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C4L321

- C4L321_EXISA

UniProt

C4L321 - C4L321_EXISA

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Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene

menD

Organism
Exiguobacterium sp. (strain ATCC BAA-1283 / AT1b)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).UniRule annotationSAAS annotation

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Note: Binds 1 thiamine pyrophosphate per subunit.UniRule annotationSAAS annotation
  • Note: Magnesium or manganese.UniRule annotationSAAS annotation

Pathwayi

GO - Molecular functioni

  1. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. thiamine pyrophosphate binding Source: InterPro

GO - Biological processi

  1. menaquinone biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

TransferaseUniRule annotationSAAS annotation

Keywords - Biological processi

Menaquinone biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Thiamine pyrophosphateUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciESP360911:GI4R-2453-MONOMER.
UniPathwayiUPA00079.
UPA01057; UER00164.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotationSAAS annotation (EC:2.2.1.9UniRule annotationSAAS annotation)
Short name:
SEPHCHC synthaseUniRule annotation
Alternative name(s):
Menaquinone biosynthesis protein MenDUniRule annotation
Gene namesi
Name:menDUniRule annotation
Ordered Locus Names:EAT1b_2374Imported
OrganismiExiguobacterium sp. (strain ATCC BAA-1283 / AT1b)Imported
Taxonomic identifieri360911 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillales Family XII. Incertae SedisExiguobacterium
ProteomesiUP000000716: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi360911.EAT1b_2374.

Structurei

3D structure databases

ProteinModelPortaliC4L321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TPP enzyme family. MenD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1165.
HOGENOMiHOG000218359.
KOiK02551.
OMAiSMPVRDM.
OrthoDBiEOG6NWBQW.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
HAMAPiMF_01659. MenD.
InterProiIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view]
PfamiPF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004983. MenD. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00173. menD. 1 hit.

Sequencei

Sequence statusi: Complete.

C4L321-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKVLTEWVA SMVNRLVTSG VKDVVISPGS RSTPLAVAAY LHPQIRHHII
60 70 80 90 100
VDERSAGFFA LGLTRASETV RPVALICTSG TAATNYYSAV TEAFISQLPL
110 120 130 140 150
VVLTTDRPHE LRNVGAPQAI NQVRLYGEQV KTSVDLPVPE EATLSFMQQT
160 170 180 190 200
ITRFTNLSMQ APMGPVHLNV PFREPLLPDI ESLKQILKAD PVLPERQRIR
210 220 230 240 250
PTQADKDIFS ERTSHKQLAF VVGPYTPTSW QKSIVEYASE RQIPIFADPL
260 270 280 290 300
SGMRRFDGVL TNYDTWLASE HRTDWLPEVF VRFGAAPVSK RFNQWMAMAD
310 320 330 340 350
QLVIDEPGSY RDPSSRATIC YGDALDFLPL IASANEPRYL ERLRFFEEIA
360 370 380 390 400
ESAKSSLQGE VELTKRLLDT SIELLFVSNS MPIRDVDISL SAGTNIHVLA
410 420 430 440 450
NRGANGIDGV LSTALGSSYD TSHGALLIGD LAFYHDSNAL QLLKHHPGKF
460 470 480 490 500
SVVIANNNGG GIFSFLPQAA VDTQLFEDLF GTPLDLNIRG FADTYGLTYR
510 520 530 540
LIERPDEIQE AIEDGIHLIE FPSNREENVM IHRGWTQSVC SRLITENES
Length:549
Mass (Da):61,008
Last modified:July 7, 2009 - v1
Checksum:i78269CD75FC2CCEB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001615 Genomic DNA. Translation: ACQ71295.1.
RefSeqiWP_015880854.1. NC_012673.1.
YP_002886740.1. NC_012673.1.

Genome annotation databases

EnsemblBacteriaiACQ71295; ACQ71295; EAT1b_2374.
GeneIDi7870423.
KEGGieat:EAT1b_2374.
PATRICi21879986. VBIExiSp39724_2357.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001615 Genomic DNA. Translation: ACQ71295.1 .
RefSeqi WP_015880854.1. NC_012673.1.
YP_002886740.1. NC_012673.1.

3D structure databases

ProteinModelPortali C4L321.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 360911.EAT1b_2374.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACQ71295 ; ACQ71295 ; EAT1b_2374 .
GeneIDi 7870423.
KEGGi eat:EAT1b_2374.
PATRICi 21879986. VBIExiSp39724_2357.

Phylogenomic databases

eggNOGi COG1165.
HOGENOMi HOG000218359.
KOi K02551.
OMAi SMPVRDM.
OrthoDBi EOG6NWBQW.

Enzyme and pathway databases

UniPathwayi UPA00079 .
UPA01057 ; UER00164 .
BioCyci ESP360911:GI4R-2453-MONOMER.

Family and domain databases

Gene3Di 3.40.50.970. 2 hits.
HAMAPi MF_01659. MenD.
InterProi IPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view ]
Pfami PF02776. TPP_enzyme_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF004983. MenD. 1 hit.
SUPFAMi SSF52518. SSF52518. 2 hits.
TIGRFAMsi TIGR00173. menD. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1283 / AT1bImported.

Entry informationi

Entry nameiC4L321_EXISA
AccessioniPrimary (citable) accession number: C4L321
Entry historyi
Integrated into UniProtKB/TrEMBL: July 7, 2009
Last sequence update: July 7, 2009
Last modified: November 26, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3