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Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene

menD

Organism
Exiguobacterium sp. (strain ATCC BAA-1283 / AT1b)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).UniRule annotationSAAS annotation

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationSAAS annotation, Mn2+UniRule annotationSAAS annotation
  • thiamine diphosphateUniRule annotationSAAS annotationNote: Binds 1 thiamine pyrophosphate per subunit.UniRule annotationSAAS annotation
  • thiamine diphosphateNote: Binds 1 thiamine pyrophosphate per subunit.

Pathwayi

GO - Molecular functioni

  1. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. manganese ion binding Source: UniProtKB-HAMAP
  4. thiamine pyrophosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. menaquinone biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

TransferaseUniRule annotationSAAS annotation

Keywords - Biological processi

Menaquinone biosynthesisUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Thiamine pyrophosphateUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciESP360911:GI4R-2453-MONOMER.
UniPathwayiUPA00079.
UPA01057; UER00164.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotationSAAS annotation (EC:2.2.1.9UniRule annotationSAAS annotation)
Short name:
SEPHCHC synthaseUniRule annotation
Alternative name(s):
Menaquinone biosynthesis protein MenDUniRule annotation
Gene namesi
Name:menDUniRule annotation
Ordered Locus Names:EAT1b_2374Imported
OrganismiExiguobacterium sp. (strain ATCC BAA-1283 / AT1b)Imported
Taxonomic identifieri360911 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillales Family XII. Incertae SedisExiguobacterium
ProteomesiUP000000716: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi360911.EAT1b_2374.

Structurei

3D structure databases

ProteinModelPortaliC4L321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TPP enzyme family. MenD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1165.
HOGENOMiHOG000218359.
KOiK02551.
OMAiQKPWLLE.
OrthoDBiEOG6NWBQW.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
HAMAPiMF_01659. MenD.
InterProiIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view]
PfamiPF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004983. MenD. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00173. menD. 1 hit.

Sequencei

Sequence statusi: Complete.

C4L321-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKVLTEWVA SMVNRLVTSG VKDVVISPGS RSTPLAVAAY LHPQIRHHII
60 70 80 90 100
VDERSAGFFA LGLTRASETV RPVALICTSG TAATNYYSAV TEAFISQLPL
110 120 130 140 150
VVLTTDRPHE LRNVGAPQAI NQVRLYGEQV KTSVDLPVPE EATLSFMQQT
160 170 180 190 200
ITRFTNLSMQ APMGPVHLNV PFREPLLPDI ESLKQILKAD PVLPERQRIR
210 220 230 240 250
PTQADKDIFS ERTSHKQLAF VVGPYTPTSW QKSIVEYASE RQIPIFADPL
260 270 280 290 300
SGMRRFDGVL TNYDTWLASE HRTDWLPEVF VRFGAAPVSK RFNQWMAMAD
310 320 330 340 350
QLVIDEPGSY RDPSSRATIC YGDALDFLPL IASANEPRYL ERLRFFEEIA
360 370 380 390 400
ESAKSSLQGE VELTKRLLDT SIELLFVSNS MPIRDVDISL SAGTNIHVLA
410 420 430 440 450
NRGANGIDGV LSTALGSSYD TSHGALLIGD LAFYHDSNAL QLLKHHPGKF
460 470 480 490 500
SVVIANNNGG GIFSFLPQAA VDTQLFEDLF GTPLDLNIRG FADTYGLTYR
510 520 530 540
LIERPDEIQE AIEDGIHLIE FPSNREENVM IHRGWTQSVC SRLITENES
Length:549
Mass (Da):61,008
Last modified:July 7, 2009 - v1
Checksum:i78269CD75FC2CCEB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001615 Genomic DNA. Translation: ACQ71295.1.
RefSeqiWP_015880854.1. NC_012673.1.
YP_002886740.1. NC_012673.1.

Genome annotation databases

EnsemblBacteriaiACQ71295; ACQ71295; EAT1b_2374.
GeneIDi7870423.
KEGGieat:EAT1b_2374.
PATRICi21879986. VBIExiSp39724_2357.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001615 Genomic DNA. Translation: ACQ71295.1.
RefSeqiWP_015880854.1. NC_012673.1.
YP_002886740.1. NC_012673.1.

3D structure databases

ProteinModelPortaliC4L321.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi360911.EAT1b_2374.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACQ71295; ACQ71295; EAT1b_2374.
GeneIDi7870423.
KEGGieat:EAT1b_2374.
PATRICi21879986. VBIExiSp39724_2357.

Phylogenomic databases

eggNOGiCOG1165.
HOGENOMiHOG000218359.
KOiK02551.
OMAiQKPWLLE.
OrthoDBiEOG6NWBQW.

Enzyme and pathway databases

UniPathwayiUPA00079.
UPA01057; UER00164.
BioCyciESP360911:GI4R-2453-MONOMER.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
HAMAPiMF_01659. MenD.
InterProiIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view]
PfamiPF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004983. MenD. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00173. menD. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1283 / AT1bImported.

Entry informationi

Entry nameiC4L321_EXISA
AccessioniPrimary (citable) accession number: C4L321
Entry historyi
Integrated into UniProtKB/TrEMBL: July 7, 2009
Last sequence update: July 7, 2009
Last modified: March 4, 2015
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.