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Protein

Isocitrate dehydrogenase [NADP]

Gene

PAS_chr2-1_0580

Organism
Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 Mg2+ or Mn2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99SubstrateUniRule annotation1
Binding sitei104NADPUniRule annotation1
Binding sitei131SubstrateUniRule annotation1
Binding sitei154SubstrateUniRule annotation1
Sitei161Critical for catalysisUniRule annotation1
Sitei233Critical for catalysisUniRule annotation1
Metal bindingi273Magnesium or manganeseUniRule annotation1
Binding sitei281NADPUniRule annotation1
Metal bindingi296Magnesium or manganeseUniRule annotation1
Binding sitei349NADP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi97 – 99NADPUniRule annotation3
Nucleotide bindingi331 – 336NADPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

Tricarboxylic acid cycleUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
Gene namesi
Ordered Locus Names:PAS_chr2-1_0580Imported
OrganismiKomagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)Imported
Taxonomic identifieri644223 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesPhaffomycetaceaeKomagataella
Proteomesi
  • UP000000314 Componenti: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_500294215425 – 432Isocitrate dehydrogenase [NADP]Sequence analysisAdd BLAST408

Interactioni

Protein-protein interaction databases

STRINGi644223.XP_002491496.1.

Structurei

3D structure databases

ProteinModelPortaliC4R142.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 420Iso_dhInterPro annotationAdd BLAST390

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni116 – 122Substrate bindingUniRule annotation7

Sequence similaritiesi

Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

Keywords - Domaini

SignalSequence analysis

Phylogenomic databases

eggNOGiKOG1526. Eukaryota.
COG0538. LUCA.
HOGENOMiHOG000019858.
InParanoidiC4R142.
KOiK00031.
OMAiISEASCG.
OrthoDBiEOG092C2D51.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C4R142-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKVTVFHLF FFFLGISYCK MAHAKISVKT PLVEMDGDEM TRIIWKLIKD
60 70 80 90 100
ELILPFLDID LKYYDLGIEY RDQTDDQVTI DAAEAIKKYG VGVKCATITP
110 120 130 140 150
DEARVEEFGL KKMWLSPNGT IRNILGGTVF REPIVIDNIP RIIPQWEKPI
160 170 180 190 200
IIGRHAYGDQ YRATDLLIPK AGELKLVFTP KDGSDPVETK VFDYPSAGVA
210 220 230 240 250
LTMYNLDDSI RDFALSSFKL ALEKKVNLFS TTKNTILKKY DGRFKDIFDE
260 270 280 290 300
TYETQFKESF EKAGIWYEHR LIDDMVAQML KSKGGYIIAM KNYDGDVQSD
310 320 330 340 350
IVAQGFGSLG LMTSVLTTPD GTAFESEAAH GTVTRHYRQH QQGKETSTNS
360 370 380 390 400
IASIFAWTRG LIQRGLLDNT LPVVEFGQLL ENATINTVKL DGIMTKDLAL
410 420 430
ARGETDRSSY VNTEEFIKAV AKRLTSEFEA KF
Length:432
Mass (Da):48,755
Last modified:July 7, 2009 - v1
Checksum:i17B43CA9069AFC76
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN392320 Genomic DNA. Translation: CAY69216.1.
RefSeqiXP_002491496.1. XM_002491451.1.

Genome annotation databases

EnsemblFungiiCAY69216; CAY69216; PAS_chr2-1_0580.
GeneIDi8198933.
KEGGippa:PAS_chr2-1_0580.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN392320 Genomic DNA. Translation: CAY69216.1.
RefSeqiXP_002491496.1. XM_002491451.1.

3D structure databases

ProteinModelPortaliC4R142.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi644223.XP_002491496.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAY69216; CAY69216; PAS_chr2-1_0580.
GeneIDi8198933.
KEGGippa:PAS_chr2-1_0580.

Phylogenomic databases

eggNOGiKOG1526. Eukaryota.
COG0538. LUCA.
HOGENOMiHOG000019858.
InParanoidiC4R142.
KOiK00031.
OMAiISEASCG.
OrthoDBiEOG092C2D51.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC4R142_KOMPG
AccessioniPrimary (citable) accession number: C4R142
Entry historyi
Integrated into UniProtKB/TrEMBL: July 7, 2009
Last sequence update: July 7, 2009
Last modified: November 2, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.