Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable acetate kinase

Gene

EHI_170010

Organism
Entamoeba histolytica
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + acetate = ADP + acetyl phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathway: acetyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes acetyl-CoA from acetate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable acetate kinase (EHI_170010)
  2. no protein annotated in this organism
This subpathway is part of the pathway acetyl-CoA biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from acetate, the pathway acetyl-CoA biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81MagnesiumUniRule annotation
Binding sitei85 – 851SubstrateUniRule annotation
Active sitei142 – 1421Proton donor/acceptorUniRule annotation
Sitei172 – 1721Transition state stabilizerUniRule annotation
Sitei232 – 2321Transition state stabilizerUniRule annotation
Metal bindingi378 – 3781MagnesiumUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi272 – 2743ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationImported, Transferase

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.2.1. 2080.
UniPathwayiUPA00340; UER00458.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable acetate kinaseUniRule annotation (EC:2.7.2.1UniRule annotation)
Alternative name(s):
AcetokinaseUniRule annotation
Gene namesi
ORF Names:EHI_170010Imported
OrganismiEntamoeba histolyticaImported
Taxonomic identifieri5759 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaArchamoebaeEntamoebidaeEntamoeba
ProteomesiUP000001926 Componenti: Partially assembled WGS sequence

Organism-specific databases

EuPathDBiAmoebaDB:EHI_170010.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Interactioni

Protein-protein interaction databases

STRINGi5759.rna_EHI_170010-1.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4H0OX-ray2.40A/B1-392[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acetokinase family.UniRule annotation

Phylogenomic databases

InParanoidiC4M1C3.
KOiK00925.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase.
InterProiIPR004372. Ac/Proprionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
[Graphical view]
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiPF00871. Acetate_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.

Sequencei

Sequence statusi: Complete.

C4M1C3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNVLIFNVG SSSLTYKVFC SDNIVCSGKS NRVNVTGTEK PFIEHHLNGQ
60 70 80 90 100
IIKIETPILN HPQAAKLIIQ FLKENHISIA FVGHRFVHGG SYFKKSAVID
110 120 130 140 150
EVVLKELKEC LPLAPIHNPS SFGVIEISMK ELPTTRQYVA IDTAFHSTIS
160 170 180 190 200
QAERTYAIPQ PYQSQYLKFG FHGLSYEYVI NSLKNVIDVS HSKIIACHLG
210 220 230 240 250
TGGSSCCGIV NGKSFDTSMG NSTLAGLVMS TRCGDIDPTI PIDMIQQVGI
260 270 280 290 300
EKVVDILNKK SGLLGVSELS SDMRDILHEI ETRGPKAKTC QLAFDVYIKQ
310 320 330 340 350
LAKTIGGLMV EIGGLDLLVF TDQMGLEVWQ VRKAICDKMK FLGIELDDSL
360 370 380 390
NEKSMGKKIE FLTMPSSKVQ VCVAPNDEEL VILQKGKELF QF
Length:392
Mass (Da):43,247
Last modified:July 7, 2009 - v1
Checksum:i55ED2FE3B0836FF1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS571207 Genomic DNA. Translation: EAL50605.1.
RefSeqiXP_655990.1. XM_650898.1.

Genome annotation databases

EnsemblProtistsirna_EHI_170010-1; rna_EHI_170010-1; EHI_170010.
GeneIDi3410291.
KEGGiehi:EHI_170010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS571207 Genomic DNA. Translation: EAL50605.1.
RefSeqiXP_655990.1. XM_650898.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4H0OX-ray2.40A/B1-392[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5759.rna_EHI_170010-1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsirna_EHI_170010-1; rna_EHI_170010-1; EHI_170010.
GeneIDi3410291.
KEGGiehi:EHI_170010.

Organism-specific databases

EuPathDBiAmoebaDB:EHI_170010.

Phylogenomic databases

InParanoidiC4M1C3.
KOiK00925.

Enzyme and pathway databases

UniPathwayiUPA00340; UER00458.
BRENDAi2.7.2.1. 2080.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase.
InterProiIPR004372. Ac/Proprionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
[Graphical view]
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiPF00871. Acetate_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 30459 / HM-1:IMSSImported.
  2. Lorenzi H., Amedeo P., Inman J., Schobel S., Caler E.
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: ATCC 30459 / HM-1:IMSSImported.
  3. "Crystal structures of acetate kinases from the eukaryotic pathogens Entamoeba histolytica and Cryptococcus neoformans."
    Thaker T.M., Tanabe M., Fowler M.L., Preininger A.M., Ingram-Smith C., Smith K.S., Iverson T.M.
    J. Struct. Biol. 181:185-189(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS).

Entry informationi

Entry nameiC4M1C3_ENTHI
AccessioniPrimary (citable) accession number: C4M1C3
Entry historyi
Integrated into UniProtKB/TrEMBL: July 7, 2009
Last sequence update: July 7, 2009
Last modified: June 24, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.