    Taxonomic identifieri306902 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesMetschnikowiaceaeClavispora
    ProteomesiUP000007703: Unassembled WGS sequence

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4444MitochondrionUniRule annotationAdd
    BLAST
    Chaini45 – 391347Lipoyl synthase, mitochondrialPRO_0000398262Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliC4YA37.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    KOiK03644.
    OrthoDBiEOG79KPR7.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    C4YA37-1 [UniParc]FASTAAdd to Basket

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    MVHPHLSRTK RTFFSHSSQM ISRHIRKTNS LAFVRALSAS ETAVTPRRKR    50
    TVFTDELNKG PSFEDFVSGK AADVFVDPLE AARKDPEAKL PKWLKVPIPK 100
    GRSFHSVKKD VRELKLATVC EEAKCPNISE CWGGKKSEAT ATIMLLGDTC 150
    TRGCRFCSVK TSRTPAKPDP MEPENTAEAI SRWGLGYVVL TTVDRDDLAD 200
    GGAAHLAETV RLIKQKAPQI LVEVLGGDFR GDLTSCDTLA VSGLDVYAHN 250
    LETVEALTPH VRDRRATYRQ SLSILERAKQ AKPSLITKTS LMLGFGETDE 300
    QIMQTLRDLR GIGCDVVTFG QYMRPTKRHM KVVEYVRPEK FDYWKEVALE 350
    MGFLYVASGP LVRSSYKAGE AFIENVLRKR KHNVGDSPRL A 391
    Length:391
    Mass (Da):43,682
    Last modified:July 28, 2009 - v1
    Checksum:iEE362CDEE68AFA88
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH408081 Genomic DNA. Translation: EEQ40847.1.
    RefSeqiXP_002614960.1. XM_002614914.1.

    Genome annotation databases

    GeneIDi8495745.
    KEGGiclu:CLUG_04975.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH408081 Genomic DNA. Translation: EEQ40847.1 .
    RefSeqi XP_002614960.1. XM_002614914.1.

    3D structure databases

    ProteinModelPortali C4YA37.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 8495745.
    KEGGi clu:CLUG_04975.

    Phylogenomic databases

    KOi K03644.
    OrthoDBi EOG79KPR7.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 42720.

    Entry informationi

    Entry nameiLIPA_CLAL4
    AccessioniPrimary (citable) accession number: C4YA37
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 32 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3