C4LLJ1 (C4LLJ1_CORK4) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
DNA topoisomerase I EMBL ACR18696.1
EC=5.99.1.2 EMBL ACR18696.1

Gene names

Ordered Locus Names:

ckrop_1988

Organism

Corynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717) [Complete proteome] [HAMAP]

Taxonomic identifier

645127 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	987 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733
Sequence similarities	Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	C4LLJ1 [UniParc]. Last modified July 7, 2009. Version 1. Checksum: 9D8849F8C5D3A3F3	FASTA	987	108,500
	10 20 30 40 50 60 MKRLVIVESA TKARKIQPYL GSDYIVEASV GHIRDLPRGA ADVPAKYKKK PWARLGVDVE 70 80 90 100 110 120 NDFAPLYVVN QDKKKKVADL KAKLKQVDEL YLATDPDREG EAIAWHLLQV LKPKVPVRRM 130 140 150 160 170 180 VFHEITKPAI LEAAKHTREL DQNLVNAQET RRVLDRLYGY EISPVLWKKV MPRLSAGRVQ 190 200 210 220 230 240 SVATRVIVER ERDRIAFTSA DYWDLTGTFA LRSGADASTP GSSTAGADTA SGEPRSFPAR 250 260 270 280 290 300 LAEIDGQRVA QGRDFNDDGQ LKSKAVALDE KAAKDLADQL SGAAMSVRSV EEKPYTRRPY 310 320 330 340 350 360 PPFMTSTLQQ EAGRKLHYTS ERTMRIAQRL YENGHITYMR TDSTTLSESG INAARAQARE 370 380 390 400 410 420 LYGAKYVASA PRQYSRKVKN SQEAHEAIRP AGESFATPGQ LGGQLDAEEF KLYELIWQRT 430 440 450 460 470 480 VASQMADAKG TSMKVFVAGE STDGRDCVFT ATGRTITFPG FLKAYVEVSR TADGRNVADD 490 500 510 520 530 540 AEKRLPVLTE GQGLTATDIE ADGHSTTPPA RYTEASLVKK MEDLGIGRPS TYASIIKTIQ 550 560 570 580 590 600 DRGYVYSRGN ALVPSWVAFA VVGLLEQNFG SLIDYDFTSS MEDVLDSIAN GSEDRSHWLE 610 620 630 640 650 660 GFYYGDDGAS SAKAETIARL GGLKHLVGGN LETIDARAVN SLHMFDDEDG NPIVVRVGRY 670 680 690 700 710 720 GPYLERTPAG SDEPQRANLP DTVTPDELDL ALAEKLLATP PTGRELGENP ANGRMVVAKE 730 740 750 760 770 780 GRYGPYVTEL VRDDEREKAE AKALETVDAE WDADDEARKA EGKKPLNRET KTAAKKREKR 790 800 810 820 830 840 VNELVTEMLK PKTASLFSGM EPSTVTLDEA LKLMSLPREV GVDPSDGEVI TAQNGRYGPY 850 860 870 880 890 900 LKKGTDSRSL ANEEQIFTIT LDEARRIYAE PKRRGRAAAK PPLKQLGDND VSGKPMTVKE 910 920 930 940 950 960 GRFGPYVTDG ETNASLRRGD TPEAMTDARA CELLSERRAK EAANGGAKKK SSKKGTRKST 970 980 KKSGRKSTSK ASKKTSKKSP AKKTVKS

References

[1]

"Ultrafast pyrosequencing of Corynebacterium kroppenstedtii DSM44385 revealed insights into the physiology of a lipophilic corynebacterium that lacks mycolic acids." Tauch A., Schneider J., Szczepanowski R., Tilker A., Viehoever P., Gartemann K.-H., Arnold W., Blom J., Brinkrolf K., Brune I., Goetker S., Weisshaar B., Goesmann A., Droege M., Puehler A. J. Biotechnol. 136:22-30(2008) [PubMed: 18430482] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44385 / CCUG 35717.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001620 Genomic DNA. Translation: ACR18696.1.
RefSeq	YP_002907239.1. NC_012704.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	C4LLJ1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7876584.
GenomeReviews	Gene locus ckrop_1988 in contig CP001620_GR.
KEGG	ckp:ckrop_1988.
PATRIC	21519399. VBICorKro120627_2048.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KKVMPRL.
ProtClustDB	PRK07561.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C4LLJ1_CORK4

Accession

Primary (citable) accession number: C4LLJ1

Entry history

Integrated into UniProtKB/TrEMBL:	July 7, 2009
Last sequence update:	July 7, 2009
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)