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C4LJ74

- PNP_CORK4

UniProt

C4LJ74 - PNP_CORK4

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Protein

Polyribonucleotide nucleotidyltransferase

Gene
pnp, ckrop_1133
Organism
Corynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi567 – 5671Magnesium By similarity
Metal bindingi573 – 5731Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciCKRO645127:GI7D-1123-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:ckrop_1133
OrganismiCorynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717)
Taxonomic identifieri645127 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000001473: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 775775Polyribonucleotide nucleotidyltransferaseUniRule annotationPRO_0000382421Add
BLAST

Proteomic databases

PRIDEiC4LJ74.

Interactioni

Protein-protein interaction databases

STRINGi645127.ckrop_1133.

Structurei

3D structure databases

ProteinModelPortaliC4LJ74.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini633 – 69260KHAdd
BLAST
Domaini704 – 77370S1 motifAdd
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiTIAGKQD.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C4LJ74-1 [UniParc]FASTAAdd to Basket

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MIARGTHREG RIPPFMTNNH SVFYDEEFGT HSVEATIDNG DFGSRTIRLE    50
TGQLARQANG SVVAYLDEDT MMLSTTTASS KPREGFDFFP LTVDVEERMY 100
AAGRIPGSFF RREGRPGTDA ILACRLIDRP LRPTFVKGLR NEVQVIVTVM 150
SLDPKDMYDV VAINAASAST QLSGLPVSGP VGGVRMALIV DDDHEDGQWV 200
AFPTREQHES ALFEMVVAGR LTDEQVPEKP RKGRRRGRKS SPRKKTDNVA 250
IMMVEAGATE TVVERVNDGA PAPTESVVAE GIEAAKPFIA ELCGAQQALR 300
QAVDPETEEF PLFPPYGADV LAAVDSEAKG RISDIMSIAD KQERDEALSS 350
DMDDTVEALL EEFPEREAEI RAAHNAVTKE VVRSRILADG FRIDGRGVED 400
IRDLDVEVDL VPRAHGSSLF QRGETQILGV TTLDTLKMEQ QVDSLGPVDH 450
RRYIHHYNFP PYSTGETGRV GSPKRREIGH GALAERALKP MIPSRDDFPY 500
TIRQVSEALG SNGSTSMGSV CASTLSLYNA GVPLKAPVAG IAMGLVSGEV 550
KGKMTYVTLT DILGAEDAFG DMDFKVAGTE DFITALQLDT KLDGIPSDVL 600
AGALKQAREA RLEILNTMAE VIDEPDPMSD YAPRITTISV PVSKIGEVIG 650
PKGKNINQIT EDTGARVSIE DDGTVFISAT SGGSAEAAVD RINEIANPQL 700
PKVGERFLGT VVKTTAFGAF VSILPNRDGL VHISKLGGKK RIEKVEDVVN 750
VGDKLEVEIA DIDNRGKISL VPVDD 775
Length:775
Mass (Da):83,942
Last modified:July 7, 2009 - v1
Checksum:i46D34369E29AF770
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001620 Genomic DNA. Translation: ACR17879.1.
RefSeqiWP_012731766.1. NC_012704.1.
YP_002906422.1. NC_012704.1.

Genome annotation databases

EnsemblBacteriaiACR17879; ACR17879; ckrop_1133.
GeneIDi7876072.
KEGGickp:ckrop_1133.
PATRICi21517605. VBICorKro120627_1167.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001620 Genomic DNA. Translation: ACR17879.1 .
RefSeqi WP_012731766.1. NC_012704.1.
YP_002906422.1. NC_012704.1.

3D structure databases

ProteinModelPortali C4LJ74.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 645127.ckrop_1133.

Proteomic databases

PRIDEi C4LJ74.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACR17879 ; ACR17879 ; ckrop_1133 .
GeneIDi 7876072.
KEGGi ckp:ckrop_1133.
PATRICi 21517605. VBICorKro120627_1167.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi TIAGKQD.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci CKRO645127:GI7D-1123-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 1 hit.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Ultrafast pyrosequencing of Corynebacterium kroppenstedtii DSM44385 revealed insights into the physiology of a lipophilic corynebacterium that lacks mycolic acids."
    Tauch A., Schneider J., Szczepanowski R., Tilker A., Viehoever P., Gartemann K.-H., Arnold W., Blom J., Brinkrolf K., Brune I., Goetker S., Weisshaar B., Goesmann A., Droege M., Puehler A.
    J. Biotechnol. 136:22-30(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 44385 / CCUG 35717.

Entry informationi

Entry nameiPNP_CORK4
AccessioniPrimary (citable) accession number: C4LJ74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: July 7, 2009
Last modified: September 3, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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