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C4R142 (C4R142_PICPG) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isocitrate dehydrogenase [NADP] PIRNR PIRNR000108
EC=1.1.1.42 PIRNR PIRNR000108
Gene names
Ordered Locus Names:PAS_chr2-1_0580
OrganismKomagataella pastoris (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris) [Complete proteome]
Taxonomic identifier644223 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Komagataella ›

Protein attributes

Sequence length432 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. PIRNR PIRNR000108

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity. PIRNR PIRNR000108 PIRSR PIRSR000108-3

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family. PIRNR PIRNR000108 RuleBase RU004443

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding97 – 993NADP By similarity PIRSR PIRSR000108-4
Nucleotide binding331 – 3366NADP By similarity PIRSR PIRSR000108-4
Region116 – 1227Substrate binding By similarity PIRSR PIRSR000108-2

Sites

Metal binding2731Magnesium or manganese By similarity PIRSR PIRSR000108-3
Metal binding2961Magnesium or manganese By similarity PIRSR PIRSR000108-3
Binding site991Substrate By similarity PIRSR PIRSR000108-2
Binding site1041NADP By similarity PIRSR PIRSR000108-4
Binding site1311Substrate By similarity PIRSR PIRSR000108-2
Binding site1541Substrate By similarity PIRSR PIRSR000108-2
Binding site2811NADP By similarity PIRSR PIRSR000108-4
Binding site3491NADP; via amide nitrogen and carbonyl oxygen By similarity PIRSR PIRSR000108-4
Site1611Critical for catalysis By similarity PIRSR PIRSR000108-1
Site2331Critical for catalysis By similarity PIRSR PIRSR000108-1

Sequences

Sequence LengthMass (Da)Tools
C4R142 [UniParc].

Last modified July 7, 2009. Version 1.
Checksum: 17B43CA9069AFC76

FASTA43248,755
        10         20         30         40         50         60 
MGKVTVFHLF FFFLGISYCK MAHAKISVKT PLVEMDGDEM TRIIWKLIKD ELILPFLDID 

        70         80         90        100        110        120 
LKYYDLGIEY RDQTDDQVTI DAAEAIKKYG VGVKCATITP DEARVEEFGL KKMWLSPNGT 

       130        140        150        160        170        180 
IRNILGGTVF REPIVIDNIP RIIPQWEKPI IIGRHAYGDQ YRATDLLIPK AGELKLVFTP 

       190        200        210        220        230        240 
KDGSDPVETK VFDYPSAGVA LTMYNLDDSI RDFALSSFKL ALEKKVNLFS TTKNTILKKY 

       250        260        270        280        290        300 
DGRFKDIFDE TYETQFKESF EKAGIWYEHR LIDDMVAQML KSKGGYIIAM KNYDGDVQSD 

       310        320        330        340        350        360 
IVAQGFGSLG LMTSVLTTPD GTAFESEAAH GTVTRHYRQH QQGKETSTNS IASIFAWTRG 

       370        380        390        400        410        420 
LIQRGLLDNT LPVVEFGQLL ENATINTVKL DGIMTKDLAL ARGETDRSSY VNTEEFIKAV 

       430 
AKRLTSEFEA KF

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References

[1]"Genome sequence of the recombinant protein production host Pichia pastoris."
De Schutter K., Lin Y.-C., Tiels P., Van Hecke A., Glinka S., Weber-Lehmann J., Rouze P., Van de Peer Y., Callewaert N.
Nat. Biotechnol. 27:561-566(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GS115 / ATCC 20864.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FN392320 Genomic DNA. Translation: CAY69216.1.
RefSeqXP_002491496.1. XM_002491451.1.

3D structure databases

ProteinModelPortalC4R142.
ModBaseSearch...

Protein-protein interaction databases

STRING644223.PAS_chr2-1_0580.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCAY69216; CAY69216; PAS_chr2-1_0580.
GeneID8198933.
KEGGppa:PAS_chr2-1_0580.

Phylogenomic databases

eggNOGCOG0538.
HOGENOMHOG000019858.
KOK00031.
OrthoDBEOG47M56G.

Family and domain databases

Gene3D3.40.718.10. 1 hit.
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERPTHR11822. PTHR11822. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsTIGR00127. nadp_idh_euk. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC4R142_PICPG
AccessionPrimary (citable) accession number: C4R142
Entry history
Integrated into UniProtKB/TrEMBL: July 7, 2009
Last sequence update: July 7, 2009
Last modified: May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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