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C4L8J2 (DDL_TOLAT) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Tola_0232
OrganismTolumonas auensis (strain DSM 9187 / TA4) [Complete proteome] [HAMAP]
Taxonomic identifier595494 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAeromonadalesAeromonadaceaeTolumonas

Protein attributes

Sequence length330 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 330330D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000202207

Regions

Domain120 – 326207ATP-grasp
Nucleotide binding150 – 20556ATP By similarity

Sites

Metal binding2801Magnesium or manganese 1 By similarity
Metal binding2931Magnesium or manganese 1 By similarity
Metal binding2931Magnesium or manganese 2 By similarity
Metal binding2951Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
C4L8J2 [UniParc].

Last modified July 7, 2009. Version 1.
Checksum: CD3113AF5071C6BC

FASTA33036,451
        10         20         30         40         50         60 
MKHLHVLLLC GGGGSEHEIS LLSANYLESQ LKELAEVSVT RVELFPDHWQ TNDGRSCHLG 

        70         80         90        100        110        120 
MDRQLHFANE AKPVDFVVPC IHGFPGETGD IQSMLELIGL PYLGCGSEGS KLCFNKVSTK 

       130        140        150        160        170        180 
LWLSALDIPN TPFLFLSDNN EAAHAQAHTA FRNWGAVFVK AASQGSSVGC YKVTDAAKLS 

       190        200        210        220        230        240 
EAVNAAFGYS DQVLVEKAVR PRELEVAVYR YNDQLVATRP GEIATPSDSF YSYEEKYSSG 

       250        260        270        280        290        300 
SNSTTYLEAP GLSDAQISTI REYALKAFTQ LGLKDLSRID FFLTEDNEIL LNEINTFPGM 

       310        320        330 
TPISMFPKLL EHHGDNFKQF LEGIIRSTVK 

« Hide

References

[1]"Complete sequence of Tolumonas auensis DSM 9187."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Spring S., Beller H.
Submitted (MAY-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 9187 / TA4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001616 Genomic DNA. Translation: ACQ91862.1.
RefSeqYP_002891448.1. NC_012691.1.

3D structure databases

ProteinModelPortalC4L8J2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING595494.Tola_0232.

Proteomic databases

PRIDEC4L8J2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACQ91862; ACQ91862; Tola_0232.
GeneID7885745.
KEGGtau:Tola_0232.
PATRIC23977057. VBITolAue42623_0236.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAMEFCVLF.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycTAUE595494:GHEF-249-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_TOLAT
AccessionPrimary (citable) accession number: C4L8J2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 7, 2009
Last modified: February 19, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways