C4V8Q1 (SYIC_NOSCE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable isoleucine--tRNA ligase, cytoplasmic
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

ORF Names:

NCER_100885

Organism

Nosema ceranae (strain BRL01) (Microsporidian parasite) [Complete proteome]

Taxonomic identifier

578460 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Microsporidia › Apansporoblastina › Nosematidae › Nosema › Nosema ceranae

Protein attributes

Sequence length	1046 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1046	1046	Probable isoleucine--tRNA ligase, cytoplasmic		PRO_0000388380
Regions
	Motif	46 – 56	11	"HIGH" region By similarity
	Motif	615 – 619	5	"KMSKS" region By similarity
Sites
	Binding site	618	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	C4V8Q1 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: B2FCC7669CD0CEF7	FASTA	1,046	122,145
	10 20 30 40 50 60 MKFDSNLSIN DIETKILKFW KDTKCFKKSN ELSKNKKKYT FYDGPPFATG LPHFGHILAG 70 80 90 100 110 120 TVKDVITRYQ YQHNKRVDRR FGWDCHGLPV EFEIDKNLGI SVKQEILEMG IDKYNAECKS 130 140 150 160 170 180 IVMKHSSEWK DTVEKMGRWV DFDNSYKTMD LSFMTSVWYV FSQLYKKGYV YRGYRVMPFS 190 200 210 220 230 240 TGCMTTLSSS EAKSNYKMVN DLSVVVEFPL KSKLFDKKVS ILAWTTTPWT LPSNCGLVVN 250 260 270 280 290 300 KDFDYQIFEI DHKFYCMLPN RIQDFNKKEV ILHELFKGEL LIGLEYEQPF NYFEEYRRCG 310 320 330 340 350 360 FFKIIGGSFV SSTDGTGIVH AAPAFGEDDY NCFVENNLIK QNDLVPCPVD ENGKFTAEVF 370 380 390 400 410 420 DYKGIYVKDA DKLIIKHLKE KIFCVKQISH NYPFCWRSEK PLIYRLVSSW FIKVSDSVDK 430 440 450 460 470 480 LIKNNEIINW VPKDIKHKKF GKWLSNAKDW AFSRSRFWGT PIPLWVSDDY SEILCVESAE 490 500 510 520 530 540 ELEKLSGKKI TDLHMEFIDD IILTKNGKTL RRIPEVFDCW FESGCMPYAQ HSWPFRKVDN 550 560 570 580 590 600 LNCEEIRHHF HDSDNTLVYE NKILENFPAD FIGEGIDQTR GWFYTLHVIS TLLFDKPAFK 610 620 630 640 650 660 NVIVNGIVLA ENGKKMSKKD KNYPDPNIVM KTFGADSLRM YLISSPVVEA DNLLFKEDGV 670 680 690 700 710 720 KDVSKLLIIP WMNVLKFYTT SLQKRNDCEK LELDNWINYT FNEFLSSVSN YMNNYQLSKV 730 740 750 760 770 780 CGLAYKFLDN LSNWYLRIHR EEIRSGNTKI LFNILKKFSV IMSPFAPFFS EYSFQCLLSS 790 800 810 820 830 840 QQNEKSNSIN SVHFQMYPEA QDSGDNSFEN AKDIIDAIRY LRDKHTISLK TPLKEVKIIS 850 860 870 880 890 900 DELFMKDAAK FSNTIIRECH VFNLIFVKES EVPELKIEYK AKPCFEYLKK DLKTMNDKIK 910 920 930 940 950 960 IINKLTQSEI RDLAFLDLSE EKYNVKRENI LIEKKATFPT GLCFASNKFV ILVDNTIDDN 970 980 990 1000 1010 1020 VLEKKLAREF NSFIQKLRKS CGLKMNDTVN VEVDSEKIKQ ITKKFYPINF STEGQIVSPI 1030 1040 DSDAQIDVFS YEGQELKVIL RLNKFL

References

[1]

"Genomic analyses of the microsporidian Nosema ceranae, an emergent pathogen of honey bees." Cornman R.S., Chen Y.P., Schatz M.C., Street C., Zhao Y., Desany B., Egholm M., Hutchison S., Pettis J.S., Lipkin W.I., Evans J.D. PLoS Pathog. 5:E1000466-E1000466(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BRL01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	ACOL01000062 Genomic DNA. Translation: EEQ82400.1.
RefSeq	XP_002996071.1. XM_002996025.1.
3D structure databases
ProteinModelPortal	C4V8Q1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	9422100.
KEGG	nce:NCER_100885.
Organism-specific databases
EuPathDB	MicrosporidiaDB:NCER_100885.
Phylogenomic databases
KO	K01870.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
InterPro	IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYIC_NOSCE

Accession

Primary (citable) accession number: C4V8Q1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	July 28, 2009
Last modified:	March 6, 2013
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program