C4K8Y6 (DUT_HAMD5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	HDEF_2318

Organism

Hamiltonella defensa subsp. Acyrthosiphon pisum (strain 5AT) [Complete proteome] [HAMAP]

Taxonomic identifier

572265 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › unclassified Enterobacteriaceae › ant, tsetse, mealybug, aphid, etc. endosymbionts › aphid secondary symbionts › Candidatus Hamiltonella › Candidatus Hamiltonella defensa

Protein attributes

Sequence length	151 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP-Rule MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP-Rule MF_00116
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP-Rule MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dUMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 151	151	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP-Rule MF_00116		PRO_1000202985
Regions
	Region	70 – 72	3	Substrate binding By similarity
	Region	87 – 89	3	Substrate binding By similarity
Sites
	Binding site	83	1	Substrate By similarity
	Binding site	97	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	C4K8Y6 [UniParc]. Last modified July 7, 2009. Version 1. Checksum: D8654FAD1125E900	FASTA	151	16,297
	10 20 30 40 50 60 MKKIDIKILD KRIGDVFPLP TYTTSGSAGL DLRVCTQAPQ HLSPGETRLL PTGLAVHIAD 70 80 90 100 110 120 PHLAAMILPR SGLGHKNGIV LGNLVGLIDS DYQGELMLSV WNRSQIDFLI NPGDRLAQMV 130 140 150 FVPVVQVELN IVSEFTSSQR GSGGFGHSGR Q

References

[1]

"Hamiltonella defensa, genome evolution of protective bacterial endosymbiont from pathogenic ancestors." Degnan P.H., Yu Y., Sisneros N., Wing R.A., Moran N.A. Proc. Natl. Acad. Sci. U.S.A. 106:9063-9068(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 5AT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001277 Genomic DNA. Translation: ACQ68855.1.
RefSeq	YP_002925003.1. NC_012751.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	572265.HDEF_2318.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACQ68855; ACQ68855; HDEF_2318.
GeneID	7951913.
KEGG	hde:HDEF_2318.
PATRIC	31976093. VBICanHam112931_2292.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0756.
KO	K01520.
OMA	ILPRSGM.
Enzyme and pathway databases
BioCyc	HDEF572265:GJAB-2340-MONOMER.
UniPathway	UPA00610; UER00666.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact.
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_HAMD5

Accession

Primary (citable) accession number: C4K8Y6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 7, 2009
Last modified:	May 1, 2013
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program