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C4K7R7 (SYL_HAMD5) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:HDEF_2038
OrganismHamiltonella defensa subsp. Acyrthosiphon pisum (strain 5AT) [Complete proteome] [HAMAP]
Taxonomic identifier572265 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeaphid secondary symbiontsCandidatus Hamiltonella

Protein attributes

Sequence length869 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 869869Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000202222

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif620 – 6245"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6231ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
C4K7R7 [UniParc].

Last modified July 7, 2009. Version 1.
Checksum: 0A84DC84D4B2F361

FASTA869100,422
        10         20         30         40         50         60 
MQDIYRHQDI ESHVQRHWEQ TQTFTVKEDP KKEKYYCLAM WPYPSGRLHM GHVRNYTITD 

        70         80         90        100        110        120 
VIARYQRMLG KNVLQPMGWD AFGLPAEGAA IKNKTSPIDW TKANIQYMKK QLQSLGFAYD 

       130        140        150        160        170        180 
WSRELTSCKP EYYRWEQWFF TQLYEKGLVY KKTSDVNWCP QDFTVLANEQ VIDGRCWRCD 

       190        200        210        220        230        240 
SKVERKSIPQ WFIKITAYAD QLLDDLDELE HWPEQVKTMQ RHWIGRSEGV EIRFPLDHDE 

       250        260        270        280        290        300 
KTDLTVYSTR PDTLMGVAFI AIAPDHFFSA EIAKNVPALA TFIEECRHIK MAEAETATIE 

       310        320        330        340        350        360 
KKGIDTGFFA LHPLTGKKIP IWVANFVLME YGTGAVMGVP GHDQRDWEFA TQYHLPIKAV 

       370        380        390        400        410        420 
ILLEDGTEPD VQKKPLIEKG RLCHSGEFNG LSYQESCDRI IDKLVDLGTG QRKVNYRLRD 

       430        440        450        460        470        480 
WGVSRQRYWG APIPMITLED GRIIGTPEDQ LPVILPEKTL IKDMINPLKA DQDWAKTSVA 

       490        500        510        520        530        540 
GQLGIRETDT FDTFIESSWY YARYACPKYD QGMIEKAAAN YWLPVDQYVG GIEHAIMHLL 

       550        560        570        580        590        600 
YFRFFHKLMR DQGLVDSKEP AKRLLCQGMV LADAFYYNAQ NGERVWVSPT EVTVERDNKG 

       610        620        630        640        650        660 
QFLNAFDAQG RDLIHAGMSK MSKSKNNGID PQAIVEKYGA DTVRLFMMFA SAPEMTLEWQ 

       670        680        690        700        710        720 
ESGLEGAYRF LKRLWRFVFD HVIQGPTQPL KKENLNSTQK NLRRNLHKTI AKVTDDIGRR 

       730        740        750        760        770        780 
QTFNTAIAAI MELMNQLYRA PTNTEQDRAL IQEACISVIK MLYPFTPHIS FILWQHLHQS 

       790        800        810        820        830        840 
PDETHPLNID DSLWPVVDQN ALIEDETLVV IQINGKMRAK ITVPMNSTQQ EVYESALQEP 

       850        860 
TVIKHLKGIT PCHVIYVPNK LLNLVVNNE 

« Hide

References

[1]"Hamiltonella defensa, genome evolution of protective bacterial endosymbiont from pathogenic ancestors."
Degnan P.H., Yu Y., Sisneros N., Wing R.A., Moran N.A.
Proc. Natl. Acad. Sci. U.S.A. 106:9063-9068(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 5AT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001277 Genomic DNA. Translation: ACQ68610.1.
RefSeqYP_002924758.1. NC_012751.1.

3D structure databases

ProteinModelPortalC4K7R7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING572265.HDEF_2038.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACQ68610; ACQ68610; HDEF_2038.
GeneID7951634.
KEGGhde:HDEF_2038.
PATRIC31975537. VBICanHam112931_2018.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycHDEF572265:GJAB-2061-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_HAMD5
AccessionPrimary (citable) accession number: C4K7R7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 7, 2009
Last modified: April 16, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries