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Protein

Aloesone synthase

Gene

PKS3

Organism
Aloe arborescens (Kidachi aloe)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the iterative condensations of 6, 7 or 8 molecules of malonyl-CoA to produce various aromatic polyketides. Produces the heptaketide aloesone, the aglycone of aloesin, from 7 molecules of malonyl-CoA as a major product. Also able to produce a hexaketide pyrone, a heptaketide 6-(2-acetyl-3,5-dihydroxybenzyl)-4-hydroxy-2-pyrone, a novel heptaketide 6-(2-(2,4-dihydroxy-6-methylphenyl)-2-oxoethyl)-4-hydroxy-2-pyrone and octaketides SEK4/SEK4b.1 Publication

Kineticsi

Kcat is 0.0075 min(-1).

  1. KM=88 µM for malonyl-CoA1 Publication

pH dependencei

Optimum pH is 6.0.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei174 – 1741By similarity
Sitei207 – 2071Determines the polyketide chain length and product specificity
Binding sitei281 – 2811Coenzyme ABy similarity

GO - Molecular functioni

  1. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB

GO - Biological processi

  1. flavonoid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Flavonoid biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15005.
UniPathwayiUPA00154.

Names & Taxonomyi

Protein namesi
Recommended name:
Aloesone synthase (EC:2.3.1.-)
Alternative name(s):
Polyketide synthase 3
Gene namesi
Name:PKS3
OrganismiAloe arborescens (Kidachi aloe)
Taxonomic identifieri45385 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaAsparagalesXanthorrhoeaceaeAsphodeloideaeAloe

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi207 – 2071A → G: Turns into a octaketide synthase. 1 Publication
Mutagenesisi207 – 2071A → M: Turns into a pentaketide synthase. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403Aloesone synthasePRO_0000422576Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliC4MBZ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni318 – 3214Coenzyme A bindingBy similarity

Sequence similaritiesi

Belongs to the chalcone/stilbene synthases family.Curated

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.

Sequencei

Sequence statusi: Complete.

C4MBZ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSLSDSTPL MKDVQGIRKA QKADGTATVM AIGTAHPPHI ISQDSYADFY
60 70 80 90 100
FRVTNSEHKV ELKKKFDRIC KKTMIGKRYF NFDEEFLKKY PNITSFDKPS
110 120 130 140 150
LNDRHDICIP GVPALGAEAA VKAIEEWGRP KSEITHLVFC TSGGVDMPSA
160 170 180 190 200
DFQCAKLLGL RTNVNKYCIY MQGCYAGGTV MRYAKDLAEN NRGARVLMVC
210 220 230 240 250
AELTIIALRG PNDSHIDNAI GNSLFGDGAA ALIVGSDPII GVEKPMFEIV
260 270 280 290 300
CAKQTVIPNS EEVIHLHLRE SGLMFYMTKD SAATISNNIE ACLVDVFKSV
310 320 330 340 350
GMTPPEDWNS LFWIPHPGGR AILDQVEAKL KLRPEKFSAT RTVLWDYGNM
360 370 380 390 400
ISACVLYILD EMRRKSAAEG LETYGEGLEW GVLLGFGPGM TIETILLHSL

PPV
Length:403
Mass (Da):44,419
Last modified:July 7, 2009 - v1
Checksum:iA16E6D3309E0568F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF537574 mRNA. Translation: ABS72373.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF537574 mRNA. Translation: ABS72373.1.

3D structure databases

ProteinModelPortaliC4MBZ5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00154.
BioCyciMetaCyc:MONOMER-15005.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "Novel type III polyketide synthases from Aloe arborescens."
    Mizuuchi Y., Shi S.P., Wanibuchi K., Kojima A., Morita H., Noguchi H., Abe I.
    FEBS J. 276:2391-2401(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF ALA-207.

Entry informationi

Entry nameiPKS3_ALOAR
AccessioniPrimary (citable) accession number: C4MBZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2013
Last sequence update: July 7, 2009
Last modified: October 1, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

A.arborescens is a medicinal plant rich in aromatic polyketides, such as pharmaceutically important aloenin (hexaketide), aloesin (heptaketide) and barbaloin (octaketide).

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.