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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Corynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 3711D-inositol 3-phosphateUniRule annotation
Binding sitei51 – 511UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei106 – 10611D-inositol 3-phosphateUniRule annotation
Binding sitei138 – 13811D-inositol 3-phosphateUniRule annotation
Binding sitei162 – 16211D-inositol 3-phosphateUniRule annotation
Binding sitei182 – 18211D-inositol 3-phosphateUniRule annotation
Binding sitei259 – 2591UDP-GlcNAcUniRule annotation
Binding sitei264 – 2641UDP-GlcNAcUniRule annotation
Binding sitei323 – 3231UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi332 – 3321Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi333 – 3331Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi335 – 3351Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei345 – 3451UDP-GlcNAcUniRule annotation
Binding sitei353 – 3531UDP-GlcNAcUniRule annotation
Metal bindingi359 – 3591MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciCKRO645127:GI7D-1902-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:ckrop_1927
OrganismiCorynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717)
Taxonomic identifieri645127 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000001473 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 451451D-inositol 3-phosphate glycosyltransferasePRO_0000400120Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi645127.ckrop_1927.

Structurei

3D structure databases

ProteinModelPortaliC4LLD6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni43 – 442UDP-GlcNAc bindingUniRule annotation
Regioni48 – 5361D-inositol 3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiEOG647TVR.

Family and domain databases

HAMAPiMF_01695. MshA.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

C4LLD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHQAGASTRR LHGVAILSTL KSMSTTPRGR IAVISMHTSP IEQPGVGDAG
60 70 80 90 100
GMNVYIRSTS LELGALGYEV DIFTRATRSS QGKIVQLAPN VRLINIIAGP
110 120 130 140 150
YEGLSKEELP TQMVAFTSGI IEFAQCEKVS YSLIHSHYWM SGQVGWLLRD
160 170 180 190 200
LWRVPQVHTA HTLALVKNSA LATGDRPEPE SRRICEQQIV DNADRLVVNT
210 220 230 240 250
EAGKDNLVFH YDADPEHIDV VLPGADVTQF SPGSDRATER SRRELGVPLH
260 270 280 290 300
ATVIAFVGRM QRLKGPQVLL RAVANMMKKH PDQELRVLMC GGPSGNGLAR
310 320 330 340 350
PTEFEDLARD LGIDPIVRFL APRPPEDLAS VYRAADIVAI PSYNESFGLV
360 370 380 390 400
AVEAQASGTP VVAARAGGLP ITIDDGTSGI LVDGHDPADW ATALQSLCDD
410 420 430 440 450
DDRRIAMGEN ATDHASRFSW ASSARHLSDI YEDAIRKGPH VRCGSDRAGA

S
Length:451
Mass (Da):48,799
Last modified:July 7, 2009 - v1
Checksum:i6792389B2B8426F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001620 Genomic DNA. Translation: ACR18641.1.

Genome annotation databases

EnsemblBacteriaiACR18641; ACR18641; ckrop_1927.
KEGGickp:ckrop_1927.
PATRICi21519269. VBICorKro120627_1984.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001620 Genomic DNA. Translation: ACR18641.1.

3D structure databases

ProteinModelPortaliC4LLD6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi645127.ckrop_1927.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACR18641; ACR18641; ckrop_1927.
KEGGickp:ckrop_1927.
PATRICi21519269. VBICorKro120627_1984.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiEOG647TVR.

Enzyme and pathway databases

BioCyciCKRO645127:GI7D-1902-MONOMER.

Family and domain databases

HAMAPiMF_01695. MshA.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Ultrafast pyrosequencing of Corynebacterium kroppenstedtii DSM44385 revealed insights into the physiology of a lipophilic corynebacterium that lacks mycolic acids."
    Tauch A., Schneider J., Szczepanowski R., Tilker A., Viehoever P., Gartemann K.-H., Arnold W., Blom J., Brinkrolf K., Brune I., Goetker S., Weisshaar B., Goesmann A., Droege M., Puehler A.
    J. Biotechnol. 136:22-30(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 44385 / CCUG 35717.

Entry informationi

Entry nameiMSHA_CORK4
AccessioniPrimary (citable) accession number: C4LLD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: July 7, 2009
Last modified: May 11, 2016
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.