C4LCB2 (C4LCB2_TOLAT) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Leucine--tRNA ligase HAMAP-Rule MF_00049
EC=6.1.1.4 HAMAP-Rule MF_00049

Alternative name(s):
Leucyl-tRNA synthetase HAMAP-Rule MF_00049

Gene names

Name:	leuS HAMAP-Rule MF_00049
Ordered Locus Names:	Tola_2823 EMBL ACQ94416.1

Organism

Tolumonas auensis (strain DSM 9187 / TA4) [Complete proteome] [HAMAP] EMBL ACQ94416.1

Taxonomic identifier

595494 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Tolumonas › Tolumonas auensis

Protein attributes

Sequence length	861 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00049.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP-Rule MF_00049 RuleBase RU003489

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00049 RuleBase RU003489
Cellular component	Cytoplasm HAMAP-Rule MF_00049
Ligand	ATP-binding HAMAP-Rule MF_00049 RuleBase RU003489 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP-Rule MF_00049 RuleBase RU003489 EMBL ACQ94416.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro leucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Motif	45 – 55	11	"HIGH" region By similarity HAMAP-Rule MF_00049
	Motif	621 – 625	5	"KMSKS" region By similarity HAMAP-Rule MF_00049
Sites
	Binding site	624	1	ATP By similarity HAMAP-Rule MF_00049

Sequences

	Sequence		Length	Mass (Da)
	C4LCB2 [UniParc]. Last modified July 7, 2009. Version 1. Checksum: 7182C3BC2D0B2E61	FASTA	861	97,335
	10 20 30 40 50 60 MSVMQEQYNP QSLEPEVQRH WDKQQTFKAF EKVDKEKFYC LSMFPYPSGR LHMGHVRNYT 70 80 90 100 110 120 IGDVISRYQR LNGKNVLQPI GWDAFGLPAE NAAIKNNSAP AKWTYENIEY MKGQLKMLGL 130 140 150 160 170 180 SYDWDRELAT CTPQYYRWEQ WFFTELYKKG LVYKKTSSVN WCPNDQTVLA NEQVQDGCCW 190 200 210 220 230 240 RCDTPVEQKE IPQWFVKITA YAEELLTDLD KLDGWPEMVK TMQRNWIGRS EGLTMTFHVE 250 260 270 280 290 300 NSEQSFDIYT TRPDTLMGVT YVAVAAAHPL AKQAAENNAA LADFLEECKN TKVAEAELAT 310 320 330 340 350 360 MEKKGMATGL YALHPLDGRR VPIMVANFVL MDYGTGAVMA VPAHDQRDFE FAHKYGLEIR 370 380 390 400 410 420 AVIAAEDGSA PDISAAAYTE KGVLFNSGEF DGLDFKQAFD AICTKLESQG LGKRTVNFRL 430 440 450 460 470 480 RDWGVSRQRY WGAPIPMLTL EDGTVVPAPA DQLPVILPED VVMDGVQSPI KADPEWAKTT 490 500 510 520 530 540 YNGQPALRET DTFDTFMESS WYFARYCCPD YEQGMLDTDR ANHWLPVDQY IGGIEHACMH 550 560 570 580 590 600 LLYARFFHKL LRDSGMVKSD EPFTRLLCQG MVLADAFYYT EHGARVWVSP TDVKVERDEK 610 620 630 640 650 660 GRIIKAVDNE GREVIHAGMT KMSKSKNNGI DPQLMVERYG ADTVRLFMMF ASPADMTLEW 670 680 690 700 710 720 QESGVDGAQR FLKRLWKAVA EHTAKGAAPE LDVASLSNEQ KTLRRELHKT IAKFSDDIGR 730 740 750 760 770 780 RQTFNTAIAA VMELMNRVAK APQESDQDRA LVQEVLTNVV LMLYPITPHV CFHLWQALGN 790 800 810 820 830 840 ADIDQAAWPV ADAAAMVEDE KLVVVQVNGK VRGKLTVAAD ATQEQVQALA MQDHTVAKFT 850 860 ADLTVRKVIY VPGKLLNIVV G

References

[1]

"Complete sequence of Tolumonas auensis DSM 9187." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Spring S., Beller H. Submitted (MAY-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 9187 / TA4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001616 Genomic DNA. Translation: ACQ94416.1.
RefSeq	YP_002894002.1. NC_012691.1.
3D structure databases
ProteinModelPortal	C4LCB2.
ModBase	Search...
Protein-protein interaction databases
STRING	595494.Tola_2823.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACQ94416; ACQ94416; Tola_2823.
GeneID	7885659.
KEGG	tau:Tola_2823.
PATRIC	23982383. VBITolAue42623_2810.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0495.
HOGENOM	HOG000200747.
KO	K01869.
ProtClustDB	PRK00390.
Enzyme and pathway databases
BioCyc	TAUE595494:GHEF-2934-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 3 hits. 3.90.740.10. 1 hit.
HAMAP	MF_00049_B. Leu_tRNA_synth_B.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002302. Leu-tRNA-ligase_bac/mito. IPR025709. Leu_tRNA-synth_edit. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
PANTHER	PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 3 hits. PF13603. tRNA-synt_1_2. 1 hit. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00396. leuS_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C4LCB2_TOLAT

Accession

Primary (citable) accession number: C4LCB2

Entry history

Integrated into UniProtKB/TrEMBL:	July 7, 2009
Last sequence update:	July 7, 2009
Last modified:	May 29, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)