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Protein

L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

Gene

mshC

Organism
Corynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins.UniRule annotation

Catalytic activityi

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-(2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + AMP + diphosphate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481ZincUniRule annotation
Binding sitei63 – 631Cysteinyl adenylateUniRule annotation
Binding sitei232 – 2321Cysteinyl adenylateUniRule annotation
Metal bindingi236 – 2361ZincUniRule annotation
Metal bindingi261 – 2611ZincUniRule annotation
Binding sitei288 – 2881Cysteinyl adenylate; via amide nitrogen and carbonyl oxygenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCKRO645127:GI7D-910-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseUniRule annotation (EC:6.3.1.13UniRule annotation)
Short name:
L-Cys:GlcN-Ins ligaseUniRule annotation
Alternative name(s):
Mycothiol ligaseUniRule annotation
Short name:
MSH ligaseUniRule annotation
Gene namesi
Name:mshCUniRule annotation
Ordered Locus Names:ckrop_0920
OrganismiCorynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717)
Taxonomic identifieri645127 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000001473 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 520520L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligasePRO_0000400441Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi645127.ckrop_0920.

Structurei

3D structure databases

ProteinModelPortaliC4LIL9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni48 – 514Cysteinyl adenylate bindingUniRule annotation
Regioni86 – 883Cysteinyl adenylate bindingUniRule annotation
Regioni254 – 2563Cysteinyl adenylate bindingUniRule annotation

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi50 – 6011"HIGH" regionUniRule annotationAdd
BLAST
Motifi192 – 1976"ERGGDP" regionUniRule annotation
Motifi294 – 2985"KMSKS" regionUniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiARHYVHT.
OrthoDBiEOG6RVFXC.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.

Sequencei

Sequence statusi: Complete.

C4LIL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSWQKPLVA DFRSVVGEDQ PVPLHMWDTA SREVTPLTPS DEATVYVCGI
60 70 80 90 100
TPYDSTHLGH AATYVTFDVL NRQLLANGHR VNFVQNVTDV DDPLFERAQR
110 120 130 140 150
DGVDWQELGE EQTDLFRQDM ANLGVIPPTS YIGAVEAIPD VIDMVSTLLD
160 170 180 190 200
NGSAYVVDDP TYPDVYADRT ATPQFGYESR YDEATMEQFF AERGGDPDRP
210 220 230 240 250
GKRDPLDSLL WRAERPGEPA WDSPFGRGRP GWHIECAAIA ARYLGSHFDV
260 270 280 290 300
QGGGEDLIFP HHEYSAAHVE AACGVERMAD HYMHTGLIAL GGVKMSKSLG
310 320 330 340 350
NLVFVSRLCN AGTDPSSIRL ALYAGHYRDQ RNWSDALLAA ASRRLALWRT
360 370 380 390 400
AVETVHAHND ADSEARARRL VDDVRQALAT DLDTPAAVDL VDEWAGSIVD
410 420 430 440 450
KEKIWDLPGV DGKNAHESLS VPVPCAAPAT DDKLSVAQGK ITILDDGKDS
460 470 480 490 500
EADAKSVELA SATAGTQRCG AHSHASGELT VDLLPAPHQR GDAPSALYSH
510 520
LSPAAALVAG CVDALLGLKL
Length:520
Mass (Da):56,394
Last modified:July 7, 2009 - v1
Checksum:iF59AF8023549161E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001620 Genomic DNA. Translation: ACR17674.1.

Genome annotation databases

EnsemblBacteriaiACR17674; ACR17674; ckrop_0920.
KEGGickp:ckrop_0920.
PATRICi21517155. VBICorKro120627_0948.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001620 Genomic DNA. Translation: ACR17674.1.

3D structure databases

ProteinModelPortaliC4LIL9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi645127.ckrop_0920.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACR17674; ACR17674; ckrop_0920.
KEGGickp:ckrop_0920.
PATRICi21517155. VBICorKro120627_0948.

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiARHYVHT.
OrthoDBiEOG6RVFXC.

Enzyme and pathway databases

BioCyciCKRO645127:GI7D-910-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Ultrafast pyrosequencing of Corynebacterium kroppenstedtii DSM44385 revealed insights into the physiology of a lipophilic corynebacterium that lacks mycolic acids."
    Tauch A., Schneider J., Szczepanowski R., Tilker A., Viehoever P., Gartemann K.-H., Arnold W., Blom J., Brinkrolf K., Brune I., Goetker S., Weisshaar B., Goesmann A., Droege M., Puehler A.
    J. Biotechnol. 136:22-30(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 44385 / CCUG 35717.

Entry informationi

Entry nameiMSHC_CORK4
AccessioniPrimary (citable) accession number: C4LIL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: July 7, 2009
Last modified: January 20, 2016
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.