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Protein

Serine hydroxymethyltransferase

Gene

glyA

Organism
Chlamydia trachomatis serovar B (strain Jali20/OT)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • pyridoxal 5'-phosphateUniRule annotation
  • Note: Pyridoxal phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei59 – 591Pyridoxal phosphateUniRule annotation
Binding sitei79 – 791Pyridoxal phosphateUniRule annotation
Binding sitei81 – 811SubstrateUniRule annotation
Binding sitei88 – 881SubstrateUniRule annotation
Binding sitei89 – 891Pyridoxal phosphateUniRule annotation
Binding sitei176 – 1761Substrate; via carbonyl oxygenUniRule annotation
Binding sitei232 – 2321Pyridoxal phosphateUniRule annotation
Binding sitei260 – 2601Pyridoxal phosphateUniRule annotation
Binding sitei288 – 2881Pyridoxal phosphateUniRule annotation
Binding sitei295 – 2951Pyridoxal phosphateUniRule annotation
Binding sitei319 – 3191Pyridoxal phosphate; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei419 – 4191Pyridoxal phosphateUniRule annotation

GO - Molecular functioni

  1. glycine hydroxymethyltransferase activity Source: UniProtKB-HAMAP
  2. methyltransferase activity Source: UniProtKB-KW
  3. pyridoxal phosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. glycine biosynthetic process from serine Source: UniProtKB-HAMAP
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

MethyltransferaseImported, Transferase

Keywords - Biological processi

Amino-acid biosynthesisUniRule annotation, One-carbon metabolismUniRule annotation

Keywords - Ligandi

Pyridoxal phosphateUniRule annotation

Enzyme and pathway databases

BioCyciCTRA580049:GH1T-458-MONOMER.
UniPathwayiUPA00193.
UPA00288; UER01023.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine hydroxymethyltransferaseUniRule annotation (EC:2.1.2.1UniRule annotation)
Short name:
SHMTUniRule annotation
Short name:
Serine methylaseUniRule annotation
Gene namesi
Name:glyAUniRule annotationImported
Ordered Locus Names:JALI_4331Imported
OrganismiChlamydia trachomatis serovar B (strain Jali20/OT)Imported
Taxonomic identifieri580049 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000002053: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei289 – 2891N6-(pyridoxal phosphate)lysineUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi580049.JALI_4331.

Family & Domainsi

Sequence similaritiesi

Belongs to the SHMT family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0112.
HOGENOMiHOG000239404.
KOiK00600.
OMAiAAWANVQ.
OrthoDBiEOG6Z0QB2.

Family and domain databases

Gene3Di3.40.640.10. 2 hits.
3.90.1150.10. 1 hit.
HAMAPiMF_00051. SHMT.
InterProiIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
PANTHERiPTHR11680. PTHR11680. 1 hit.
PfamiPF00464. SHMT. 2 hits.
[Graphical view]
PIRSFiPIRSF000412. SHMT. 1 hit.
SUPFAMiSSF53383. SSF53383. 2 hits.
PROSITEiPS00096. SHMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C4PRB0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASLLDRYLR NISDKSQQNL ASVAYSASLD HLLHAFPSIG QSIVQELKSQ
60 70 80 90 100
RSRLKMIASE NFSSLSVQLA MGNLLTDKYC EGSPFKRFYS CCENVDAIEW
110 120 130 140 150
ECAETAKELF GAESAFVQPH SGADANLLAI MSIITQKIQS PAVQRLGYKT
160 170 180 190 200
INDLPEQEYE ALKAEMAQHK CLGPSLNSGG HLTHGTVRMN IMSKLMHCLP
210 220 230 240 250
YEVNLDTELF DYDEIAKIAK EHKPTVLIAG YSSYSRRLNF ATLKQIAEDC
260 270 280 290 300
GAVLWVDMAH FAGLVAGGVF VGEENPMPYA DIVTTTTHKT LRGPRGGLVL
310 320 330 340 350
AKKEYANTLN KACPLMMGGP LPHVIAAKAI ALKEAMTINF RKYAHKVVEN
360 370 380 390 400
ARTLAEVFQR NGLRLLTGGT DNHMLIIDLT SLGVPGRIAE DMLTSVGIAV
410 420 430 440 450
NRNTIPSDAS GQWKTSGIRL GTPALTTLGM GSAEMEEVAN IIVKVLRNIT
460 470 480 490
VRSNAESGSS KSEGELSEGI AQEARQRVAD LLGRFPLYPE IDLETLV
Length:497
Mass (Da):54,245
Last modified:July 7, 2009 - v1
Checksum:i73663EF9F07AA11F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM872308 Genomic DNA. Translation: CAX10887.1.
RefSeqiYP_002888053.1. NC_012686.1.

Genome annotation databases

EnsemblBacteriaiCAX10887; CAX10887; JALI_4331.
GeneIDi7882273.
KEGGictj:JALI_4331.
PATRICi20378430. VBIChlTra100303_0488.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FM872308 Genomic DNA. Translation: CAX10887.1.
RefSeqiYP_002888053.1. NC_012686.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi580049.JALI_4331.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAX10887; CAX10887; JALI_4331.
GeneIDi7882273.
KEGGictj:JALI_4331.
PATRICi20378430. VBIChlTra100303_0488.

Phylogenomic databases

eggNOGiCOG0112.
HOGENOMiHOG000239404.
KOiK00600.
OMAiAAWANVQ.
OrthoDBiEOG6Z0QB2.

Enzyme and pathway databases

UniPathwayiUPA00193.
UPA00288; UER01023.
BioCyciCTRA580049:GH1T-458-MONOMER.

Family and domain databases

Gene3Di3.40.640.10. 2 hits.
3.90.1150.10. 1 hit.
HAMAPiMF_00051. SHMT.
InterProiIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
PANTHERiPTHR11680. PTHR11680. 1 hit.
PfamiPF00464. SHMT. 2 hits.
[Graphical view]
PIRSFiPIRSF000412. SHMT. 1 hit.
SUPFAMiSSF53383. SSF53383. 2 hits.
PROSITEiPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Jali20/OTImported.

Entry informationi

Entry nameiC4PRB0_CHLTJ
AccessioniPrimary (citable) accession number: C4PRB0
Entry historyi
Integrated into UniProtKB/TrEMBL: July 7, 2009
Last sequence update: July 7, 2009
Last modified: February 4, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.