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C4VBI7 (SYEC_NOSCE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable glutamate--tRNA ligase, cytoplasmic

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
ORF Names:NCER_102160
OrganismNosema ceranae (strain BRL01) (Microsporidian parasite) [Complete proteome]
Taxonomic identifier578460 [NCBI]
Taxonomic lineageEukaryotaFungiMicrosporidiaNosematidaeNosema

Protein attributes

Sequence length614 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA aminoacylation for protein translation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 614614Probable glutamate--tRNA ligase, cytoplasmic
PRO_0000388393

Regions

Nucleotide binding359 – 3635ATP By similarity
Region130 – 1323Glutamate binding By similarity
Region303 – 3075Glutamate binding By similarity
Motif135 – 14410"HIGH" region By similarity
Motif359 – 3635"KMSKS" region By similarity

Sites

Binding site1401ATP By similarity
Binding site1661Glutamate By similarity
Binding site3211Glutamate By similarity
Binding site3241ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
C4VBI7 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: DD63B17BF1DEA37C

FASTA61470,755
        10         20         30         40         50         60 
MAVENAINFR EICSLLDISE SYKCTINAFL DNVEKCTDKH YNDALNLLDT FFSNEVKSNS 

        70         80         90        100        110        120 
LVNDLIFCII NSNFDFLKVF KSNKLRLENL QIVYESAFNE NKPFLKEFSA KDKINKEVKK 

       130        140        150        160        170        180 
NLYSTPAVTR FAPEPSGCLH IGHLKALLVN YNLAEKSNGT LLLRFDDTNP VKNYEKYEKE 

       190        200        210        220        230        240 
ILRDLDTLGI TGLKISHSSD YFELLVDEAV SLINKNLAYV DNTDQETMRI ERFEGIESKM 

       250        260        270        280        290        300 
RNINNSESLK IFKELLQGRA PGYCLRAKID MSNPNKSMRD PVIYRASDKM HGRCKLYKAF 

       310        320        330        340        350        360 
PTYDFVCPIV DSIEGVTVVC RANEYKDRNE QYKWFLENLE LENKPEFNDF SKLNLEDTVL 

       370        380        390        400        410        420 
SKRKIDKLIS DSLVTGWDDP RLATIQGIKR LGMHMTALKD YINLQGASNK TNVISWDKIW 

       430        440        450        460        470        480 
AMNKKVIDPL SPRFMAVEKI NCVRVFITNF EGLKYTKNIP LNKKNTSLGS KDVLFSDTLL 

       490        500        510        520        530        540 
FSQEDGFVLK ENEEFTLMNW GNAIVEKKVV ENSIVTELYI KLHLEGDYKS TTNKISWVSE 

       550        560        570        580        590        600 
SGAVTATGIE YGKLLVNEEF NINSKIDKQY YVESSITNLS TDMKHVQFER IGFFYCDSPC 

       610 
VFHLVPFTKQ KRTY 

« Hide

References

[1]"Genomic analyses of the microsporidian Nosema ceranae, an emergent pathogen of honey bees."
Cornman R.S., Chen Y.P., Schatz M.C., Street C., Zhao Y., Desany B., Egholm M., Hutchison S., Pettis J.S., Lipkin W.I., Evans J.D.
PLoS Pathog. 5:E1000466-E1000466(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BRL01.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
ACOL01000581 Genomic DNA. Translation: EEQ81415.1.
RefSeqXP_002995086.1. XM_002995040.1.

3D structure databases

ProteinModelPortalC4VBI7.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID9423469.
KEGGnce:NCER_102160.

Organism-specific databases

EuPathDBMicrosporidiaDB:NCER_102160.

Phylogenomic databases

KOK01885.
OrthoDBEOG7MKWFN.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYEC_NOSCE
AccessionPrimary (citable) accession number: C4VBI7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: July 28, 2009
Last modified: November 13, 2013
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries