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C4MBZ5 (PKS3_ALOAR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aloesone synthase

EC=2.3.1.-
Alternative name(s):
Polyketide synthase 3
Gene names
Name:PKS3
OrganismAloe arborescens (Kidachi aloe)
Taxonomic identifier45385 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaAsparagalesXanthorrhoeaceaeAsphodeloideaeAloe

Protein attributes

Sequence length403 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the iterative condensations of 6, 7 or 8 molecules of malonyl-CoA to produce various aromatic polyketides. Produces the heptaketide aloesone, the aglycone of aloesin, from 7 molecules of malonyl-CoA as a major product. Also able to produce a hexaketide pyrone, a heptaketide 6-(2-acetyl-3,5-dihydroxybenzyl)-4-hydroxy-2-pyrone, a novel heptaketide 6-(2-(2,4-dihydroxy-6-methylphenyl)-2-oxoethyl)-4-hydroxy-2-pyrone and octaketides SEK4/SEK4b. Ref.1

Pathway

Secondary metabolite biosynthesis; flavonoid biosynthesis.

Subunit structure

Homodimer By similarity.

Miscellaneous

A.arborescens is a medicinal plant rich in aromatic polyketides, such as pharmaceutically important aloenin (hexaketide), aloesin (heptaketide) and barbaloin (octaketide).

Sequence similarities

Belongs to the chalcone/stilbene synthases family.

Biophysicochemical properties

Kinetic parameters:

Kcat is 0.0075 min(-1).

KM=88 µM for malonyl-CoA Ref.1

pH dependence:

Optimum pH is 6.0.

Ontologies

Keywords
   Biological processFlavonoid biosynthesis
   Molecular functionAcyltransferase
Transferase
Gene Ontology (GO)
   Biological_processflavonoid biosynthetic process

Inferred from direct assay Ref.1. Source: UniProtKB

   Molecular_functiontransferase activity, transferring acyl groups other than amino-acyl groups

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 403403Aloesone synthase
PRO_0000422576

Regions

Region318 – 3214Coenzyme A binding By similarity

Sites

Active site1741 By similarity
Binding site2811Coenzyme A By similarity
Site2071Determines the polyketide chain length and product specificity

Experimental info

Mutagenesis2071A → G: Turns into a octaketide synthase. Ref.1
Mutagenesis2071A → M: Turns into a pentaketide synthase. Ref.1

Sequences

Sequence LengthMass (Da)Tools
C4MBZ5 [UniParc].

Last modified July 7, 2009. Version 1.
Checksum: A16E6D3309E0568F

FASTA40344,419
        10         20         30         40         50         60 
MGSLSDSTPL MKDVQGIRKA QKADGTATVM AIGTAHPPHI ISQDSYADFY FRVTNSEHKV 

        70         80         90        100        110        120 
ELKKKFDRIC KKTMIGKRYF NFDEEFLKKY PNITSFDKPS LNDRHDICIP GVPALGAEAA 

       130        140        150        160        170        180 
VKAIEEWGRP KSEITHLVFC TSGGVDMPSA DFQCAKLLGL RTNVNKYCIY MQGCYAGGTV 

       190        200        210        220        230        240 
MRYAKDLAEN NRGARVLMVC AELTIIALRG PNDSHIDNAI GNSLFGDGAA ALIVGSDPII 

       250        260        270        280        290        300 
GVEKPMFEIV CAKQTVIPNS EEVIHLHLRE SGLMFYMTKD SAATISNNIE ACLVDVFKSV 

       310        320        330        340        350        360 
GMTPPEDWNS LFWIPHPGGR AILDQVEAKL KLRPEKFSAT RTVLWDYGNM ISACVLYILD 

       370        380        390        400 
EMRRKSAAEG LETYGEGLEW GVLLGFGPGM TIETILLHSL PPV 

« Hide

References

[1]"Novel type III polyketide synthases from Aloe arborescens."
Mizuuchi Y., Shi S.P., Wanibuchi K., Kojima A., Morita H., Noguchi H., Abe I.
FEBS J. 276:2391-2401(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF ALA-207.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF537574 mRNA. Translation: ABS72373.1.

3D structure databases

ProteinModelPortalC4MBZ5.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-15005.
UniPathwayUPA00154.

Family and domain databases

Gene3D3.40.47.10. 2 hits.
InterProIPR012328. Chalcone/stilbene_synth_C.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFPIRSF000451. PKS_III. 1 hit.
SUPFAMSSF53901. SSF53901. 2 hits.
ProtoNetSearch...

Entry information

Entry namePKS3_ALOAR
AccessionPrimary (citable) accession number: C4MBZ5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2013
Last sequence update: July 7, 2009
Last modified: February 19, 2014
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways