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C4KP90 (C4KP90_BURPE) Unreviewed, UniProtKB/TrEMBL

Last modified May 14, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme HAMAP-Rule MF_00277

Short name=UTase/UR HAMAP-Rule MF_00277
Alternative name(s):
Bifunctional [protein-PII] modification enzyme HAMAP-Rule MF_00277
Bifunctional nitrogen sensor protein HAMAP-Rule MF_00277
Gene names
Name:glnD HAMAP-Rule MF_00277 EMBL ACQ95234.1
ORF Names:GBP346_A2565 EMBL ACQ95234.1
OrganismBurkholderia pseudomallei MSHR346 [Complete proteome] EMBL ACQ95234.1
Taxonomic identifier536230 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group

Protein attributes

Sequence length858 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen metabolism By similarity. HAMAP-Rule MF_00277

Catalytic activity

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII]. HAMAP-Rule MF_00277 SAAS SAAS002912

Uridylyl-[protein-PII] + H2O = UMP + [protein-PII]. HAMAP-Rule MF_00277 SAAS SAAS002912

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00277 SAAS SAAS002912

Enzyme regulation

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity. HAMAP-Rule MF_00277

Domain

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity. HAMAP-Rule MF_00277

Sequence similarities

Belongs to the GlnD family. HAMAP-Rule MF_00277

Contains 1 HD domain. HAMAP-Rule MF_00277

Contains 2 ACT domains. HAMAP-Rule MF_00277

Contains ACT domains. SAAS SAAS002912

Contains HD domain. SAAS SAAS002912

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain444 – 550107HD By similarity HAMAP-Rule MF_00277
Domain682 – 76180ACT 1 By similarity HAMAP-Rule MF_00277
Region1 – 324324Uridylyltransferase By similarity HAMAP-Rule MF_00277
Region325 – 681357Uridylyl-removing By similarity HAMAP-Rule MF_00277

Sequences

Sequence LengthMass (Da)Tools
C4KP90 [UniParc].

Last modified July 7, 2009. Version 1.
Checksum: E71C618DBA6FE7F4

FASTA85896,750
        10         20         30         40         50         60 
MSASVAEPPP ALSRKAEFKA AKAELLARFK SANHVTPLMH ALSRATDDAL RSLWQECGLP 

        70         80         90        100        110        120 
ATLALVAVGG FGRGELSPHS DVDILVLLPD AHASELDERI ERFIGMAWDL GLEIGSSVRT 

       130        140        150        160        170        180 
VDQCIEEASH DVTVQTSLLE ARRIVGSTAL FERFMLRYRE ALDARAFFQA KVLEMRQRHA 

       190        200        210        220        230        240 
KFQDTPYSLE PNVKESPGGL RDLQTILWIA RAAGFGSSWR ELDTRGLITD REARELRRNE 

       250        260        270        280        290        300 
GFLKTLRARL HVIAGRRQDI LVFDLQTQAA ESFGYQPTSA KRASEQLMRR YYWAAKAVTQ 

       310        320        330        340        350        360 
LATILIQNIE AQLFPATSGV TRVLSPGRFV EKQGMLEIAA DDVFERHPDA ILEAFLLYEA 

       370        380        390        400        410        420 
TRGVKGLSAR TLRALYNSRD VMNNAWRRDP RNRHTFMQIL QQPEGITHAF RLMNQTSVLG 

       430        440        450        460        470        480 
RYLLNFRRIV GQMQHDLYHV YTVDQHILMV LRNIRRFAVA EHAHEYPFCS QLIVNFERPW 

       490        500        510        520        530        540 
VLYVAALFHD IAKGRGGDHS ALGMADARRF CREHGIEGDD AALVVWLVQH HLTMSQVAQK 

       550        560        570        580        590        600 
QDTSDPVVIK RFAELVGSER RLTALYLLTV ADIRGTSPKV WNTWKGKLLE DLYRATLAVL 

       610        620        630        640        650        660 
GGAQPDAHSE LKTRQEEALA LLRLETVPPD AHRALWDQLD VGYFLRHDAA DIAWQTRVLY 

       670        680        690        700        710        720 
RHVAADTAIV RARPSPVGDA LQVLVYVKDR SDLFAGICAY FDRNGLSVLD ARVNTTRHGY 

       730        740        750        760        770        780 
ALDNFIVTQT EHDVQYRDIA NLVEQQLAAR LAESAPLPEP SKGRLSRLSR TFPITPRVDL 

       790        800        810        820        830        840 
RADERGQYYI LSVSANDRPG LLYSIARVLA EHRVGVHAAR INTLGERVED VFMLDGTGLS 

       850 
DNRLQIQVET ELLRAIAV 

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References

[1]Harkins D.M., Brinkac L.M., Rogers Y.C., Detter J.C., Munk A.C., Bruce D.C., Keim P., Sims D.R., Brettin T.S.
Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: MSHR346 EMBL ACQ95234.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001408 Genomic DNA. Translation: ACQ95234.1.
RefSeqYP_002897262.1. NC_012695.1.

3D structure databases

ProteinModelPortalC4KP90.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING536230.GBP346_A2565.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACQ95234; ACQ95234; GBP346_A2565.
GeneID7887285.
KEGGbpr:GBP346_A2565.
PATRIC32574039. VBIBurPse130472_2341.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2844.
HOGENOMHOG000261778.
KOK00990.
OrthoDBEOG6CCH44.

Enzyme and pathway databases

BioCycBPSE536230:GHVQ-2557-MONOMER.

Family and domain databases

Gene3D1.10.3210.10. 1 hit.
HAMAPMF_00277. PII_uridylyl_transf.
InterProIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFPIRSF006288. PII_uridyltransf. 1 hit.
SMARTSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsTIGR01693. UTase_glnD. 1 hit.
PROSITEPS51671. ACT. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC4KP90_BURPE
AccessionPrimary (citable) accession number: C4KP90
Entry history
Integrated into UniProtKB/TrEMBL: July 7, 2009
Last sequence update: July 7, 2009
Last modified: May 14, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)