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C4LGT5

- PCKG_CORK4

UniProt

C4LGT5 - PCKG_CORK4

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Protein
Phosphoenolpyruvate carboxykinase [GTP]
Gene
pckG, ckrop_0253
Organism
Corynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei82 – 821Substrate By similarity
Binding sitei222 – 2221Substrate; via amide nitrogen By similarity
Metal bindingi229 – 2291Manganese By similarity
Binding sitei229 – 2291Substrate By similarity
Metal bindingi249 – 2491Manganese; via tele nitrogen By similarity
Binding sitei271 – 2711Substrate By similarity
Active sitei273 – 2731 By similarity
Metal bindingi296 – 2961Manganese By similarity
Binding sitei388 – 3881GTP By similarity
Binding sitei419 – 4191GTP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi514 – 5174GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCKRO645127:GI7D-254-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEP carboxykinase
Short name:
PEPCK
Gene namesi
Name:pckG
Ordered Locus Names:ckrop_0253
OrganismiCorynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717)
Taxonomic identifieri645127 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000001473: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 621621Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation
PRO_1000206232Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi645127.ckrop_0253.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni386 – 3883Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiGNNIFTN.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.

Sequencei

Sequence statusi: Complete.

C4LGT5-1 [UniParc]FASTAAdd to Basket

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MSAPTIPGLE GNAPTTNQDM LNWIAECAEL CQPDKVVFCD GSDEEWEAIA    50
KDLVEKGTLI KLNEEKRPNS YLASSDPADV ARVESRTFIC SKTEDGAGPT 100
NNWRDPDEMR AEMSEHFKGS MKGRTMYVVP FCMGPITDPD PKLGIELTDS 150
GYVVMSMRIM TRMGKEALDK IGDGPFVKGL HSVGAPLEPG QEDVKWPCNE 200
TKYITQFPED RVIWSYGSGY GGNAILAKKC YALRIASVMA KDEGWMAEHM 250
LILKLISPEG KAYHICAAFP SQCGKTNLAM IQPTIPGWKA EVIGDDIAWL 300
HFGDDGRLYA VNPENGFFGV APGTNYKSNP NAMKTMEAGN NIFTNVALTD 350
DGDVWWEGLE NEPDHLIDWK GKDWTPDEDC LSSHPNSRYC VPIEQCPVAA 400
PEFNDPKGVP VDAILFGGRR PDTVPLVTEA IDWQHATFIG ATLASGQTAA 450
AAEAKVGTLR YDPMAMLPFI GYNVGDYLQH WLDIGKKGGD KLPKVFLVNW 500
FRRGDDGRFL WPGFGENSRV LKWIVDRIEG RVDAKKTVVG YTAYSKDIDI 550
DGLDTDPKDV KAALTAPADK WQMDLSDTED WLKSLGPKVP QEIWDEFDFL 600
KTRIKTANKD GNKALDNMKT K 621
Length:621
Mass (Da):68,814
Last modified:July 7, 2009 - v1
Checksum:iD70178D80F67B5FE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001620 Genomic DNA. Translation: ACR17040.1.
RefSeqiYP_002905583.1. NC_012704.1.

Genome annotation databases

EnsemblBacteriaiACR17040; ACR17040; ckrop_0253.
GeneIDi7875443.
KEGGickp:ckrop_0253.
PATRICi21515743. VBICorKro120627_0263.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001620 Genomic DNA. Translation: ACR17040.1 .
RefSeqi YP_002905583.1. NC_012704.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 645127.ckrop_0253.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACR17040 ; ACR17040 ; ckrop_0253 .
GeneIDi 7875443.
KEGGi ckp:ckrop_0253.
PATRICi 21515743. VBICorKro120627_0263.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi GNNIFTN.
OrthoDBi EOG6MPWQS.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci CKRO645127:GI7D-254-MONOMER.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Ultrafast pyrosequencing of Corynebacterium kroppenstedtii DSM44385 revealed insights into the physiology of a lipophilic corynebacterium that lacks mycolic acids."
    Tauch A., Schneider J., Szczepanowski R., Tilker A., Viehoever P., Gartemann K.-H., Arnold W., Blom J., Brinkrolf K., Brune I., Goetker S., Weisshaar B., Goesmann A., Droege M., Puehler A.
    J. Biotechnol. 136:22-30(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 44385 / CCUG 35717.

Entry informationi

Entry nameiPCKG_CORK4
AccessioniPrimary (citable) accession number: C4LGT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 7, 2009
Last modified: May 14, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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