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C4R2P3

- MAP2_PICPG

UniProt

C4R2P3 - MAP2_PICPG

Protein

Methionine aminopeptidase 2

Gene

MAP2

Organism
Komagataella pastoris (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 38 (01 Oct 2014)
      Sequence version 1 (07 Jul 2009)
      Previous versions | rss
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    Functioni

    Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

    Catalytic activityi

    Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

    Cofactori

    Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei198 – 1981SubstrateUniRule annotation
    Metal bindingi218 – 2181Divalent metal cation 1UniRule annotation
    Metal bindingi229 – 2291Divalent metal cation 1UniRule annotation
    Metal bindingi229 – 2291Divalent metal cation 2; catalyticUniRule annotation
    Metal bindingi298 – 2981Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation
    Binding sitei306 – 3061SubstrateUniRule annotation
    Metal bindingi331 – 3311Divalent metal cation 2; catalyticUniRule annotation
    Metal bindingi429 – 4291Divalent metal cation 1UniRule annotation
    Metal bindingi429 – 4291Divalent metal cation 2; catalyticUniRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-HAMAP
    2. metalloaminopeptidase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. protein initiator methionine removal Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Protease

    Keywords - Ligandi

    Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methionine aminopeptidase 2UniRule annotation (EC:3.4.11.18UniRule annotation)
    Short name:
    MAP 2UniRule annotation
    Short name:
    MetAP 2UniRule annotation
    Alternative name(s):
    Peptidase MUniRule annotation
    Gene namesi
    Name:MAP2UniRule annotation
    Ordered Locus Names:PAS_chr2-2_0159
    OrganismiKomagataella pastoris (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)
    Taxonomic identifieri644223 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKomagataella
    ProteomesiUP000000314: Chromosome 2

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 448448Methionine aminopeptidase 2PRO_0000407664Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi644223.PAS_chr2-2_0159.

    Structurei

    3D structure databases

    ProteinModelPortaliC4R2P3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0024.
    HOGENOMiHOG000226278.
    KOiK01265.
    OrthoDBiEOG7BGHW3.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    3.90.230.10. 2 hits.
    HAMAPiMF_03175. MetAP_2_euk.
    InterProiIPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002468. Pept_M24A_MAP2.
    IPR018349. Pept_M24A_MAP2_BS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PANTHERiPTHR10804:SF9. PTHR10804:SF9. 1 hit.
    PfamiPF00557. Peptidase_M24. 1 hit.
    [Graphical view]
    PRINTSiPR00599. MAPEPTIDASE.
    SUPFAMiSSF55920. SSF55920. 2 hits.
    TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
    PROSITEiPS01202. MAP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C4R2P3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSSVDKVSQ KVADVKLGSS KSTKNNKSKG KGKSNKNQVV EDDDEDDFEK    50
    ALELAMQLDA QKLAQKKADD VPLVEEEEKK VEEKIEQQYD PISTFYPDGN 100
    YPQGEVVDYK DDNLYRTTDE EKRALDREKN NKWNEFRKGA EIHRRVRKLA 150
    KDEIKPGMSM IEIAELIENA VRGYSGEDGL KGGMGFPCGL SLNHCAAHYS 200
    PNANDKLVLN YEDVMKVDFG VHVNGHIIDS AFTLTFDDKY DDLLKAVKDA 250
    TNTGIREAGI DVRLTDIGEA IQEVMESYEV TLDGETYQVK PIKNLCGHNI 300
    GQYRIHGGKS VPIVKNFDNT KMEEGETFAI ETFGSTGRGH VIGQGECSHY 350
    AKNPDAPANA ISSIRVNRAK QLLKTIDENF GTLPFCRRYI DRLGEEKYLL 400
    ALNQLVKSGV VSDYPPLVDV KGSYTAQYEH TILLRPNVKE VVSRGEDY 448
    Length:448
    Mass (Da):50,204
    Last modified:July 7, 2009 - v1
    Checksum:i6BCE8381B94A08F1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN392320 Genomic DNA. Translation: CAY69767.1.
    RefSeqiXP_002492047.1. XM_002492002.1.

    Genome annotation databases

    EnsemblFungiiCAY69767; CAY69767; PAS_chr2-2_0159.
    GeneIDi8198269.
    KEGGippa:PAS_chr2-2_0159.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN392320 Genomic DNA. Translation: CAY69767.1 .
    RefSeqi XP_002492047.1. XM_002492002.1.

    3D structure databases

    ProteinModelPortali C4R2P3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 644223.PAS_chr2-2_0159.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CAY69767 ; CAY69767 ; PAS_chr2-2_0159 .
    GeneIDi 8198269.
    KEGGi ppa:PAS_chr2-2_0159.

    Phylogenomic databases

    eggNOGi COG0024.
    HOGENOMi HOG000226278.
    KOi K01265.
    OrthoDBi EOG7BGHW3.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    3.90.230.10. 2 hits.
    HAMAPi MF_03175. MetAP_2_euk.
    InterProi IPR001714. Pept_M24_MAP.
    IPR000994. Pept_M24_structural-domain.
    IPR002468. Pept_M24A_MAP2.
    IPR018349. Pept_M24A_MAP2_BS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PANTHERi PTHR10804:SF9. PTHR10804:SF9. 1 hit.
    Pfami PF00557. Peptidase_M24. 1 hit.
    [Graphical view ]
    PRINTSi PR00599. MAPEPTIDASE.
    SUPFAMi SSF55920. SSF55920. 2 hits.
    TIGRFAMsi TIGR00501. met_pdase_II. 1 hit.
    PROSITEi PS01202. MAP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GS115 / ATCC 20864.

    Entry informationi

    Entry nameiMAP2_PICPG
    AccessioniPrimary (citable) accession number: C4R2P3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: July 7, 2009
    Last modified: October 1, 2014
    This is version 38 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3