Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2

Gene

PAS_chr2-2_0346

Organism
Komagataella pastoris (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.UniRule annotation

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.UniRule annotation

Pathway: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

TransferaseUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2UniRule annotation (EC:2.4.99.18UniRule annotation)
Short name:
Oligosaccharyl transferase subunit OST2UniRule annotation
Gene namesi
Ordered Locus Names:PAS_chr2-2_0346Imported
OrganismiKomagataella pastoris (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)Imported
Taxonomic identifieri644223 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesPhaffomycetaceaeKomagataella
ProteomesiUP000000314 Componenti: Chromosome 2

Subcellular locationi

  • Endoplasmic reticulum membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei49 – 7224HelicalUniRule annotationAdd
BLAST
Transmembranei78 – 9619HelicalUniRule annotationAdd
BLAST
Transmembranei116 – 13419HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulumUniRule annotation, Membrane

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex.UniRule annotation

Protein-protein interaction databases

STRINGi644223.XP_002491840.1.

Family & Domainsi

Sequence similaritiesi

Belongs to the DAD/OST2 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helixUniRule annotation

Phylogenomic databases

eggNOGiNOG246737.
HOGENOMiHOG000211854.
InParanoidiC4R236.
KOiK12668.
OrthoDBiEOG757D9B.

Family and domain databases

InterProiIPR003038. DAD/Ost2.
[Graphical view]
PANTHERiPTHR10705. PTHR10705. 1 hit.
PfamiPF02109. DAD. 1 hit.
[Graphical view]
PIRSFiPIRSF005588. DAD. 1 hit.

Sequencei

Sequence statusi: Complete.

C4R236-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKHPTQQS ATVNPLSLTR SLPHELSEAI STSLASYQKS ISGNRKLRLI
60 70 80 90 100
DYFLTFLVFL GVLQFAFCLL VGTFPFNAFL GGFCSTVAQF VLTISLRLQT
110 120 130
TGTKEIFQNI SSQRAFAEYI FASLLLHFIV YHFIN
Length:135
Mass (Da):15,162
Last modified:July 7, 2009 - v1
Checksum:i39911E75D344E046
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN392320 Genomic DNA. Translation: CAY69560.1.
RefSeqiXP_002491840.1. XM_002491795.1.

Genome annotation databases

EnsemblFungiiCAY69560; CAY69560; PAS_chr2-2_0346.
GeneIDi8198246.
KEGGippa:PAS_chr2-2_0346.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN392320 Genomic DNA. Translation: CAY69560.1.
RefSeqiXP_002491840.1. XM_002491795.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi644223.XP_002491840.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAY69560; CAY69560; PAS_chr2-2_0346.
GeneIDi8198246.
KEGGippa:PAS_chr2-2_0346.

Phylogenomic databases

eggNOGiNOG246737.
HOGENOMiHOG000211854.
InParanoidiC4R236.
KOiK12668.
OrthoDBiEOG757D9B.

Enzyme and pathway databases

UniPathwayiUPA00378.

Family and domain databases

InterProiIPR003038. DAD/Ost2.
[Graphical view]
PANTHERiPTHR10705. PTHR10705. 1 hit.
PfamiPF02109. DAD. 1 hit.
[Graphical view]
PIRSFiPIRSF005588. DAD. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GS115 / ATCC 20864Imported.

Entry informationi

Entry nameiC4R236_PICPG
AccessioniPrimary (citable) accession number: C4R236
Entry historyi
Integrated into UniProtKB/TrEMBL: July 7, 2009
Last sequence update: July 7, 2009
Last modified: June 24, 2015
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.