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Protein

Lipoyl synthase, mitochondrial

Gene

PAS_chr1-4_0425

Organism
Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Miscellaneous

This protein may be expected to contain an N-terminal transit peptide but none has been predicted.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N6-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (PAS_chr1-1_0243)
  2. Lipoyl synthase, mitochondrial (PAS_chr1-4_0425)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi122Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi127Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi133Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi152Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi156Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi159Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
Ordered Locus Names:PAS_chr1-4_0425
OrganismiKomagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)
Taxonomic identifieri644223 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesPhaffomycetaceaeKomagataella
Proteomesi
  • UP000000314 Componenti: Chromosome 1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003982821 – 417Lipoyl synthase, mitochondrialAdd BLAST417

Interactioni

Protein-protein interaction databases

STRINGi644223.XP_002490556.1.

Structurei

3D structure databases

ProteinModelPortaliC4QYF2.
SMRiC4QYF2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2672. Eukaryota.
COG0320. LUCA.
HOGENOMiHOG000235998.
InParanoidiC4QYF2.
KOiK03644.
OMAiPYCDIDF.
OrthoDBiEOG092C13O2.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR031691. LIAS_N.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
PfamiView protein in Pfam
PF16881. LIAS_N. 1 hit.
PF04055. Radical_SAM. 1 hit.
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SFLDiSFLDG01058. lipoyl_synthase_like. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
SMARTiView protein in SMART
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

C4QYF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSTRVVRLP KVISGGNKQL ARGLASLSDE KSLNEANPTD LAGLKRKAKR
60 70 80 90 100
RPTKLADELK TGPSFADFVT GKAKDMLVDP LELARNDPNA RLPSWLKTQI
110 120 130 140 150
PKGKSFHHLK SDLKELKLST VCEEAKCPNI GECWGGKKSE ATATIMLMGD
160 170 180 190 200
TCTRGCRFCS VKTNRNPAPP DPNEPENTAE AISRWGLGYV VLTTVDRDDL
210 220 230 240 250
PDGGAHHLAS TVTKIKEKAP QILVECLSGD FRGNLEMAKV LASSPLDVFA
260 270 280 290 300
HNLETVEDLT PHIRDRRATY RQSLSVLRAA KEANPRLVTK TSLMLGFGET
310 320 330 340 350
DDQILQTLHD LRNISCDVVT FGQYMRPTKR HMKVVEYVTP SKFEYWRDKA
360 370 380 390 400
LEMGFLYCAS GPLVRSSYKA GEAFIENVIK KRRTNVGAIE EQQHDKENNN
410
LLLSKEDEKT TQEKANF
Length:417
Mass (Da):46,464
Last modified:July 7, 2009 - v1
Checksum:i5AAFED84116F9660
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN392319 Genomic DNA. Translation: CAY68275.1.
RefSeqiXP_002490556.1. XM_002490511.1.

Genome annotation databases

EnsemblFungiiCAY68275; CAY68275; PAS_chr1-4_0425.
GeneIDi8197013.
KEGGippa:PAS_chr1-4_0425.

Similar proteinsi

Entry informationi

Entry nameiLIPA_KOMPG
AccessioniPrimary (citable) accession number: C4QYF2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 7, 2009
Last modified: October 25, 2017
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families