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Protein

Probable glutamate--tRNA ligase, cytoplasmic

Gene

NCER_102160

Organism
Nosema ceranae (strain BRL01) (Microsporidian parasite)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei140 – 1401ATPBy similarity
Binding sitei166 – 1661GlutamateBy similarity
Binding sitei321 – 3211GlutamateBy similarity
Binding sitei324 – 3241ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi359 – 3635ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamate--tRNA ligase, cytoplasmic (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
ORF Names:NCER_102160
OrganismiNosema ceranae (strain BRL01) (Microsporidian parasite)
Taxonomic identifieri578460 [NCBI]
Taxonomic lineageiEukaryotaFungiMicrosporidiaNosematidaeNosema
ProteomesiUP000009082 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiMicrosporidiaDB:NCER_102160.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 614614Probable glutamate--tRNA ligase, cytoplasmicPRO_0000388393Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliC4VBI7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni130 – 1323Glutamate bindingBy similarity
Regioni303 – 3075Glutamate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi135 – 14410"HIGH" regionBy similarity
Motifi359 – 3635"KMSKS" regionBy similarity

Sequence similaritiesi

Phylogenomic databases

InParanoidiC4VBI7.
KOiK01885.
OrthoDBiEOG7MKWFN.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C4VBI7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVENAINFR EICSLLDISE SYKCTINAFL DNVEKCTDKH YNDALNLLDT
60 70 80 90 100
FFSNEVKSNS LVNDLIFCII NSNFDFLKVF KSNKLRLENL QIVYESAFNE
110 120 130 140 150
NKPFLKEFSA KDKINKEVKK NLYSTPAVTR FAPEPSGCLH IGHLKALLVN
160 170 180 190 200
YNLAEKSNGT LLLRFDDTNP VKNYEKYEKE ILRDLDTLGI TGLKISHSSD
210 220 230 240 250
YFELLVDEAV SLINKNLAYV DNTDQETMRI ERFEGIESKM RNINNSESLK
260 270 280 290 300
IFKELLQGRA PGYCLRAKID MSNPNKSMRD PVIYRASDKM HGRCKLYKAF
310 320 330 340 350
PTYDFVCPIV DSIEGVTVVC RANEYKDRNE QYKWFLENLE LENKPEFNDF
360 370 380 390 400
SKLNLEDTVL SKRKIDKLIS DSLVTGWDDP RLATIQGIKR LGMHMTALKD
410 420 430 440 450
YINLQGASNK TNVISWDKIW AMNKKVIDPL SPRFMAVEKI NCVRVFITNF
460 470 480 490 500
EGLKYTKNIP LNKKNTSLGS KDVLFSDTLL FSQEDGFVLK ENEEFTLMNW
510 520 530 540 550
GNAIVEKKVV ENSIVTELYI KLHLEGDYKS TTNKISWVSE SGAVTATGIE
560 570 580 590 600
YGKLLVNEEF NINSKIDKQY YVESSITNLS TDMKHVQFER IGFFYCDSPC
610
VFHLVPFTKQ KRTY
Length:614
Mass (Da):70,755
Last modified:July 28, 2009 - v1
Checksum:iDD63B17BF1DEA37C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ACOL01000581 Genomic DNA. Translation: EEQ81415.1.
RefSeqiXP_002995086.1. XM_002995040.1.

Genome annotation databases

GeneIDi9423469.
KEGGince:NCER_102160.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ACOL01000581 Genomic DNA. Translation: EEQ81415.1.
RefSeqiXP_002995086.1. XM_002995040.1.

3D structure databases

ProteinModelPortaliC4VBI7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi9423469.
KEGGince:NCER_102160.

Organism-specific databases

EuPathDBiMicrosporidiaDB:NCER_102160.

Phylogenomic databases

InParanoidiC4VBI7.
KOiK01885.
OrthoDBiEOG7MKWFN.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genomic analyses of the microsporidian Nosema ceranae, an emergent pathogen of honey bees."
    Cornman R.S., Chen Y.P., Schatz M.C., Street C., Zhao Y., Desany B., Egholm M., Hutchison S., Pettis J.S., Lipkin W.I., Evans J.D.
    PLoS Pathog. 5:E1000466-E1000466(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BRL01.

Entry informationi

Entry nameiSYEC_NOSCE
AccessioniPrimary (citable) accession number: C4VBI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: July 28, 2009
Last modified: January 7, 2015
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.