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C4MBZ5

- PKS3_ALOAR

UniProt

C4MBZ5 - PKS3_ALOAR

Protein

Aloesone synthase

Gene

PKS3

Organism
Aloe arborescens (Kidachi aloe)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 22 (01 Oct 2014)
      Sequence version 1 (07 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the iterative condensations of 6, 7 or 8 molecules of malonyl-CoA to produce various aromatic polyketides. Produces the heptaketide aloesone, the aglycone of aloesin, from 7 molecules of malonyl-CoA as a major product. Also able to produce a hexaketide pyrone, a heptaketide 6-(2-acetyl-3,5-dihydroxybenzyl)-4-hydroxy-2-pyrone, a novel heptaketide 6-(2-(2,4-dihydroxy-6-methylphenyl)-2-oxoethyl)-4-hydroxy-2-pyrone and octaketides SEK4/SEK4b.1 Publication

    Kineticsi

    Kcat is 0.0075 min(-1).

    1. KM=88 µM for malonyl-CoA1 Publication

    pH dependencei

    Optimum pH is 6.0.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei174 – 1741By similarity
    Sitei207 – 2071Determines the polyketide chain length and product specificity
    Binding sitei281 – 2811Coenzyme ABy similarity

    GO - Molecular functioni

    1. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB

    GO - Biological processi

    1. flavonoid biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Flavonoid biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15005.
    UniPathwayiUPA00154.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aloesone synthase (EC:2.3.1.-)
    Alternative name(s):
    Polyketide synthase 3
    Gene namesi
    Name:PKS3
    OrganismiAloe arborescens (Kidachi aloe)
    Taxonomic identifieri45385 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaAsparagalesXanthorrhoeaceaeAsphodeloideaeAloe

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi207 – 2071A → G: Turns into a octaketide synthase. 1 Publication
    Mutagenesisi207 – 2071A → M: Turns into a pentaketide synthase. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 403403Aloesone synthasePRO_0000422576Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliC4MBZ5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni318 – 3214Coenzyme A bindingBy similarity

    Sequence similaritiesi

    Belongs to the chalcone/stilbene synthases family.Curated

    Family and domain databases

    Gene3Di3.40.47.10. 2 hits.
    InterProiIPR012328. Chalcone/stilbene_synth_C.
    IPR001099. Chalcone/stilbene_synthase_N.
    IPR011141. Polyketide_synthase_type-III.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF02797. Chal_sti_synt_C. 1 hit.
    PF00195. Chal_sti_synt_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000451. PKS_III. 1 hit.
    SUPFAMiSSF53901. SSF53901. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    C4MBZ5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSLSDSTPL MKDVQGIRKA QKADGTATVM AIGTAHPPHI ISQDSYADFY    50
    FRVTNSEHKV ELKKKFDRIC KKTMIGKRYF NFDEEFLKKY PNITSFDKPS 100
    LNDRHDICIP GVPALGAEAA VKAIEEWGRP KSEITHLVFC TSGGVDMPSA 150
    DFQCAKLLGL RTNVNKYCIY MQGCYAGGTV MRYAKDLAEN NRGARVLMVC 200
    AELTIIALRG PNDSHIDNAI GNSLFGDGAA ALIVGSDPII GVEKPMFEIV 250
    CAKQTVIPNS EEVIHLHLRE SGLMFYMTKD SAATISNNIE ACLVDVFKSV 300
    GMTPPEDWNS LFWIPHPGGR AILDQVEAKL KLRPEKFSAT RTVLWDYGNM 350
    ISACVLYILD EMRRKSAAEG LETYGEGLEW GVLLGFGPGM TIETILLHSL 400
    PPV 403
    Length:403
    Mass (Da):44,419
    Last modified:July 7, 2009 - v1
    Checksum:iA16E6D3309E0568F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF537574 mRNA. Translation: ABS72373.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF537574 mRNA. Translation: ABS72373.1 .

    3D structure databases

    ProteinModelPortali C4MBZ5.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00154 .
    BioCyci MetaCyc:MONOMER-15005.

    Family and domain databases

    Gene3Di 3.40.47.10. 2 hits.
    InterProi IPR012328. Chalcone/stilbene_synth_C.
    IPR001099. Chalcone/stilbene_synthase_N.
    IPR011141. Polyketide_synthase_type-III.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF02797. Chal_sti_synt_C. 1 hit.
    PF00195. Chal_sti_synt_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000451. PKS_III. 1 hit.
    SUPFAMi SSF53901. SSF53901. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Novel type III polyketide synthases from Aloe arborescens."
      Mizuuchi Y., Shi S.P., Wanibuchi K., Kojima A., Morita H., Noguchi H., Abe I.
      FEBS J. 276:2391-2401(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF ALA-207.

    Entry informationi

    Entry nameiPKS3_ALOAR
    AccessioniPrimary (citable) accession number: C4MBZ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2013
    Last sequence update: July 7, 2009
    Last modified: October 1, 2014
    This is version 22 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    A.arborescens is a medicinal plant rich in aromatic polyketides, such as pharmaceutically important aloenin (hexaketide), aloesin (heptaketide) and barbaloin (octaketide).

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3