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Protein

Siroheme synthase

Gene

cysG

Organism
Tolumonas auensis (strain DSM 9187 / TA4)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.UniRule annotation
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.UniRule annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei223 – 2231S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei246 – 2461Proton acceptorUniRule annotation
Active sitei268 – 2681Proton donorUniRule annotation
Binding sitei381 – 3811S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei410 – 4101S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 232NADUniRule annotation
Nucleotide bindingi43 – 442NADUniRule annotation

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. precorrin-2 dehydrogenase activity Source: UniProtKB-HAMAP
  3. sirohydrochlorin ferrochelatase activity Source: UniProtKB-EC
  4. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-HAMAP
  2. siroheme biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NAD, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciTAUE595494:GHEF-2627-MONOMER.
UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthaseUniRule annotation
Including the following 3 domains:
Uroporphyrinogen-III C-methyltransferaseUniRule annotation (EC:2.1.1.107UniRule annotation)
Short name:
Urogen III methylaseUniRule annotation
Alternative name(s):
SUMTUniRule annotation
Uroporphyrinogen III methylaseUniRule annotation
Short name:
UROMUniRule annotation
Precorrin-2 dehydrogenaseUniRule annotation (EC:1.3.1.76UniRule annotation)
Sirohydrochlorin ferrochelataseUniRule annotation (EC:4.99.1.4UniRule annotation)
Gene namesi
Name:cysGUniRule annotation
Ordered Locus Names:Tola_2546
OrganismiTolumonas auensis (strain DSM 9187 / TA4)
Taxonomic identifieri595494 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeTolumonas
ProteomesiUP000009073: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 468468Siroheme synthasePRO_1000215848Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei126 – 1261PhosphoserineUniRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi595494.Tola_2546.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 202202precorrin-2 dehydrogenase / sirohydrochlorin ferrochelataseUniRule annotationAdd
BLAST
Regioni214 – 468255Uroporphyrinogen-III C-methyltransferaseUniRule annotationAdd
BLAST
Regioni299 – 3013S-adenosyl-L-methionine bindingUniRule annotation
Regioni329 – 3302S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.UniRule annotation
In the C-terminal section; belongs to the precorrin methyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0007.
HOGENOMiHOG000290518.
KOiK02302.
OMAiLPVICEQ.
OrthoDBiEOG6DRPFR.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01646. Siroheme_synth.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C4LAG5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYLPLFARL NNRAVLLVGG GDIALRKARL LLDAGANLTV VAPSLHEELT
60 70 80 90 100
ELLVGHTYIP SRFTAAYLND QMLVIAATDD EEVNAEVAAA ADAANIWVNV
110 120 130 140 150
VDDPDRSSFI FPSIIDRSPI MVAVSSGGKA PVLVRMLRER LEALLPKHLG
160 170 180 190 200
ALANLSGEWR NQIKQKLGDI TSRRRFWEKA FASPQLATLL ETEQHDSAEQ
210 220 230 240 250
WMADQLQADD YAGGEIVLVG AGPGDAGLLT LKGLQQIQQA EVVLYDQLVS
260 270 280 290 300
PEVLNLVRRD AERISVGKKA GHHSVPQHEI NELLLSHARA GKRVVRLKGG
310 320 330 340 350
DPFMFGRGAE ELQAARAEGI PFSVVPGITA AAGATAYAGI PLTHRDSAQS
360 370 380 390 400
AVFITGHCKQ DGEEPDWAAL AASNQTLVIY MGLIGSPAIT ERLIAHGRRP
410 420 430 440 450
ETPVALIERG TTVRQRVLRG TLQELPELAK DAHSPSLIVI GEVAALADTL
460
SWFGGDTASG KEHLINLA
Length:468
Mass (Da):50,336
Last modified:July 7, 2009 - v1
Checksum:i51296415E4D733CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001616 Genomic DNA. Translation: ACQ94140.1.
RefSeqiYP_002893726.1. NC_012691.1.

Genome annotation databases

EnsemblBacteriaiACQ94140; ACQ94140; Tola_2546.
GeneIDi7883397.
KEGGitau:Tola_2546.
PATRICi23981759. VBITolAue42623_2528.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001616 Genomic DNA. Translation: ACQ94140.1.
RefSeqiYP_002893726.1. NC_012691.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi595494.Tola_2546.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACQ94140; ACQ94140; Tola_2546.
GeneIDi7883397.
KEGGitau:Tola_2546.
PATRICi23981759. VBITolAue42623_2528.

Phylogenomic databases

eggNOGiCOG0007.
HOGENOMiHOG000290518.
KOiK02302.
OMAiLPVICEQ.
OrthoDBiEOG6DRPFR.

Enzyme and pathway databases

UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.
BioCyciTAUE595494:GHEF-2627-MONOMER.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01646. Siroheme_synth.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 9187 / TA4.

Entry informationi

Entry nameiCYSG_TOLAT
AccessioniPrimary (citable) accession number: C4LAG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 7, 2009
Last modified: January 7, 2015
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.