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C4LI04

- LIPA_CORK4

UniProt

C4LI04 - LIPA_CORK4

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Protein
Lipoyl synthase
Gene
lipA, ckrop_0698
Organism
Corynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi55 – 551Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi60 – 601Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi81 – 811Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi85 – 851Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi88 – 881Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCKRO645127:GI7D-690-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:ckrop_0698
OrganismiCorynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717)
Taxonomic identifieri645127 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000001473: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 347347Lipoyl synthaseUniRule annotation
PRO_1000204145Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi645127.ckrop_0698.

Structurei

3D structure databases

ProteinModelPortaliC4LI04.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiEWLRRPI.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

C4LI04-1 [UniParc]FASTAAdd to Basket

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MAVAPNGRRL LRIEARNAQT PIEAKPRWIR TTATMGPEFR DMKKRVKGAG    50
LHTVCQEAGC PNIHECWEDR EATFLIGGDT CSRRCDFCDI KSGRPEPLDE 100
DEPRRVAENV REIGLRYSTI TGVTRDDLPD EGAWLYAEVV RQIHKLNPNT 150
GVENLTPDFS GKPDLLQTVF EARPEVFAHN LETVPRIFKR IRPAFRYERS 200
LDVIRQARDF GLVTKSNLIL GMGETVDEVK SAMRDLRDAG CDILTVTQYL 250
RPSNLHHPIE RWVKPEEFVM YRDYGHDIGF AGVMAGPLVR SSYRAGRLYA 300
QAIKARGEEL PANLQHLGEG IDDTASQEAS TLLSKYGASR ETPVGAR 347
Length:347
Mass (Da):39,087
Last modified:July 7, 2009 - v1
Checksum:i5AAE1555D236514D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001620 Genomic DNA. Translation: ACR17459.1.
RefSeqiYP_002906002.1. NC_012704.1.

Genome annotation databases

EnsemblBacteriaiACR17459; ACR17459; ckrop_0698.
GeneIDi7876692.
KEGGickp:ckrop_0698.
PATRICi21516695. VBICorKro120627_0723.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001620 Genomic DNA. Translation: ACR17459.1 .
RefSeqi YP_002906002.1. NC_012704.1.

3D structure databases

ProteinModelPortali C4LI04.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 645127.ckrop_0698.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACR17459 ; ACR17459 ; ckrop_0698 .
GeneIDi 7876692.
KEGGi ckp:ckrop_0698.
PATRICi 21516695. VBICorKro120627_0723.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi EWLRRPI.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci CKRO645127:GI7D-690-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Ultrafast pyrosequencing of Corynebacterium kroppenstedtii DSM44385 revealed insights into the physiology of a lipophilic corynebacterium that lacks mycolic acids."
    Tauch A., Schneider J., Szczepanowski R., Tilker A., Viehoever P., Gartemann K.-H., Arnold W., Blom J., Brinkrolf K., Brune I., Goetker S., Weisshaar B., Goesmann A., Droege M., Puehler A.
    J. Biotechnol. 136:22-30(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 44385 / CCUG 35717.

Entry informationi

Entry nameiLIPA_CORK4
AccessioniPrimary (citable) accession number: C4LI04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 7, 2009
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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