Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

C4V8Q1 (SYIC_NOSCE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable isoleucine--tRNA ligase, cytoplasmic

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
ORF Names:NCER_100885
OrganismNosema ceranae (strain BRL01) (Microsporidian parasite) [Complete proteome]
Taxonomic identifier578460 [NCBI]
Taxonomic lineageEukaryotaFungiMicrosporidiaNosematidaeNosema

Protein attributes

Sequence length1046 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10461046Probable isoleucine--tRNA ligase, cytoplasmic
PRO_0000388380

Regions

Motif46 – 5611"HIGH" region By similarity
Motif615 – 6195"KMSKS" region By similarity

Sites

Binding site6181ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
C4V8Q1 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: B2FCC7669CD0CEF7

FASTA1,046122,145
        10         20         30         40         50         60 
MKFDSNLSIN DIETKILKFW KDTKCFKKSN ELSKNKKKYT FYDGPPFATG LPHFGHILAG 

        70         80         90        100        110        120 
TVKDVITRYQ YQHNKRVDRR FGWDCHGLPV EFEIDKNLGI SVKQEILEMG IDKYNAECKS 

       130        140        150        160        170        180 
IVMKHSSEWK DTVEKMGRWV DFDNSYKTMD LSFMTSVWYV FSQLYKKGYV YRGYRVMPFS 

       190        200        210        220        230        240 
TGCMTTLSSS EAKSNYKMVN DLSVVVEFPL KSKLFDKKVS ILAWTTTPWT LPSNCGLVVN 

       250        260        270        280        290        300 
KDFDYQIFEI DHKFYCMLPN RIQDFNKKEV ILHELFKGEL LIGLEYEQPF NYFEEYRRCG 

       310        320        330        340        350        360 
FFKIIGGSFV SSTDGTGIVH AAPAFGEDDY NCFVENNLIK QNDLVPCPVD ENGKFTAEVF 

       370        380        390        400        410        420 
DYKGIYVKDA DKLIIKHLKE KIFCVKQISH NYPFCWRSEK PLIYRLVSSW FIKVSDSVDK 

       430        440        450        460        470        480 
LIKNNEIINW VPKDIKHKKF GKWLSNAKDW AFSRSRFWGT PIPLWVSDDY SEILCVESAE 

       490        500        510        520        530        540 
ELEKLSGKKI TDLHMEFIDD IILTKNGKTL RRIPEVFDCW FESGCMPYAQ HSWPFRKVDN 

       550        560        570        580        590        600 
LNCEEIRHHF HDSDNTLVYE NKILENFPAD FIGEGIDQTR GWFYTLHVIS TLLFDKPAFK 

       610        620        630        640        650        660 
NVIVNGIVLA ENGKKMSKKD KNYPDPNIVM KTFGADSLRM YLISSPVVEA DNLLFKEDGV 

       670        680        690        700        710        720 
KDVSKLLIIP WMNVLKFYTT SLQKRNDCEK LELDNWINYT FNEFLSSVSN YMNNYQLSKV 

       730        740        750        760        770        780 
CGLAYKFLDN LSNWYLRIHR EEIRSGNTKI LFNILKKFSV IMSPFAPFFS EYSFQCLLSS 

       790        800        810        820        830        840 
QQNEKSNSIN SVHFQMYPEA QDSGDNSFEN AKDIIDAIRY LRDKHTISLK TPLKEVKIIS 

       850        860        870        880        890        900 
DELFMKDAAK FSNTIIRECH VFNLIFVKES EVPELKIEYK AKPCFEYLKK DLKTMNDKIK 

       910        920        930        940        950        960 
IINKLTQSEI RDLAFLDLSE EKYNVKRENI LIEKKATFPT GLCFASNKFV ILVDNTIDDN 

       970        980        990       1000       1010       1020 
VLEKKLAREF NSFIQKLRKS CGLKMNDTVN VEVDSEKIKQ ITKKFYPINF STEGQIVSPI 

      1030       1040 
DSDAQIDVFS YEGQELKVIL RLNKFL 

« Hide

References

[1]"Genomic analyses of the microsporidian Nosema ceranae, an emergent pathogen of honey bees."
Cornman R.S., Chen Y.P., Schatz M.C., Street C., Zhao Y., Desany B., Egholm M., Hutchison S., Pettis J.S., Lipkin W.I., Evans J.D.
PLoS Pathog. 5:E1000466-E1000466(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BRL01.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
ACOL01000062 Genomic DNA. Translation: EEQ82400.1.
RefSeqXP_002996071.1. XM_002996025.1.

3D structure databases

ProteinModelPortalC4V8Q1.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID9422100.
KEGGnce:NCER_100885.

Organism-specific databases

EuPathDBMicrosporidiaDB:NCER_100885.

Phylogenomic databases

KOK01870.
OrthoDBEOG7FNCGS.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYIC_NOSCE
AccessionPrimary (citable) accession number: C4V8Q1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: July 28, 2009
Last modified: April 16, 2014
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries