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Protein

Probable isoleucine--tRNA ligase, cytoplasmic

Gene

NCER_100885

Organism
Nosema ceranae (strain BRL01) (Microsporidian parasite)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei618 – 6181ATPBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. isoleucine-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable isoleucine--tRNA ligase, cytoplasmic (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
ORF Names:NCER_100885
OrganismiNosema ceranae (strain BRL01) (Microsporidian parasite)
Taxonomic identifieri578460 [NCBI]
Taxonomic lineageiEukaryotaFungiMicrosporidiaNosematidaeNosema
ProteomesiUP000009082 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiMicrosporidiaDB:NCER_100885.

Subcellular locationi

  1. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10461046Probable isoleucine--tRNA ligase, cytoplasmicPRO_0000388380Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliC4V8Q1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi46 – 5611"HIGH" regionBy similarityAdd
BLAST
Motifi615 – 6195"KMSKS" regionBy similarity

Sequence similaritiesi

Phylogenomic databases

InParanoidiC4V8Q1.
KOiK01870.
OrthoDBiEOG7FNCGS.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

C4V8Q1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFDSNLSIN DIETKILKFW KDTKCFKKSN ELSKNKKKYT FYDGPPFATG
60 70 80 90 100
LPHFGHILAG TVKDVITRYQ YQHNKRVDRR FGWDCHGLPV EFEIDKNLGI
110 120 130 140 150
SVKQEILEMG IDKYNAECKS IVMKHSSEWK DTVEKMGRWV DFDNSYKTMD
160 170 180 190 200
LSFMTSVWYV FSQLYKKGYV YRGYRVMPFS TGCMTTLSSS EAKSNYKMVN
210 220 230 240 250
DLSVVVEFPL KSKLFDKKVS ILAWTTTPWT LPSNCGLVVN KDFDYQIFEI
260 270 280 290 300
DHKFYCMLPN RIQDFNKKEV ILHELFKGEL LIGLEYEQPF NYFEEYRRCG
310 320 330 340 350
FFKIIGGSFV SSTDGTGIVH AAPAFGEDDY NCFVENNLIK QNDLVPCPVD
360 370 380 390 400
ENGKFTAEVF DYKGIYVKDA DKLIIKHLKE KIFCVKQISH NYPFCWRSEK
410 420 430 440 450
PLIYRLVSSW FIKVSDSVDK LIKNNEIINW VPKDIKHKKF GKWLSNAKDW
460 470 480 490 500
AFSRSRFWGT PIPLWVSDDY SEILCVESAE ELEKLSGKKI TDLHMEFIDD
510 520 530 540 550
IILTKNGKTL RRIPEVFDCW FESGCMPYAQ HSWPFRKVDN LNCEEIRHHF
560 570 580 590 600
HDSDNTLVYE NKILENFPAD FIGEGIDQTR GWFYTLHVIS TLLFDKPAFK
610 620 630 640 650
NVIVNGIVLA ENGKKMSKKD KNYPDPNIVM KTFGADSLRM YLISSPVVEA
660 670 680 690 700
DNLLFKEDGV KDVSKLLIIP WMNVLKFYTT SLQKRNDCEK LELDNWINYT
710 720 730 740 750
FNEFLSSVSN YMNNYQLSKV CGLAYKFLDN LSNWYLRIHR EEIRSGNTKI
760 770 780 790 800
LFNILKKFSV IMSPFAPFFS EYSFQCLLSS QQNEKSNSIN SVHFQMYPEA
810 820 830 840 850
QDSGDNSFEN AKDIIDAIRY LRDKHTISLK TPLKEVKIIS DELFMKDAAK
860 870 880 890 900
FSNTIIRECH VFNLIFVKES EVPELKIEYK AKPCFEYLKK DLKTMNDKIK
910 920 930 940 950
IINKLTQSEI RDLAFLDLSE EKYNVKRENI LIEKKATFPT GLCFASNKFV
960 970 980 990 1000
ILVDNTIDDN VLEKKLAREF NSFIQKLRKS CGLKMNDTVN VEVDSEKIKQ
1010 1020 1030 1040
ITKKFYPINF STEGQIVSPI DSDAQIDVFS YEGQELKVIL RLNKFL
Length:1,046
Mass (Da):122,145
Last modified:July 28, 2009 - v1
Checksum:iB2FCC7669CD0CEF7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ACOL01000062 Genomic DNA. Translation: EEQ82400.1.
RefSeqiXP_002996071.1. XM_002996025.1.

Genome annotation databases

GeneIDi9422100.
KEGGince:NCER_100885.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ACOL01000062 Genomic DNA. Translation: EEQ82400.1.
RefSeqiXP_002996071.1. XM_002996025.1.

3D structure databases

ProteinModelPortaliC4V8Q1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi9422100.
KEGGince:NCER_100885.

Organism-specific databases

EuPathDBiMicrosporidiaDB:NCER_100885.

Phylogenomic databases

InParanoidiC4V8Q1.
KOiK01870.
OrthoDBiEOG7FNCGS.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genomic analyses of the microsporidian Nosema ceranae, an emergent pathogen of honey bees."
    Cornman R.S., Chen Y.P., Schatz M.C., Street C., Zhao Y., Desany B., Egholm M., Hutchison S., Pettis J.S., Lipkin W.I., Evans J.D.
    PLoS Pathog. 5:E1000466-E1000466(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BRL01.

Entry informationi

Entry nameiSYIC_NOSCE
AccessioniPrimary (citable) accession number: C4V8Q1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: July 28, 2009
Last modified: January 7, 2015
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.