Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

C4K734 (DDL_HAMD5) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:HDEF_1781
OrganismHamiltonella defensa subsp. Acyrthosiphon pisum (strain 5AT) [Complete proteome] [HAMAP]
Taxonomic identifier572265 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeaphid secondary symbiontsCandidatus Hamiltonella

Protein attributes

Sequence length320 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 320320D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000202200

Regions

Domain101 – 317217ATP-grasp
Nucleotide binding148 – 20356ATP By similarity

Sites

Metal binding2711Magnesium or manganese 1 By similarity
Metal binding2841Magnesium or manganese 1 By similarity
Metal binding2841Magnesium or manganese 2 By similarity
Metal binding2861Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
C4K734 [UniParc].

Last modified July 7, 2009. Version 1.
Checksum: DB889E36FA65CBF2

FASTA32035,514
        10         20         30         40         50         60 
MPEKVAVLLG GTSSERQISL QSGHAVVAGL REAGIDARPI DTKNFLVTEL KEKGFTKAFI 

        70         80         90        100        110        120 
ALHGRDGEDG HLQAVLEFLK IPYTGSGVMA SALAMDKHRS KMIFQGAGLP VSPYVALNRE 

       130        140        150        160        170        180 
QIWPNVTQNT SNDLLLNNRI SNKLIKNINQ LGLPLIVKPS REGSSFGMTK VEHLDQLDDA 

       190        200        210        220        230        240 
LKKAWHYDEE ILVEKWHFGT ELTVAILGDT VLPSIRIQVS DIFYDYQAKY VSDKTQYFCP 

       250        260        270        280        290        300 
SGLKQEQEKQ LATLSMNAYR ALGCDGWGRV DVMLDDEGHF YLMEINTAPG MTDHSLFPMA 

       310        320 
ARQAGFSFSE LVCKILSLAH 

« Hide

References

[1]"Hamiltonella defensa, genome evolution of protective bacterial endosymbiont from pathogenic ancestors."
Degnan P.H., Yu Y., Sisneros N., Wing R.A., Moran N.A.
Proc. Natl. Acad. Sci. U.S.A. 106:9063-9068(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 5AT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001277 Genomic DNA. Translation: ACQ68377.1.
RefSeqYP_002924525.1. NC_012751.1.

3D structure databases

ProteinModelPortalC4K734.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING572265.HDEF_1781.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACQ68377; ACQ68377; HDEF_1781.
GeneID7951378.
KEGGhde:HDEF_1781.
PATRIC31975020. VBICanHam112931_1765.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
KOK01921.
OMAANDTQYR.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycHDEF572265:GJAB-1797-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_HAMD5
AccessionPrimary (citable) accession number: C4K734
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 7, 2009
Last modified: May 14, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways