C4K6Y1 (FMT_HAMD5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase
EC=2.1.2.9

Gene names

Name:	fmt
Ordered Locus Names:	HDEF_1721

Organism

Hamiltonella defensa subsp. Acyrthosiphon pisum (strain 5AT) [Complete proteome] [HAMAP]

Taxonomic identifier

572265 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › unclassified Enterobacteriaceae › ant, tsetse, mealybug, aphid, etc. endosymbionts › aphid secondary symbionts › Candidatus Hamiltonella › Candidatus Hamiltonella defensa

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182
Sequence similarities	Belongs to the fmt family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from electronic annotation. Source: GOC
Molecular_function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182		PRO_1000203862
Regions
	Region	113 – 116	4	Tetrahydrofolate (THF) binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	C4K6Y1 [UniParc]. Last modified July 7, 2009. Version 1. Checksum: 2E12DE7552ABA9CF	FASTA	319	35,594
	10 20 30 40 50 60 MSESLKIIFA GTPDFSACHL QHLLSHRQKI LGVFTQPDRP AGRGKKLAFS PVKILATQHH 70 80 90 100 110 120 IPVYQPHSLG LKEEQQSILD LDADVMVVVA YGLLLPQAVL NMPRLGCINV HPSLLPRWRG 130 140 150 160 170 180 AAPIQRAIWA GDQETGVTIM QMDSGLDTGN MLYKTVYPIQ PDDTGASLQA KLAALGSQDL 190 200 210 220 230 240 LLTLKKMAEG KMHGETQDEQ KTTYAHKLTK KEARLDWLLP AAHLERCVRA FNPWPVSYFI 250 260 270 280 290 300 INEQIIKVWE AQAMPDSQQV SHLQPGTVLK ADKNGIQILT SEGVLNMTKF QLPGKKIISA 310 WDFLNSRNEW FQIGKQLLS

References

[1]

"Hamiltonella defensa, genome evolution of protective bacterial endosymbiont from pathogenic ancestors." Degnan P.H., Yu Y., Sisneros N., Wing R.A., Moran N.A. Proc. Natl. Acad. Sci. U.S.A. 106:9063-9068(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 5AT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001277 Genomic DNA. Translation: ACQ68324.1.
RefSeq	YP_002924472.1. NC_012751.1.
3D structure databases
ProteinModelPortal	C4K6Y1.
ModBase	Search...
Protein-protein interaction databases
STRING	572265.HDEF_1721.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACQ68324; ACQ68324; HDEF_1721.
GeneID	7951318.
KEGG	hde:HDEF_1721.
PATRIC	31974901. VBICanHam112931_1711.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0223.
KO	K00604.
OMA	GITLMQM.
Enzyme and pathway databases
BioCyc	HDEF572265:GJAB-1737-MONOMER.
Family and domain databases
Gene3D	3.10.25.10. 1 hit. 3.40.50.170. 1 hit.
HAMAP	MF_00182. Formyl_trans.
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
PANTHER	PTHR11138. PTHR11138. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. fmt. 1 hit.
PROSITE	PS00373. GART. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FMT_HAMD5

Accession

Primary (citable) accession number: C4K6Y1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	July 7, 2009
Last modified:	May 1, 2013
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program