C4KZE2 (THIM_EXISA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyethylthiazole kinase
EC=2.7.1.50

Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase

Gene names

Name:	thiM
Ordered Locus Names:	EAT1b_1529

Organism

Exiguobacterium sp. (strain ATCC BAA-1283 / AT1b) [Complete proteome] [HAMAP]

Taxonomic identifier

360911 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillales incertae sedis › Bacillales Family XII. Incertae Sedis › Exiguobacterium

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228
Cofactor	Magnesium By similarity. HAMAP-Rule MF_00228
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW thiamine diphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Hydroxyethylthiazole kinase HAMAP-Rule MF_00228		PRO_0000383861
Sites
	Binding site	41	1	Substrate; via amide nitrogen By similarity
	Binding site	117	1	ATP By similarity
	Binding site	163	1	ATP By similarity
	Binding site	190	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	C4KZE2 [UniParc]. Last modified July 7, 2009. Version 1. Checksum: D4C576B23C7B8993	FASTA	263	27,753
	10 20 30 40 50 60 MRHEIWNEIR QQAPLIHNIT NTVVANFSAN GLLAIGASPV MADAIEEVAE MTFVSDALVL 70 80 90 100 110 120 NIGTLGTMTE ATMIRAGQAA NEAGCPIVLD PVGVGATSFR RDVVERIMRH VRVSVIRGNA 130 140 150 160 170 180 GEIATLIGVD WAARGVDAGD GACEPRQLAL EAAKRYGCVV AVTGKEDMVS DGHVVMEVRG 190 200 210 220 230 240 GTKRMTETTG TGCLLSAVVG ACLAVEKNAL KATVTAMSGY AYCGELASER AEGPGDFPIH 250 260 FLNALHQLTQ YTIPTNRIEV STS

References

[1]

"Complete sequence of Exiguobacterium sp. AT1b." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G. , Ramaley R.F., Rodrigues D.F., Vishnivetskaya T.A., Kathariou S., Tiedje J.M., Richardson P. Submitted (MAY-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1283 / AT1b.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001615 Genomic DNA. Translation: ACQ70455.1.
RefSeq	YP_002885900.1. NC_012673.1.
3D structure databases
ProteinModelPortal	C4KZE2.
ModBase	Search...
Protein-protein interaction databases
STRING	360911.EAT1b_1529.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACQ70455; ACQ70455; EAT1b_1529.
GeneID	7869845.
KEGG	eat:EAT1b_1529.
PATRIC	21878144. VBIExiSp39724_1508.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2145.
HOGENOM	HOG000114352.
KO	K00878.
OMA	SPVMAHA.
Enzyme and pathway databases
BioCyc	ESP360911:GI4R-1535-MONOMER.
UniPathway	UPA00060; UER00139.
Family and domain databases
HAMAP	MF_00228. Thz_kinase.
InterPro	IPR000417. Hyethyz_kinase. IPR026012. Hyethyz_kinase/Th_synth. [Graphical view]
PANTHER	PTHR20857:SF14. PTHR20857:SF14. 1 hit.
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
TIGRFAMs	TIGR00694. thiM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIM_EXISA

Accession

Primary (citable) accession number: C4KZE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 7, 2009
Last modified:	May 1, 2013
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program