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C4LGT5

- PCKG_CORK4

UniProt

C4LGT5 - PCKG_CORK4

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Corynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (07 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei82 – 821SubstrateUniRule annotation
    Binding sitei222 – 2221Substrate; via amide nitrogenUniRule annotation
    Metal bindingi229 – 2291ManganeseUniRule annotation
    Binding sitei229 – 2291SubstrateUniRule annotation
    Metal bindingi249 – 2491Manganese; via tele nitrogenUniRule annotation
    Binding sitei271 – 2711SubstrateUniRule annotation
    Active sitei273 – 2731UniRule annotation
    Metal bindingi296 – 2961ManganeseUniRule annotation
    Binding sitei388 – 3881GTPUniRule annotation
    Binding sitei419 – 4191GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi514 – 5174GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCKRO645127:GI7D-254-MONOMER.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Ordered Locus Names:ckrop_0253
    OrganismiCorynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717)
    Taxonomic identifieri645127 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000001473: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 621621Phosphoenolpyruvate carboxykinase [GTP]PRO_1000206232Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi645127.ckrop_0253.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni386 – 3883Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    KOiK01596.
    OMAiGNNIFTN.
    OrthoDBiEOG6MPWQS.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    C4LGT5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAPTIPGLE GNAPTTNQDM LNWIAECAEL CQPDKVVFCD GSDEEWEAIA    50
    KDLVEKGTLI KLNEEKRPNS YLASSDPADV ARVESRTFIC SKTEDGAGPT 100
    NNWRDPDEMR AEMSEHFKGS MKGRTMYVVP FCMGPITDPD PKLGIELTDS 150
    GYVVMSMRIM TRMGKEALDK IGDGPFVKGL HSVGAPLEPG QEDVKWPCNE 200
    TKYITQFPED RVIWSYGSGY GGNAILAKKC YALRIASVMA KDEGWMAEHM 250
    LILKLISPEG KAYHICAAFP SQCGKTNLAM IQPTIPGWKA EVIGDDIAWL 300
    HFGDDGRLYA VNPENGFFGV APGTNYKSNP NAMKTMEAGN NIFTNVALTD 350
    DGDVWWEGLE NEPDHLIDWK GKDWTPDEDC LSSHPNSRYC VPIEQCPVAA 400
    PEFNDPKGVP VDAILFGGRR PDTVPLVTEA IDWQHATFIG ATLASGQTAA 450
    AAEAKVGTLR YDPMAMLPFI GYNVGDYLQH WLDIGKKGGD KLPKVFLVNW 500
    FRRGDDGRFL WPGFGENSRV LKWIVDRIEG RVDAKKTVVG YTAYSKDIDI 550
    DGLDTDPKDV KAALTAPADK WQMDLSDTED WLKSLGPKVP QEIWDEFDFL 600
    KTRIKTANKD GNKALDNMKT K 621
    Length:621
    Mass (Da):68,814
    Last modified:July 7, 2009 - v1
    Checksum:iD70178D80F67B5FE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001620 Genomic DNA. Translation: ACR17040.1.
    RefSeqiYP_002905583.1. NC_012704.1.

    Genome annotation databases

    EnsemblBacteriaiACR17040; ACR17040; ckrop_0253.
    GeneIDi7875443.
    KEGGickp:ckrop_0253.
    PATRICi21515743. VBICorKro120627_0263.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001620 Genomic DNA. Translation: ACR17040.1 .
    RefSeqi YP_002905583.1. NC_012704.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 645127.ckrop_0253.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACR17040 ; ACR17040 ; ckrop_0253 .
    GeneIDi 7875443.
    KEGGi ckp:ckrop_0253.
    PATRICi 21515743. VBICorKro120627_0263.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    KOi K01596.
    OMAi GNNIFTN.
    OrthoDBi EOG6MPWQS.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BioCyci CKRO645127:GI7D-254-MONOMER.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Ultrafast pyrosequencing of Corynebacterium kroppenstedtii DSM44385 revealed insights into the physiology of a lipophilic corynebacterium that lacks mycolic acids."
      Tauch A., Schneider J., Szczepanowski R., Tilker A., Viehoever P., Gartemann K.-H., Arnold W., Blom J., Brinkrolf K., Brune I., Goetker S., Weisshaar B., Goesmann A., Droege M., Puehler A.
      J. Biotechnol. 136:22-30(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 44385 / CCUG 35717.

    Entry informationi

    Entry nameiPCKG_CORK4
    AccessioniPrimary (citable) accession number: C4LGT5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: July 7, 2009
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3