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C4M1C3

- C4M1C3_ENTHI

UniProt

C4M1C3 - C4M1C3_ENTHI

Protein

Probable acetate kinase

Gene

EHI_170010

Organism
Entamoeba histolytica
Status
Unreviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (07 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + acetate = ADP + acetyl phosphate.UniRule annotation

    Cofactori

    Mg2+.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi8 – 81MagnesiumUniRule annotation
    Binding sitei85 – 851SubstrateUniRule annotation
    Active sitei142 – 1421Proton donor/acceptorUniRule annotation
    Sitei172 – 1721Transition state stabilizerUniRule annotation
    Sitei232 – 2321Transition state stabilizerUniRule annotation
    Metal bindingi378 – 3781MagnesiumUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi272 – 2743ATPUniRule annotation

    GO - Molecular functioni

    1. acetate kinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. acetyl-CoA biosynthetic process Source: UniProtKB-UniPathway
    2. organic acid metabolic process Source: InterPro

    Keywords - Molecular functioni

    KinaseUniRule annotationImported, Transferase

    Keywords - Ligandi

    ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00340; UER00458.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable acetate kinaseUniRule annotation (EC:2.7.2.1UniRule annotation)
    Alternative name(s):
    AcetokinaseUniRule annotation
    Gene namesi
    ORF Names:EHI_170010Imported
    OrganismiEntamoeba histolyticaImported
    Taxonomic identifieri5759 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaArchamoebaeEntamoebidaeEntamoeba
    ProteomesiUP000001926: Partially assembled WGS sequence

    Organism-specific databases

    EuPathDBiAmoebaDB:EHI_170010.

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    Interactioni

    Protein-protein interaction databases

    STRINGi294381.C4M1C3.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4H0OX-ray2.40A/B1-392[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the acetokinase family.UniRule annotation

    Phylogenomic databases

    KOiK00925.

    Family and domain databases

    HAMAPiMF_00020. Acetate_kinase.
    InterProiIPR004372. Ac/Proprionate_kinase.
    IPR000890. Aliphatic_acid_kin_short-chain.
    [Graphical view]
    PANTHERiPTHR21060. PTHR21060. 1 hit.
    PfamiPF00871. Acetate_kinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
    PRINTSiPR00471. ACETATEKNASE.
    TIGRFAMsiTIGR00016. ackA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    C4M1C3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNVLIFNVG SSSLTYKVFC SDNIVCSGKS NRVNVTGTEK PFIEHHLNGQ    50
    IIKIETPILN HPQAAKLIIQ FLKENHISIA FVGHRFVHGG SYFKKSAVID 100
    EVVLKELKEC LPLAPIHNPS SFGVIEISMK ELPTTRQYVA IDTAFHSTIS 150
    QAERTYAIPQ PYQSQYLKFG FHGLSYEYVI NSLKNVIDVS HSKIIACHLG 200
    TGGSSCCGIV NGKSFDTSMG NSTLAGLVMS TRCGDIDPTI PIDMIQQVGI 250
    EKVVDILNKK SGLLGVSELS SDMRDILHEI ETRGPKAKTC QLAFDVYIKQ 300
    LAKTIGGLMV EIGGLDLLVF TDQMGLEVWQ VRKAICDKMK FLGIELDDSL 350
    NEKSMGKKIE FLTMPSSKVQ VCVAPNDEEL VILQKGKELF QF 392
    Length:392
    Mass (Da):43,247
    Last modified:July 7, 2009 - v1
    Checksum:i55ED2FE3B0836FF1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS571207 Genomic DNA. Translation: EAL50605.1.
    RefSeqiXP_655990.1. XM_650898.1.

    Genome annotation databases

    EnsemblProtistsirna_EHI_170010-1; rna_EHI_170010-1; EHI_170010.
    GeneIDi3410291.
    KEGGiehi:EHI_170010.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS571207 Genomic DNA. Translation: EAL50605.1 .
    RefSeqi XP_655990.1. XM_650898.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4H0O X-ray 2.40 A/B 1-392 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 294381.C4M1C3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi rna_EHI_170010-1 ; rna_EHI_170010-1 ; EHI_170010 .
    GeneIDi 3410291.
    KEGGi ehi:EHI_170010.

    Organism-specific databases

    EuPathDBi AmoebaDB:EHI_170010.

    Phylogenomic databases

    KOi K00925.

    Enzyme and pathway databases

    UniPathwayi UPA00340 ; UER00458 .

    Family and domain databases

    HAMAPi MF_00020. Acetate_kinase.
    InterProi IPR004372. Ac/Proprionate_kinase.
    IPR000890. Aliphatic_acid_kin_short-chain.
    [Graphical view ]
    PANTHERi PTHR21060. PTHR21060. 1 hit.
    Pfami PF00871. Acetate_kinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000722. Acetate_prop_kin. 1 hit.
    PRINTSi PR00471. ACETATEKNASE.
    TIGRFAMsi TIGR00016. ackA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 30459 / HM-1:IMSSImported.
    2. Lorenzi H., Amedeo P., Inman J., Schobel S., Caler E.
      Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: ATCC 30459 / HM-1:IMSSImported.
    3. "Crystal structures of acetate kinases from the eukaryotic pathogens Entamoeba histolytica and Cryptococcus neoformans."
      Thaker T.M., Tanabe M., Fowler M.L., Preininger A.M., Ingram-Smith C., Smith K.S., Iverson T.M.
      J. Struct. Biol. 181:185-189(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS).

    Entry informationi

    Entry nameiC4M1C3_ENTHI
    AccessioniPrimary (citable) accession number: C4M1C3
    Entry historyi
    Integrated into UniProtKB/TrEMBL: July 7, 2009
    Last sequence update: July 7, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3