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C4V8L1 (SYLC_NOSCE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable leucine--tRNA ligase, cytoplasmic

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
ORF Names:NCER_100842
OrganismNosema ceranae (strain BRL01) (Microsporidian parasite) [Complete proteome]
Taxonomic identifier578460 [NCBI]
Taxonomic lineageEukaryotaFungiMicrosporidiaNosematidaeNosema

Protein attributes

Sequence length884 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA aminoacylation for protein translation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 884884Probable leucine--tRNA ligase, cytoplasmic
PRO_0000388415

Regions

Motif40 – 5011"HIGH" region By similarity
Motif566 – 5705"KMSKS" region By similarity

Sites

Binding site5691ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
C4V8L1 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: 909334958DC35622

FASTA884102,520
        10         20         30         40         50         60 
MNCNEHESTK LNYLNSLEKS RDVKTDISDK PKYFITFPYP YMNGKLHLGH LYSISKADFM 

        70         80         90        100        110        120 
SYYKELQGFN VLFPLAFHCT GMPIAALAKK LGEELEGKKT DISTKEIIQN LGFNDVMPFT 

       130        140        150        160        170        180 
DPVHWIKTFP KLCISSLKTF GANIDWRRSF VTTDINKYYD SFVRWQFFNL KELGYLSFGK 

       190        200        210        220        230        240 
RHSIYCPLDK QMCLDHDRRK GENIKPVRQI MFKFHLHDKI LLVRQNSIGK PYKLVCGKSL 

       250        260        270        280        290        300 
EVVSFKYNNT SFLAEKIIFD NLKYQINNVK YKESLVLSMD LKDGLTYDGT NIEVEVIEKN 

       310        320        330        340        350        360 
ILVVKTDTKS DVELYEKEIK ALNEIEETLF TLSTSSEGIV ESVAKLSVEN TKDLLVQTKH 

       370        380        390        400        410        420 
FISVYIPEKE VISRSGGICV VSLLDQWYID YSNEKWKSKV KKCLDNLECG PDTRSMLYAG 

       430        440        450        460        470        480 
IDWVNKWGFS RSFGLGTKIP WDTQYLIDSL SDSTIYMAFY TVKHLLFEDL EGKLEIFPSN 

       490        500        510        520        530        540 
KLSNDVWDYI FAGKDLVPEL YGYLDLLEKC RNNFQYFYPV DLRVSGKDLI KNHLLFFMFN 

       550        560        570        580        590        600 
HVALFDEKYW PKRIFTNGHL MLNKEKMSKS TGNFLSVDDA LLKYGKSATR MCLAVCGDTN 

       610        620        630        640        650        660 
DDANFVEDNA NLFILKIYTF VKEIQKLNEL VRKKSENETI ASYSNADVLL LETVSVNVTE 

       670        680        690        700        710        720 
ALNAYNSMKY SDVVKFSFFE MVNLINLYNN INGTNILLKN LVYKSATQLL YPIMPDLCRT 

       730        740        750        760        770        780 
LIEKYFDSEF NLPEPKLKTN KMISAFEYIS ELIKKIAASK QAKNNSCVKI AVGKNYSEWK 

       790        800        810        820        830        840 
KEIFELIDKI DCPRNNEIKK NKVFIASLLD SVKPILQKSK INITKGNNFV MDYLVNPDKF 

       850        860        870        880 
VMKFNEYEII NEFKFYIENM SNKKAILEEH CDAEPLNPII TFSK 

« Hide

References

[1]"Genomic analyses of the microsporidian Nosema ceranae, an emergent pathogen of honey bees."
Cornman R.S., Chen Y.P., Schatz M.C., Street C., Zhao Y., Desany B., Egholm M., Hutchison S., Pettis J.S., Lipkin W.I., Evans J.D.
PLoS Pathog. 5:E1000466-E1000466(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BRL01.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
ACOL01000057 Genomic DNA. Translation: EEQ82449.1.
RefSeqXP_002996120.1. XM_002996074.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEC4V8L1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID9424018.
KEGGnce:NCER_100842.

Organism-specific databases

EuPathDBMicrosporidiaDB:NCER_100842.

Phylogenomic databases

KOK01869.
OrthoDBEOG7TTQH2.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamPF00133. tRNA-synt_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYLC_NOSCE
AccessionPrimary (citable) accession number: C4V8L1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: July 28, 2009
Last modified: April 16, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries