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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Corynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi567 – 5671MagnesiumUniRule annotation
Metal bindingi573 – 5731MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciCKRO645127:GI7D-1123-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:ckrop_1133
OrganismiCorynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717)
Taxonomic identifieri645127 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000001473: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 775775Polyribonucleotide nucleotidyltransferasePRO_0000382421Add
BLAST

Proteomic databases

PRIDEiC4LJ74.

Interactioni

Protein-protein interaction databases

STRINGi645127.ckrop_1133.

Structurei

3D structure databases

ProteinModelPortaliC4LJ74.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini633 – 69260KHUniRule annotationAdd
BLAST
Domaini704 – 77370S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C4LJ74-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIARGTHREG RIPPFMTNNH SVFYDEEFGT HSVEATIDNG DFGSRTIRLE
60 70 80 90 100
TGQLARQANG SVVAYLDEDT MMLSTTTASS KPREGFDFFP LTVDVEERMY
110 120 130 140 150
AAGRIPGSFF RREGRPGTDA ILACRLIDRP LRPTFVKGLR NEVQVIVTVM
160 170 180 190 200
SLDPKDMYDV VAINAASAST QLSGLPVSGP VGGVRMALIV DDDHEDGQWV
210 220 230 240 250
AFPTREQHES ALFEMVVAGR LTDEQVPEKP RKGRRRGRKS SPRKKTDNVA
260 270 280 290 300
IMMVEAGATE TVVERVNDGA PAPTESVVAE GIEAAKPFIA ELCGAQQALR
310 320 330 340 350
QAVDPETEEF PLFPPYGADV LAAVDSEAKG RISDIMSIAD KQERDEALSS
360 370 380 390 400
DMDDTVEALL EEFPEREAEI RAAHNAVTKE VVRSRILADG FRIDGRGVED
410 420 430 440 450
IRDLDVEVDL VPRAHGSSLF QRGETQILGV TTLDTLKMEQ QVDSLGPVDH
460 470 480 490 500
RRYIHHYNFP PYSTGETGRV GSPKRREIGH GALAERALKP MIPSRDDFPY
510 520 530 540 550
TIRQVSEALG SNGSTSMGSV CASTLSLYNA GVPLKAPVAG IAMGLVSGEV
560 570 580 590 600
KGKMTYVTLT DILGAEDAFG DMDFKVAGTE DFITALQLDT KLDGIPSDVL
610 620 630 640 650
AGALKQAREA RLEILNTMAE VIDEPDPMSD YAPRITTISV PVSKIGEVIG
660 670 680 690 700
PKGKNINQIT EDTGARVSIE DDGTVFISAT SGGSAEAAVD RINEIANPQL
710 720 730 740 750
PKVGERFLGT VVKTTAFGAF VSILPNRDGL VHISKLGGKK RIEKVEDVVN
760 770
VGDKLEVEIA DIDNRGKISL VPVDD
Length:775
Mass (Da):83,942
Last modified:July 7, 2009 - v1
Checksum:i46D34369E29AF770
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001620 Genomic DNA. Translation: ACR17879.1.
RefSeqiWP_012731766.1. NC_012704.1.
YP_002906422.1. NC_012704.1.

Genome annotation databases

EnsemblBacteriaiACR17879; ACR17879; ckrop_1133.
GeneIDi7876072.
KEGGickp:ckrop_1133.
PATRICi21517605. VBICorKro120627_1167.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001620 Genomic DNA. Translation: ACR17879.1.
RefSeqiWP_012731766.1. NC_012704.1.
YP_002906422.1. NC_012704.1.

3D structure databases

ProteinModelPortaliC4LJ74.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi645127.ckrop_1133.

Proteomic databases

PRIDEiC4LJ74.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACR17879; ACR17879; ckrop_1133.
GeneIDi7876072.
KEGGickp:ckrop_1133.
PATRICi21517605. VBICorKro120627_1167.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciCKRO645127:GI7D-1123-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Ultrafast pyrosequencing of Corynebacterium kroppenstedtii DSM44385 revealed insights into the physiology of a lipophilic corynebacterium that lacks mycolic acids."
    Tauch A., Schneider J., Szczepanowski R., Tilker A., Viehoever P., Gartemann K.-H., Arnold W., Blom J., Brinkrolf K., Brune I., Goetker S., Weisshaar B., Goesmann A., Droege M., Puehler A.
    J. Biotechnol. 136:22-30(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 44385 / CCUG 35717.

Entry informationi

Entry nameiPNP_CORK4
AccessioniPrimary (citable) accession number: C4LJ74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: July 7, 2009
Last modified: March 4, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.