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C4QYF2

- LIPA_PICPG

UniProt

C4QYF2 - LIPA_PICPG

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Protein
Lipoyl synthase, mitochondrial
Gene
PAS_chr1-4_0425
Organism
Komagataella pastoris (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi122 – 1221Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi127 – 1271Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi133 – 1331Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi152 – 1521Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi156 – 1561Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi159 – 1591Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
Ordered Locus Names:PAS_chr1-4_0425
OrganismiKomagataella pastoris (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)
Taxonomic identifieri644223 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKomagataella
ProteomesiUP000000314: Chromosome 1

Subcellular locationi

Mitochondrion By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 417Lipoyl synthase, mitochondrialUniRule annotationPRO_0000398282
Transit peptidei1 – ?Mitochondrion Reviewed prediction

Interactioni

Protein-protein interaction databases

STRINGi644223.PAS_chr1-4_0425.

Structurei

3D structure databases

ProteinModelPortaliC4QYF2.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OrthoDBiEOG79KPR7.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C4QYF2-1 [UniParc]FASTAAdd to Basket

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MLSTRVVRLP KVISGGNKQL ARGLASLSDE KSLNEANPTD LAGLKRKAKR    50
RPTKLADELK TGPSFADFVT GKAKDMLVDP LELARNDPNA RLPSWLKTQI 100
PKGKSFHHLK SDLKELKLST VCEEAKCPNI GECWGGKKSE ATATIMLMGD 150
TCTRGCRFCS VKTNRNPAPP DPNEPENTAE AISRWGLGYV VLTTVDRDDL 200
PDGGAHHLAS TVTKIKEKAP QILVECLSGD FRGNLEMAKV LASSPLDVFA 250
HNLETVEDLT PHIRDRRATY RQSLSVLRAA KEANPRLVTK TSLMLGFGET 300
DDQILQTLHD LRNISCDVVT FGQYMRPTKR HMKVVEYVTP SKFEYWRDKA 350
LEMGFLYCAS GPLVRSSYKA GEAFIENVIK KRRTNVGAIE EQQHDKENNN 400
LLLSKEDEKT TQEKANF 417
Length:417
Mass (Da):46,464
Last modified:July 7, 2009 - v1
Checksum:i5AAFED84116F9660
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FN392319 Genomic DNA. Translation: CAY68275.1.
RefSeqiXP_002490556.1. XM_002490511.1.

Genome annotation databases

EnsemblFungiiCAY68275; CAY68275; PAS_chr1-4_0425.
GeneIDi8197013.
KEGGippa:PAS_chr1-4_0425.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FN392319 Genomic DNA. Translation: CAY68275.1 .
RefSeqi XP_002490556.1. XM_002490511.1.

3D structure databases

ProteinModelPortali C4QYF2.
ModBasei Search...

Protein-protein interaction databases

STRINGi 644223.PAS_chr1-4_0425.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CAY68275 ; CAY68275 ; PAS_chr1-4_0425 .
GeneIDi 8197013.
KEGGi ppa:PAS_chr1-4_0425.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OrthoDBi EOG79KPR7.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GS115 / ATCC 20864.

Entry informationi

Entry nameiLIPA_PICPG
AccessioniPrimary (citable) accession number: C4QYF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 7, 2009
Last modified: June 11, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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