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C4LJ74

- PNP_CORK4

UniProt

C4LJ74 - PNP_CORK4

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Corynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (07 Jul 2009)
      Previous versions | rss
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    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi567 – 5671MagnesiumUniRule annotation
    Metal bindingi573 – 5731MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciCKRO645127:GI7D-1123-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:ckrop_1133
    OrganismiCorynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717)
    Taxonomic identifieri645127 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000001473: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 775775Polyribonucleotide nucleotidyltransferasePRO_0000382421Add
    BLAST

    Proteomic databases

    PRIDEiC4LJ74.

    Interactioni

    Protein-protein interaction databases

    STRINGi645127.ckrop_1133.

    Structurei

    3D structure databases

    ProteinModelPortaliC4LJ74.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini633 – 69260KHUniRule annotationAdd
    BLAST
    Domaini704 – 77370S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    HOGENOMiHOG000218326.
    KOiK00962.
    OMAiTIAGKQD.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR014069. pppGpp_PNP.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 1 hit.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    TIGR02696. pppGpp_PNP. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C4LJ74-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIARGTHREG RIPPFMTNNH SVFYDEEFGT HSVEATIDNG DFGSRTIRLE    50
    TGQLARQANG SVVAYLDEDT MMLSTTTASS KPREGFDFFP LTVDVEERMY 100
    AAGRIPGSFF RREGRPGTDA ILACRLIDRP LRPTFVKGLR NEVQVIVTVM 150
    SLDPKDMYDV VAINAASAST QLSGLPVSGP VGGVRMALIV DDDHEDGQWV 200
    AFPTREQHES ALFEMVVAGR LTDEQVPEKP RKGRRRGRKS SPRKKTDNVA 250
    IMMVEAGATE TVVERVNDGA PAPTESVVAE GIEAAKPFIA ELCGAQQALR 300
    QAVDPETEEF PLFPPYGADV LAAVDSEAKG RISDIMSIAD KQERDEALSS 350
    DMDDTVEALL EEFPEREAEI RAAHNAVTKE VVRSRILADG FRIDGRGVED 400
    IRDLDVEVDL VPRAHGSSLF QRGETQILGV TTLDTLKMEQ QVDSLGPVDH 450
    RRYIHHYNFP PYSTGETGRV GSPKRREIGH GALAERALKP MIPSRDDFPY 500
    TIRQVSEALG SNGSTSMGSV CASTLSLYNA GVPLKAPVAG IAMGLVSGEV 550
    KGKMTYVTLT DILGAEDAFG DMDFKVAGTE DFITALQLDT KLDGIPSDVL 600
    AGALKQAREA RLEILNTMAE VIDEPDPMSD YAPRITTISV PVSKIGEVIG 650
    PKGKNINQIT EDTGARVSIE DDGTVFISAT SGGSAEAAVD RINEIANPQL 700
    PKVGERFLGT VVKTTAFGAF VSILPNRDGL VHISKLGGKK RIEKVEDVVN 750
    VGDKLEVEIA DIDNRGKISL VPVDD 775
    Length:775
    Mass (Da):83,942
    Last modified:July 7, 2009 - v1
    Checksum:i46D34369E29AF770
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001620 Genomic DNA. Translation: ACR17879.1.
    RefSeqiWP_012731766.1. NC_012704.1.
    YP_002906422.1. NC_012704.1.

    Genome annotation databases

    EnsemblBacteriaiACR17879; ACR17879; ckrop_1133.
    GeneIDi7876072.
    KEGGickp:ckrop_1133.
    PATRICi21517605. VBICorKro120627_1167.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001620 Genomic DNA. Translation: ACR17879.1 .
    RefSeqi WP_012731766.1. NC_012704.1.
    YP_002906422.1. NC_012704.1.

    3D structure databases

    ProteinModelPortali C4LJ74.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 645127.ckrop_1133.

    Proteomic databases

    PRIDEi C4LJ74.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACR17879 ; ACR17879 ; ckrop_1133 .
    GeneIDi 7876072.
    KEGGi ckp:ckrop_1133.
    PATRICi 21517605. VBICorKro120627_1167.

    Phylogenomic databases

    eggNOGi COG1185.
    HOGENOMi HOG000218326.
    KOi K00962.
    OMAi TIAGKQD.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci CKRO645127:GI7D-1123-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR014069. pppGpp_PNP.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 1 hit.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    TIGR02696. pppGpp_PNP. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Ultrafast pyrosequencing of Corynebacterium kroppenstedtii DSM44385 revealed insights into the physiology of a lipophilic corynebacterium that lacks mycolic acids."
      Tauch A., Schneider J., Szczepanowski R., Tilker A., Viehoever P., Gartemann K.-H., Arnold W., Blom J., Brinkrolf K., Brune I., Goetker S., Weisshaar B., Goesmann A., Droege M., Puehler A.
      J. Biotechnol. 136:22-30(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 44385 / CCUG 35717.

    Entry informationi

    Entry nameiPNP_CORK4
    AccessioniPrimary (citable) accession number: C4LJ74
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 1, 2009
    Last sequence update: July 7, 2009
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3