C4KZS1 (C4KZS1_EXISA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108
EC=2.7.7.60 HAMAP MF_00108

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase HAMAP MF_00108
MEP cytidylyltransferase HAMAP MF_00108

Gene names

Name:	ispD HAMAP MF_00108
Ordered Locus Names:	EAT1b_1658

Organism

Exiguobacterium sp. (strain ATCC BAA-1283 / AT1b) [Complete proteome] [HAMAP] EMBL ACQ70584.1

Taxonomic identifier

360911 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillales Family XII. Incertae Sedis › Exiguobacterium

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108 SAAS SAAS018294
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108 SAAS SAAS018294
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108 SAAS SAAS018294
Sequence similarities	Belongs to the IspD family. HAMAP MF_00108

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00108 SAAS SAAS018294
Molecular function	Nucleotidyltransferase SAAS SAAS018294 HAMAP MF_00108 EMBL ACQ70584.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Site	16	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	23	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	150	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108
	Site	206	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108

Sequences

	Sequence		Length	Mass (Da)
	C4KZS1 [UniParc]. Last modified July 7, 2009. Version 1. Checksum: 32D548129C710BD4	FASTA	226	25,229
	10 20 30 40 50 60 MVAYTVVIPA AGRGKRMGAD ANKLMLTLRD KPIIAWTLQA FDTDPWCERM VLAIRPEERD 70 80 90 100 110 120 WFEAMTSQMK TPVTYVAGGE ERQQSVHAGL KVVAPDTIVM IHDGARPFVK REQLHQVAEA 130 140 150 160 170 180 ALQGGAILAV PVKDTVKQVQ RHHIERTVPR ENLWLAQTPQ AFQAKAILAA HERAVEQQVM 190 200 210 220 GTDDASLFEW LGESVQVVLG DYHNIKMTTP EDLLFGDAIL AKEELQ

References

[1]

"Complete sequence of Exiguobacterium sp. AT1b." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G. , Ramaley R.F., Rodrigues D.F., Vishnivetskaya T.A., Kathariou S., Tiedje J.M., Richardson P. Submitted (MAY-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1283 / AT1b.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001615 Genomic DNA. Translation: ACQ70584.1.
RefSeq	YP_002886029.1. NC_012673.1.
3D structure databases
ProteinModelPortal	C4KZS1.
ModBase	Search...
Protein-protein interaction databases
STRING	C4KZS1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7869719.
GenomeReviews	Gene locus EAT1b_1658 in contig CP001615_GR.
KEGG	eat:EAT1b_1658.
PATRIC	21878430. VBIExiSp39724_1638.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	SKVIVVC.
ProtClustDB	CLSK2488855.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C4KZS1_EXISA

Accession

Primary (citable) accession number: C4KZS1

Entry history

Integrated into UniProtKB/TrEMBL:	July 7, 2009
Last sequence update:	July 7, 2009
Last modified:	December 14, 2011
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)