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Protein

Probable leucine--tRNA ligase, cytoplasmic

Gene

NCER_100842

Organism
Nosema ceranae (strain BRL01) (Microsporidian parasite)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei569 – 5691ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. leucine-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. tRNA aminoacylation for protein translation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable leucine--tRNA ligase, cytoplasmic (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
ORF Names:NCER_100842
OrganismiNosema ceranae (strain BRL01) (Microsporidian parasite)
Taxonomic identifieri578460 [NCBI]
Taxonomic lineageiEukaryotaFungiMicrosporidiaNosematidaeNosema
ProteomesiUP000009082 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiMicrosporidiaDB:NCER_100842.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 884884Probable leucine--tRNA ligase, cytoplasmicPRO_0000388415Add
BLAST

Proteomic databases

PRIDEiC4V8L1.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5011"HIGH" regionBy similarityAdd
BLAST
Motifi566 – 5705"KMSKS" regionBy similarity

Sequence similaritiesi

Phylogenomic databases

InParanoidiC4V8L1.
KOiK01869.
OrthoDBiEOG7TTQH2.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamiPF00133. tRNA-synt_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C4V8L1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNCNEHESTK LNYLNSLEKS RDVKTDISDK PKYFITFPYP YMNGKLHLGH
60 70 80 90 100
LYSISKADFM SYYKELQGFN VLFPLAFHCT GMPIAALAKK LGEELEGKKT
110 120 130 140 150
DISTKEIIQN LGFNDVMPFT DPVHWIKTFP KLCISSLKTF GANIDWRRSF
160 170 180 190 200
VTTDINKYYD SFVRWQFFNL KELGYLSFGK RHSIYCPLDK QMCLDHDRRK
210 220 230 240 250
GENIKPVRQI MFKFHLHDKI LLVRQNSIGK PYKLVCGKSL EVVSFKYNNT
260 270 280 290 300
SFLAEKIIFD NLKYQINNVK YKESLVLSMD LKDGLTYDGT NIEVEVIEKN
310 320 330 340 350
ILVVKTDTKS DVELYEKEIK ALNEIEETLF TLSTSSEGIV ESVAKLSVEN
360 370 380 390 400
TKDLLVQTKH FISVYIPEKE VISRSGGICV VSLLDQWYID YSNEKWKSKV
410 420 430 440 450
KKCLDNLECG PDTRSMLYAG IDWVNKWGFS RSFGLGTKIP WDTQYLIDSL
460 470 480 490 500
SDSTIYMAFY TVKHLLFEDL EGKLEIFPSN KLSNDVWDYI FAGKDLVPEL
510 520 530 540 550
YGYLDLLEKC RNNFQYFYPV DLRVSGKDLI KNHLLFFMFN HVALFDEKYW
560 570 580 590 600
PKRIFTNGHL MLNKEKMSKS TGNFLSVDDA LLKYGKSATR MCLAVCGDTN
610 620 630 640 650
DDANFVEDNA NLFILKIYTF VKEIQKLNEL VRKKSENETI ASYSNADVLL
660 670 680 690 700
LETVSVNVTE ALNAYNSMKY SDVVKFSFFE MVNLINLYNN INGTNILLKN
710 720 730 740 750
LVYKSATQLL YPIMPDLCRT LIEKYFDSEF NLPEPKLKTN KMISAFEYIS
760 770 780 790 800
ELIKKIAASK QAKNNSCVKI AVGKNYSEWK KEIFELIDKI DCPRNNEIKK
810 820 830 840 850
NKVFIASLLD SVKPILQKSK INITKGNNFV MDYLVNPDKF VMKFNEYEII
860 870 880
NEFKFYIENM SNKKAILEEH CDAEPLNPII TFSK
Length:884
Mass (Da):102,520
Last modified:July 28, 2009 - v1
Checksum:i909334958DC35622
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ACOL01000057 Genomic DNA. Translation: EEQ82449.1.
RefSeqiXP_002996120.1. XM_002996074.1.

Genome annotation databases

GeneIDi9424018.
KEGGince:NCER_100842.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ACOL01000057 Genomic DNA. Translation: EEQ82449.1.
RefSeqiXP_002996120.1. XM_002996074.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiC4V8L1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi9424018.
KEGGince:NCER_100842.

Organism-specific databases

EuPathDBiMicrosporidiaDB:NCER_100842.

Phylogenomic databases

InParanoidiC4V8L1.
KOiK01869.
OrthoDBiEOG7TTQH2.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamiPF00133. tRNA-synt_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genomic analyses of the microsporidian Nosema ceranae, an emergent pathogen of honey bees."
    Cornman R.S., Chen Y.P., Schatz M.C., Street C., Zhao Y., Desany B., Egholm M., Hutchison S., Pettis J.S., Lipkin W.I., Evans J.D.
    PLoS Pathog. 5:E1000466-E1000466(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BRL01.

Entry informationi

Entry nameiSYLC_NOSCE
AccessioniPrimary (citable) accession number: C4V8L1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: July 28, 2009
Last modified: January 7, 2015
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.