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Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene

menD

Organism
Exiguobacterium sp. (strain ATCC BAA-1283 / AT1b)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).UniRule annotationSAAS annotation

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotationSAAS annotation

Cofactori

Mn2+SAAS annotation

Pathwayi: 1,4-dihydroxy-2-naphthoate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate.UniRule annotationSAAS annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD)
  3. no protein annotated in this organism
  4. o-succinylbenzoate synthase (menC)
  5. 2-succinylbenzoate--CoA ligase (menE)
  6. 1,4-dihydroxy-2-naphthoyl-CoA synthase (menB)
  7. no protein annotated in this organism
This subpathway is part of the pathway 1,4-dihydroxy-2-naphthoate biosynthesis, which is itself part of Quinol/quinone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate, the pathway 1,4-dihydroxy-2-naphthoate biosynthesis and in Quinol/quinone metabolism.

Pathwayi: menaquinone biosynthesis

This protein is involved in the pathway menaquinone biosynthesis, which is part of Quinol/quinone metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationSAAS annotation
Biological processMenaquinone biosynthesisUniRule annotation
LigandMagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Thiamine pyrophosphateUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00079.
UPA01057; UER00164.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotationSAAS annotation (EC:2.2.1.9UniRule annotation)
Short name:
SEPHCHC synthaseUniRule annotation
Alternative name(s):
Menaquinone biosynthesis protein MenDUniRule annotation
Gene namesi
Name:menDUniRule annotation
Ordered Locus Names:EAT1b_2374Imported
OrganismiExiguobacterium sp. (strain ATCC BAA-1283 / AT1b)Imported
Taxonomic identifieri360911 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillales Family XII. Incertae SedisExiguobacterium
Proteomesi
  • UP000000716 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi360911.EAT1b_2374.

Structurei

3D structure databases

ProteinModelPortaliC4L321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 174TPP_enzyme_NInterPro annotationAdd BLAST166

Sequence similaritiesi

Belongs to the TPP enzyme family. MenD subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105C4A. Bacteria.
COG1165. LUCA.
HOGENOMiHOG000218359.
KOiK02551.
OMAiIFRILPG.
OrthoDBiPOG091H058U.

Family and domain databases

Gene3Di3.40.50.1220. 1 hit.
HAMAPiMF_01659. MenD. 1 hit.
InterProiView protein in InterPro
IPR029035. DHS-like_NAD/FAD-binding_dom.
IPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
PANTHERiPTHR42916. PTHR42916. 1 hit.
PfamiView protein in Pfam
PF02776. TPP_enzyme_N. 1 hit.
PIRSFiPIRSF004983. MenD. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00173. menD. 1 hit.

Sequencei

Sequence statusi: Complete.

C4L321-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKVLTEWVA SMVNRLVTSG VKDVVISPGS RSTPLAVAAY LHPQIRHHII
60 70 80 90 100
VDERSAGFFA LGLTRASETV RPVALICTSG TAATNYYSAV TEAFISQLPL
110 120 130 140 150
VVLTTDRPHE LRNVGAPQAI NQVRLYGEQV KTSVDLPVPE EATLSFMQQT
160 170 180 190 200
ITRFTNLSMQ APMGPVHLNV PFREPLLPDI ESLKQILKAD PVLPERQRIR
210 220 230 240 250
PTQADKDIFS ERTSHKQLAF VVGPYTPTSW QKSIVEYASE RQIPIFADPL
260 270 280 290 300
SGMRRFDGVL TNYDTWLASE HRTDWLPEVF VRFGAAPVSK RFNQWMAMAD
310 320 330 340 350
QLVIDEPGSY RDPSSRATIC YGDALDFLPL IASANEPRYL ERLRFFEEIA
360 370 380 390 400
ESAKSSLQGE VELTKRLLDT SIELLFVSNS MPIRDVDISL SAGTNIHVLA
410 420 430 440 450
NRGANGIDGV LSTALGSSYD TSHGALLIGD LAFYHDSNAL QLLKHHPGKF
460 470 480 490 500
SVVIANNNGG GIFSFLPQAA VDTQLFEDLF GTPLDLNIRG FADTYGLTYR
510 520 530 540
LIERPDEIQE AIEDGIHLIE FPSNREENVM IHRGWTQSVC SRLITENES
Length:549
Mass (Da):61,008
Last modified:July 7, 2009 - v1
Checksum:i78269CD75FC2CCEB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001615 Genomic DNA. Translation: ACQ71295.1.
RefSeqiWP_015880854.1. NC_012673.1.

Genome annotation databases

EnsemblBacteriaiACQ71295; ACQ71295; EAT1b_2374.
KEGGieat:EAT1b_2374.

Similar proteinsi

Entry informationi

Entry nameiC4L321_EXISA
AccessioniPrimary (citable) accession number: C4L321
Entry historyiIntegrated into UniProtKB/TrEMBL: July 7, 2009
Last sequence update: July 7, 2009
Last modified: September 27, 2017
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported