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C4MBZ5

- PKS3_ALOAR

UniProt

C4MBZ5 - PKS3_ALOAR

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Protein
Aloesone synthase
Gene
PKS3
Organism
Aloe arborescens (Kidachi aloe)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the iterative condensations of 6, 7 or 8 molecules of malonyl-CoA to produce various aromatic polyketides. Produces the heptaketide aloesone, the aglycone of aloesin, from 7 molecules of malonyl-CoA as a major product. Also able to produce a hexaketide pyrone, a heptaketide 6-(2-acetyl-3,5-dihydroxybenzyl)-4-hydroxy-2-pyrone, a novel heptaketide 6-(2-(2,4-dihydroxy-6-methylphenyl)-2-oxoethyl)-4-hydroxy-2-pyrone and octaketides SEK4/SEK4b.1 Publication

Kineticsi

Kcat is 0.0075 min(-1).

  1. KM=88 µM for malonyl-CoA1 Publication

pH dependencei

Optimum pH is 6.0.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei174 – 1741 By similarity
Sitei207 – 2071Determines the polyketide chain length and product specificity
Binding sitei281 – 2811Coenzyme A By similarity

GO - Molecular functioni

  1. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB

GO - Biological processi

  1. flavonoid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Flavonoid biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15005.
UniPathwayiUPA00154.

Names & Taxonomyi

Protein namesi
Recommended name:
Aloesone synthase (EC:2.3.1.-)
Alternative name(s):
Polyketide synthase 3
Gene namesi
Name:PKS3
OrganismiAloe arborescens (Kidachi aloe)
Taxonomic identifieri45385 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaAsparagalesXanthorrhoeaceaeAsphodeloideaeAloe

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi207 – 2071A → G: Turns into a octaketide synthase. 1 Publication
Mutagenesisi207 – 2071A → M: Turns into a pentaketide synthase. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403Aloesone synthase
PRO_0000422576Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliC4MBZ5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni318 – 3214Coenzyme A binding By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.

Sequencei

Sequence statusi: Complete.

C4MBZ5-1 [UniParc]FASTAAdd to Basket

« Hide

MGSLSDSTPL MKDVQGIRKA QKADGTATVM AIGTAHPPHI ISQDSYADFY    50
FRVTNSEHKV ELKKKFDRIC KKTMIGKRYF NFDEEFLKKY PNITSFDKPS 100
LNDRHDICIP GVPALGAEAA VKAIEEWGRP KSEITHLVFC TSGGVDMPSA 150
DFQCAKLLGL RTNVNKYCIY MQGCYAGGTV MRYAKDLAEN NRGARVLMVC 200
AELTIIALRG PNDSHIDNAI GNSLFGDGAA ALIVGSDPII GVEKPMFEIV 250
CAKQTVIPNS EEVIHLHLRE SGLMFYMTKD SAATISNNIE ACLVDVFKSV 300
GMTPPEDWNS LFWIPHPGGR AILDQVEAKL KLRPEKFSAT RTVLWDYGNM 350
ISACVLYILD EMRRKSAAEG LETYGEGLEW GVLLGFGPGM TIETILLHSL 400
PPV 403
Length:403
Mass (Da):44,419
Last modified:July 7, 2009 - v1
Checksum:iA16E6D3309E0568F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF537574 mRNA. Translation: ABS72373.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF537574 mRNA. Translation: ABS72373.1 .

3D structure databases

ProteinModelPortali C4MBZ5.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00154 .
BioCyci MetaCyc:MONOMER-15005.

Family and domain databases

Gene3Di 3.40.47.10. 2 hits.
InterProi IPR012328. Chalcone/stilbene_synth_C.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000451. PKS_III. 1 hit.
SUPFAMi SSF53901. SSF53901. 2 hits.
ProtoNeti Search...

Publicationsi

  1. "Novel type III polyketide synthases from Aloe arborescens."
    Mizuuchi Y., Shi S.P., Wanibuchi K., Kojima A., Morita H., Noguchi H., Abe I.
    FEBS J. 276:2391-2401(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF ALA-207.

Entry informationi

Entry nameiPKS3_ALOAR
AccessioniPrimary (citable) accession number: C4MBZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2013
Last sequence update: July 7, 2009
Last modified: February 19, 2014
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

A.arborescens is a medicinal plant rich in aromatic polyketides, such as pharmaceutically important aloenin (hexaketide), aloesin (heptaketide) and barbaloin (octaketide).

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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