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C4LEY6 (NAGZ_TOLAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-hexosaminidase

EC=3.2.1.52
Alternative name(s):
Beta-N-acetylhexosaminidase
N-acetyl-beta-glucosaminidase
Gene names
Name:nagZ
Ordered Locus Names:Tola_1542
OrganismTolumonas auensis (strain DSM 9187 / TA4) [Complete proteome] [HAMAP]
Taxonomic identifier595494 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeTolumonas

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides By similarity. HAMAP-Rule MF_00364

Catalytic activity

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP-Rule MF_00364

Pathway

Cell wall biogenesis; peptidoglycan recycling. HAMAP-Rule MF_00364

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00364.

Sequence similarities

Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334Beta-hexosaminidase HAMAP-Rule MF_00364
PRO_1000205462

Regions

Region161 – 1622Substrate binding By similarity

Sites

Active site1741Proton donor/acceptor By similarity
Active site2461Nucleophile By similarity
Binding site621Substrate By similarity
Binding site701Substrate By similarity
Binding site1311Substrate By similarity
Site1721Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
C4LEY6 [UniParc].

Last modified July 7, 2009. Version 1.
Checksum: 5C9336D8B5FF358F

FASTA33436,672
        10         20         30         40         50         60 
MGPLMLDVQG CELDAEEREI LRHPTVGGVI LFGRNYHDRS QLSALVKTIR QAANKPLLIA 

        70         80         90        100        110        120 
VDHEGGRVQR FRNGFSRIPP MGILHKARNQ ATDLATECGW LMAAELLAHD IDLSFAPVLD 

       130        140        150        160        170        180 
LERGSNVIGD RSFSSDPEQV IQLASAFIDG MHQAGMKATG KHFPGHGSVR ADSHLESPRD 

       190        200        210        220        230        240 
NRSWEEIEAT DLVPFRELIP AGKLDAIMPA HVIYTELDDQ PAGFSRFWLQ EVLRGKYGFN 

       250        260        270        280        290        300 
GIIFSDDLTM EGAAVAGGYP ERAQAALNAG CDMLLACNNR AGAVAILDGL QNPPASRAES 

       310        320        330 
LLSTNNGDWS RLTSTSRWKN TQNRVQIFTE EFVN 

« Hide

References

[1]"Complete sequence of Tolumonas auensis DSM 9187."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Spring S., Beller H.
Submitted (MAY-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 9187 / TA4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001616 Genomic DNA. Translation: ACQ93153.1.
RefSeqYP_002892739.1. NC_012691.1.

3D structure databases

ProteinModelPortalC4LEY6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING595494.Tola_1542.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACQ93153; ACQ93153; Tola_1542.
GeneID7885143.
KEGGtau:Tola_1542.
PATRIC23979715. VBITolAue42623_1534.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1472.
HOGENOMHOG000248526.
KOK01207.
OMAAHDIDLS.
OrthoDBEOG6BCT06.

Enzyme and pathway databases

BioCycTAUE595494:GHEF-1593-MONOMER.
UniPathwayUPA00544.

Family and domain databases

Gene3D3.20.20.300. 1 hit.
HAMAPMF_00364. NagZ.
InterProIPR022956. Beta_hexosaminidase_bac.
IPR019800. Glyco_hydro_3_AS.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAGZ_TOLAT
AccessionPrimary (citable) accession number: C4LEY6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 7, 2009
Last modified: May 14, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries