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C4YA37

- LIPA_CLAL4

UniProt

C4YA37 - LIPA_CLAL4

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Protein

Lipoyl synthase, mitochondrial

Gene

CLUG_04975

Organism
Clavispora lusitaniae (strain ATCC 42720) (Yeast) (Candida lusitaniae)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi120 – 1201Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi125 – 1251Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi131 – 1311Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi150 – 1501Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi154 – 1541Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi157 – 1571Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
ORF Names:CLUG_04975
OrganismiClavispora lusitaniae (strain ATCC 42720) (Yeast) (Candida lusitaniae)
Taxonomic identifieri306902 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesMetschnikowiaceaeClavispora
ProteomesiUP000007703: Unassembled WGS sequence

Subcellular locationi

Mitochondrion UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4444MitochondrionUniRule annotationAdd
BLAST
Chaini45 – 391347Lipoyl synthase, mitochondrialPRO_0000398262Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliC4YA37.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

InParanoidiC4YA37.
KOiK03644.
OrthoDBiEOG79KPR7.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C4YA37-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVHPHLSRTK RTFFSHSSQM ISRHIRKTNS LAFVRALSAS ETAVTPRRKR
60 70 80 90 100
TVFTDELNKG PSFEDFVSGK AADVFVDPLE AARKDPEAKL PKWLKVPIPK
110 120 130 140 150
GRSFHSVKKD VRELKLATVC EEAKCPNISE CWGGKKSEAT ATIMLLGDTC
160 170 180 190 200
TRGCRFCSVK TSRTPAKPDP MEPENTAEAI SRWGLGYVVL TTVDRDDLAD
210 220 230 240 250
GGAAHLAETV RLIKQKAPQI LVEVLGGDFR GDLTSCDTLA VSGLDVYAHN
260 270 280 290 300
LETVEALTPH VRDRRATYRQ SLSILERAKQ AKPSLITKTS LMLGFGETDE
310 320 330 340 350
QIMQTLRDLR GIGCDVVTFG QYMRPTKRHM KVVEYVRPEK FDYWKEVALE
360 370 380 390
MGFLYVASGP LVRSSYKAGE AFIENVLRKR KHNVGDSPRL A
Length:391
Mass (Da):43,682
Last modified:July 28, 2009 - v1
Checksum:iEE362CDEE68AFA88
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH408081 Genomic DNA. Translation: EEQ40847.1.
RefSeqiXP_002614960.1. XM_002614914.1.

Genome annotation databases

GeneIDi8495745.
KEGGiclu:CLUG_04975.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH408081 Genomic DNA. Translation: EEQ40847.1 .
RefSeqi XP_002614960.1. XM_002614914.1.

3D structure databases

ProteinModelPortali C4YA37.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 8495745.
KEGGi clu:CLUG_04975.

Phylogenomic databases

InParanoidi C4YA37.
KOi K03644.
OrthoDBi EOG79KPR7.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 42720.

Entry informationi

Entry nameiLIPA_CLAL4
AccessioniPrimary (citable) accession number: C4YA37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 28, 2009
Last modified: October 29, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3