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C4PRB0 (C4PRB0_CHLTJ) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Serine hydroxymethyltransferase HAMAP-Rule MF_00051
Short name=SHMT HAMAP-Rule MF_00051
Short name=Serine methylase HAMAP-Rule MF_00051
EC=2.1.2.1 HAMAP-Rule MF_00051
Gene names
Name:glyA HAMAP-Rule MF_00051 EMBL CAX10887.1
Ordered Locus Names:JALI_4331 EMBL CAX10887.1
OrganismChlamydia trachomatis serovar B (strain Jali20/OT) [Complete proteome] [HAMAP] EMBL CAX10887.1
Taxonomic identifier580049 [NCBI]
Taxonomic lineageBacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia ›

Protein attributes

Sequence length497 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism By similarity. HAMAP-Rule MF_00051

Interconversion of serine and glycine By similarity. RuleBase RU000585

Catalytic activity

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP-Rule MF_00051 RuleBase RU000585

Cofactor

Pyridoxal phosphate By similarity. HAMAP-Rule MF_00051 RuleBase RU000585

Pathway

Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1. HAMAP-Rule MF_00051

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_00051 RuleBase RU000585

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00051

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00051.

Sequence similarities

Belongs to the SHMT family. HAMAP-Rule MF_00051 RuleBase RU004104

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region180 – 1823Substrate binding By similarity HAMAP-Rule MF_00051

Sites

Binding site591Pyridoxal phosphate By similarity HAMAP-Rule MF_00051
Binding site791Pyridoxal phosphate By similarity HAMAP-Rule MF_00051
Binding site811Substrate By similarity HAMAP-Rule MF_00051
Binding site881Substrate By similarity HAMAP-Rule MF_00051
Binding site891Pyridoxal phosphate By similarity HAMAP-Rule MF_00051
Binding site1761Substrate; via carbonyl oxygen By similarity HAMAP-Rule MF_00051
Binding site2321Pyridoxal phosphate By similarity HAMAP-Rule MF_00051
Binding site2601Pyridoxal phosphate By similarity HAMAP-Rule MF_00051
Binding site2881Pyridoxal phosphate By similarity HAMAP-Rule MF_00051
Binding site2951Pyridoxal phosphate By similarity HAMAP-Rule MF_00051
Binding site3191Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity HAMAP-Rule MF_00051
Binding site4191Pyridoxal phosphate By similarity HAMAP-Rule MF_00051

Amino acid modifications

Modified residue2891N6-(pyridoxal phosphate)lysine By similarity HAMAP-Rule MF_00051

Sequences

Sequence LengthMass (Da)Tools
C4PRB0 [UniParc].

Last modified July 7, 2009. Version 1.
Checksum: 73663EF9F07AA11F

FASTA49754,245
        10         20         30         40         50         60 
MASLLDRYLR NISDKSQQNL ASVAYSASLD HLLHAFPSIG QSIVQELKSQ RSRLKMIASE 

        70         80         90        100        110        120 
NFSSLSVQLA MGNLLTDKYC EGSPFKRFYS CCENVDAIEW ECAETAKELF GAESAFVQPH 

       130        140        150        160        170        180 
SGADANLLAI MSIITQKIQS PAVQRLGYKT INDLPEQEYE ALKAEMAQHK CLGPSLNSGG 

       190        200        210        220        230        240 
HLTHGTVRMN IMSKLMHCLP YEVNLDTELF DYDEIAKIAK EHKPTVLIAG YSSYSRRLNF 

       250        260        270        280        290        300 
ATLKQIAEDC GAVLWVDMAH FAGLVAGGVF VGEENPMPYA DIVTTTTHKT LRGPRGGLVL 

       310        320        330        340        350        360 
AKKEYANTLN KACPLMMGGP LPHVIAAKAI ALKEAMTINF RKYAHKVVEN ARTLAEVFQR 

       370        380        390        400        410        420 
NGLRLLTGGT DNHMLIIDLT SLGVPGRIAE DMLTSVGIAV NRNTIPSDAS GQWKTSGIRL 

       430        440        450        460        470        480 
GTPALTTLGM GSAEMEEVAN IIVKVLRNIT VRSNAESGSS KSEGELSEGI AQEARQRVAD 

       490 
LLGRFPLYPE IDLETLV

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References

[1]"Co-evolution of genomes and plasmids within Chlamydia trachomatis and the emergence in Sweden of a new variant strain."
Seth-Smith H.M., Harris S.R., Persson K., Marsh P., Barron A., Bignell A., Bjartling C., Clark L., Cutcliffe L.T., Lambden P.R., Lennard N., Lockey S.J., Quail M.A., Salim O., Skilton R.J., Wang Y., Holland M.J., Parkhill J., Thomson N.R., Clarke I.N.
BMC Genomics 10:239-239(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Jali20/OT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FM872308 Genomic DNA. Translation: CAX10887.1.
RefSeqYP_002888053.1. NC_012686.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING580049.JALI_4331.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAX10887; CAX10887; JALI_4331.
GeneID7882273.
KEGGctj:JALI_4331.
PATRIC20378430. VBIChlTra100303_0488.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0112.
HOGENOMHOG000239404.
KOK00600.
OMAVDLRESH.
ProtClustDBPRK13580.

Enzyme and pathway databases

BioCycCTRA580049:GH1T-545-MONOMER.
UniPathwayUPA00193.
UPA00288; UER01023.

Family and domain databases

Gene3D3.40.640.10. 2 hits.
3.90.1150.10. 1 hit.
HAMAPMF_00051. SHMT.
InterProIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
PANTHERPTHR11680. PTHR11680. 1 hit.
PfamPF00464. SHMT. 2 hits.
[Graphical view]
PIRSFPIRSF000412. SHMT. 1 hit.
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITEPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC4PRB0_CHLTJ
AccessionPrimary (citable) accession number: C4PRB0
Entry history
Integrated into UniProtKB/TrEMBL: July 7, 2009
Last sequence update: July 7, 2009
Last modified: May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info