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Protein

Aloesone synthase

Gene

PKS3

Organism
Aloe arborescens (Kidachi aloe)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the iterative condensations of 6, 7 or 8 molecules of malonyl-CoA to produce various aromatic polyketides. Produces the heptaketide aloesone, the aglycone of aloesin, from 7 molecules of malonyl-CoA as a major product. Also able to produce a hexaketide pyrone, a heptaketide 6-(2-acetyl-3,5-dihydroxybenzyl)-4-hydroxy-2-pyrone, a novel heptaketide 6-(2-(2,4-dihydroxy-6-methylphenyl)-2-oxoethyl)-4-hydroxy-2-pyrone and octaketides SEK4/SEK4b.1 Publication

Kineticsi

Kcat is 0.0075 min(-1).

  1. KM=88 µM for malonyl-CoA1 Publication

    pH dependencei

    Optimum pH is 6.0.1 Publication

    Pathway:iflavonoid biosynthesis

    This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei174 – 1741By similarity
    Sitei207 – 2071Determines the polyketide chain length and product specificity
    Binding sitei281 – 2811Coenzyme ABy similarity

    GO - Molecular functioni

    GO - Biological processi

    • flavonoid biosynthetic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Flavonoid biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15005.
    UniPathwayiUPA00154.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aloesone synthase (EC:2.3.1.-)
    Alternative name(s):
    Polyketide synthase 3
    Gene namesi
    Name:PKS3
    OrganismiAloe arborescens (Kidachi aloe)
    Taxonomic identifieri45385 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaAsparagalesXanthorrhoeaceaeAsphodeloideaeAloe

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi207 – 2071A → G: Turns into a octaketide synthase. 1 Publication
    Mutagenesisi207 – 2071A → M: Turns into a pentaketide synthase. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 403403Aloesone synthasePRO_0000422576Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliC4MBZ5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni318 – 3214Coenzyme A bindingBy similarity

    Sequence similaritiesi

    Belongs to the chalcone/stilbene synthases family.Curated

    Family and domain databases

    Gene3Di3.40.47.10. 2 hits.
    InterProiIPR012328. Chalcone/stilbene_synth_C.
    IPR001099. Chalcone/stilbene_synthase_N.
    IPR011141. Polyketide_synthase_type-III.
    IPR016039. Thiolase-like.
    [Graphical view]
    PfamiPF02797. Chal_sti_synt_C. 1 hit.
    PF00195. Chal_sti_synt_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000451. PKS_III. 1 hit.
    SUPFAMiSSF53901. SSF53901. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    C4MBZ5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGSLSDSTPL MKDVQGIRKA QKADGTATVM AIGTAHPPHI ISQDSYADFY
    60 70 80 90 100
    FRVTNSEHKV ELKKKFDRIC KKTMIGKRYF NFDEEFLKKY PNITSFDKPS
    110 120 130 140 150
    LNDRHDICIP GVPALGAEAA VKAIEEWGRP KSEITHLVFC TSGGVDMPSA
    160 170 180 190 200
    DFQCAKLLGL RTNVNKYCIY MQGCYAGGTV MRYAKDLAEN NRGARVLMVC
    210 220 230 240 250
    AELTIIALRG PNDSHIDNAI GNSLFGDGAA ALIVGSDPII GVEKPMFEIV
    260 270 280 290 300
    CAKQTVIPNS EEVIHLHLRE SGLMFYMTKD SAATISNNIE ACLVDVFKSV
    310 320 330 340 350
    GMTPPEDWNS LFWIPHPGGR AILDQVEAKL KLRPEKFSAT RTVLWDYGNM
    360 370 380 390 400
    ISACVLYILD EMRRKSAAEG LETYGEGLEW GVLLGFGPGM TIETILLHSL

    PPV
    Length:403
    Mass (Da):44,419
    Last modified:July 7, 2009 - v1
    Checksum:iA16E6D3309E0568F
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    EF537574 mRNA. Translation: ABS72373.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    EF537574 mRNA. Translation: ABS72373.1.

    3D structure databases

    ProteinModelPortaliC4MBZ5.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    UniPathwayiUPA00154.
    BioCyciMetaCyc:MONOMER-15005.

    Family and domain databases

    Gene3Di3.40.47.10. 2 hits.
    InterProiIPR012328. Chalcone/stilbene_synth_C.
    IPR001099. Chalcone/stilbene_synthase_N.
    IPR011141. Polyketide_synthase_type-III.
    IPR016039. Thiolase-like.
    [Graphical view]
    PfamiPF02797. Chal_sti_synt_C. 1 hit.
    PF00195. Chal_sti_synt_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000451. PKS_III. 1 hit.
    SUPFAMiSSF53901. SSF53901. 2 hits.
    ProtoNetiSearch...

    Publicationsi

    1. "Novel type III polyketide synthases from Aloe arborescens."
      Mizuuchi Y., Shi S.P., Wanibuchi K., Kojima A., Morita H., Noguchi H., Abe I.
      FEBS J. 276:2391-2401(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF ALA-207.

    Entry informationi

    Entry nameiPKS3_ALOAR
    AccessioniPrimary (citable) accession number: C4MBZ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2013
    Last sequence update: July 7, 2009
    Last modified: July 22, 2015
    This is version 23 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    A.arborescens is a medicinal plant rich in aromatic polyketides, such as pharmaceutically important aloenin (hexaketide), aloesin (heptaketide) and barbaloin (octaketide).

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.